Method for preparing protodioscin

A technology of protodioscin and eluent, applied in the field of medicine, can solve problems such as difficulty in meeting scientific research or new drug research and development needs, low yield of protodioscin, limitation of in-depth research, etc., and achieves easy control of operating process conditions, removal of impurities and pigments. The effect of good effect and simple preparation method

Inactive Publication Date: 2015-08-26
JIANGSU YONGJIAN PHARMA TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The yield of protodioscin on the market is very low, and the yield rate is small, which is difficult to m

Method used

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  • Method for preparing protodioscin
  • Method for preparing protodioscin

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specific Embodiment 1

[0036] Prepare 150kg of dried pangolina medicinal material, crush it into a coarse powder, put it into a stainless steel traditional Chinese medicine extraction tank, use a 70% ethanol solution by mass percentage, heat and reflux for extraction 3 times, 8 times the amount each time, and 2 hours each time; secondly, combine Extract, filter, and concentrate the filtrate under reduced pressure to a relative density of 1.08 (40°C), let it cool, let stand overnight, then filter, and the filtrate is subjected to AB-8 macroporous adsorption resin column chromatography, the macroporous adsorption resin column The column volume of the adsorption resin is 200L (column diameter 0.4m, column height 1.6m), where the column volume refers to the volume from the bottom plate of the column to the resin deposition surface after the macroporous adsorption resin is loaded into the column; the volume of the resin in the resin column is the same as The ratio of medicinal material weight (dry weight)...

specific Embodiment 2

[0037] Prepare 90kg of dried pangolina medicinal material, crush it into coarse powder, put it into a stainless steel traditional Chinese medicine extraction tank, use a 50% ethanol solution by mass percentage, heat and reflux extract 3 times, 8 times the amount each time, 2 hours each time. Combine the extracts, filter, and concentrate the filtrate under reduced pressure to a relative density of 1.01 (40°C), let it cool, let it stand overnight, filter, and the filtrate is subjected to AB-8 macroporous adsorption resin column chromatography. The column volume of the macroporous adsorption resin is 120L, where the column volume refers to the volume from the bottom plate of the column to the gel deposition surface after the macroporous adsorption resin is loaded into the column. After the filtrate flows into the resin column completely, first wash with 2.5 times of column volume of water until the effluent is clear and transparent, then use 3 times of the column volume to elute a...

specific Embodiment 3

[0038] Prepare 120kg of dried pangolins, crush them into coarse powder, put them into a stainless steel traditional Chinese medicine extraction tank, and use 70% ethanol solution by mass percentage to extract by heating and refluxing 3 times, 8 times the amount each time, 2 hours each time. Combine the extracts, filter, and concentrate the filtrate under reduced pressure to a relative density of 1.05 (40°C), let it cool, let it stand overnight, filter, and the filtrate is subjected to AB-8 macroporous adsorption resin column chromatography. The column volume of the macroporous adsorption resin is 160L, where the column volume refers to the volume from the bottom plate of the column to the gel deposition surface after the macroporous adsorption resin is loaded into the column; Wash with water until the effluent is clear and transparent, then use 3 times the column volume of ethanol solution with a mass percent concentration of 20% to elute and wash until the effluent is clear an...

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Abstract

The invention belongs to the technical field of medicine, relates to a method for separation extraction of a compound in the chemical field and especially relates to a preparation method of a plant natural pharmacological active substance protodioscin. The method utilizes a rhizoma dioscoreae nipponicae medical material as a raw material and utilizes a column chromatography method to purify protodioscin. The method has the advantages of simple refining process, operation easiness, high purification efficiency and short refining time.

Description

technical field [0001] The invention belongs to the technical field of medicine, relates to a method for separating and extracting compounds in the chemical field, and in particular to a method for preparing protodioscin, a plant natural pharmacologically active substance. Background technique [0002] Chuanshanlong is the dried rhizome of Dioscorea anipponica Makino, a plant of the Dioscoreaceae family. It is warm in nature, sweet and bitter in taste, and returns to the liver, kidney, and lung meridian. Folks often use it to soak in wine or decoct it for treatment of rheumatism, joint swelling and pain, bruises and shortness of breath. Modern studies have shown that pangolin has various pharmacological effects such as regulating immunity, improving cardiovascular function, antitussive, asthmatic, and expectorant. It is clinically used to treat rheumatoid arthritis, coronary heart disease, hypertension, myocardial infarction, and bronchial asthma. The main active ingredient...

Claims

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Application Information

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IPC IPC(8): C07J71/00
CPCC07J71/001
Inventor 顾正兵张杰
Owner JIANGSU YONGJIAN PHARMA TECH CO LTD
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