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Application of total saponin of Dioscorea in preparation of drugs for controlling diabetic nephropathy

A technology of total saponins and saponins, applied in the field of application of total saponins of saponins in the preparation of drugs for the prevention and treatment of diabetic nephropathy, can solve the problems of undiscovered diabetic nephropathy, reduce the degree of renal fibrosis, and reduce the increase of serum creatinine and blood urea nitrogen , reduce the effect of kidney hypertrophy

Inactive Publication Date: 2016-01-27
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report on the treatment of diabetic nephropathy with total saponins of Hessian

Method used

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  • Application of total saponin of Dioscorea in preparation of drugs for controlling diabetic nephropathy
  • Application of total saponin of Dioscorea in preparation of drugs for controlling diabetic nephropathy
  • Application of total saponin of Dioscorea in preparation of drugs for controlling diabetic nephropathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The preparation method of the total saponins of Hexa sativa:

[0017] Take 1kg of Chinese herbal medicine, add 10L of 70% ethanol to reflux for extraction for 2 hours, filter to obtain the filtrate; the filter residue is then reflux extracted with 70% ethanol twice, each time for 2 hours, filter, combine the filtrate, and recover the filtrate under reduced pressure until it has no alcohol smell , add water to be mixed with the medicinal liquid that contains 0.1g medicinal material / ml, go up AB-8 macroporous resin column with the flow velocity of 2BV / h, with the ethanol of 50% and 70% ethanol successively with the flow velocity elution of 2BV / h, discard Remove 50% ethanol eluate, collect 70% ethanol eluate, reclaim the solvent to nearly dryness, and dry under reduced pressure to obtain 63 g of total saponins of scorpion.

Embodiment 2

[0019] Experimental Study on the Treatment of Diabetic Nephropathy with Total Saponins of Lemongrass

[0020] 1. Experimental materials:

[0021] 1.1 Reagents: Metformin Hydrochloride Tablets (Jingfeng Pharmaceutical Co., Ltd.); Xiaoke Pills (Guangzhou Baiyunshan Zhongyi Pharmaceutical Co., Ltd.); STZ (Sigma-Aldrich Company; batch number: 876232); urine protein assay kit (Nanjing Jiancheng Biology Co., Ltd.) Engineering Research Institute); Burea Nitrogen Determination Kit (Nanjing Jiancheng Bioengineering Research Institute); Creatinine Determination Kit (Nanjing Jiancheng Bioengineering Research Institute); Glucose Determination Kit (Nanjing Jiancheng Bioengineering Research Institute); Rat Tumor Necrosis Factor -α (TNF-α) assay kit (MerckMilliporeInc); rat interleukin 1β (IL-1β) assay kit (MerckMilliporeInc); rat interleukin 6 (IL-6) assay kit (MerckMilliporeInc).

[0022] 1.2 Animals: Adult male SD rats (180-220g, Animal Quality Certificate No. SCKX2008-0010), purchased f...

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PUM

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Abstract

The invention discloses a preparation method and application of an effective part of total saponin of Dioscorea. The effective part is composed of protodioscin, methyl protodioscin, gracillin, protogracillin, dioscin and diosgenin, and can be used alone or in a mixture with a pharmaceutically acceptable carrier to prepare the drugs for controlling diabetic nephropathy. Efficacy experiments show that the effective part of total saponin of Dioscorea can give the evident reduction in blood glucose, kidney hypertrophy level and urine protein discharge and delaying of urine protein formation, and is functional in repairing kidney injury caused by diabetics.

Description

Technical field: [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to the application of total saponins of scorpions in the preparation of drugs for preventing and treating diabetic nephropathy. Background technique: [0002] Diabetic nephropathy (diabetic nephropathy) refers to glomerulosclerosis caused by diabetic microvascular disease, also known as diabetic glomerulosclerosis, diabetic nephropathy is one of the microvascular complications of diabetes. Diabetic nephropathy is the most common complication of diabetes, and its incidence is increasing year by year with the rapid growth of diabetes. In 2001, the prevalence of type 2 diabetes complicated with nephropathy in my country was 34.7%, and the incidence of diabetic nephropathy has been increasing in the past 20 years. Diabetic nephropathy has become one of the main causes of end-stage renal disease. At present, clinical drugs for the treatment of diabetic ...

Claims

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Application Information

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IPC IPC(8): A61K36/8945A61P3/10A61P13/12
Inventor 郭常润
Owner CHINA PHARM UNIV
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