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Method for extracting protodioscin from fenugreek seeds

A technology of protodioscin and fenugreek, which is applied in the field of extracting protodioscin, can solve the problems of low protodioscin content, affecting the efficacy of use, and high production costs, and achieves the effects of low cost, improved purity, and low requirements for production equipment

Inactive Publication Date: 2015-07-22
ZHEJIANG HUISONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The content of protodioscin in the fenugreek water extract prepared above is low, the yield is low, and there are many impurities, which affect the efficacy of use, and the production cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 100 kg of fenugreek seeds were pressed with a screw oil press at a pressing temperature of 10° C. and a pressing pressure of 40 MPa to remove lipid components to obtain fenugreek seed cakes. The fenugreek seed cake was leached with 6 times of purified water, the leaching temperature was 50°C, and the leaching time was 1 hour, the extract was filtered to obtain the filtrate, and the purified water was leached again in the above-mentioned manner, and the filtrates were combined. Purify the combined filtrate with a CAD-40 macroporous adsorption resin column at a flow rate of 1BV / h, wherein the amount of resin is 3 times the amount of crude drug, and the diameter-height ratio of the resin column is 1:1, followed by 1BV of purified water at 4BV / h The resin column was cleaned at a flow rate of h, and then eluted with 1BV of 70% ethanol at a flow rate of 4BV / h, the eluate was collected, and the eluate was concentrated under reduced pressure to no alcohol. 0.07MPa, recover etha...

Embodiment 2

[0023] 100 kg of fenugreek seeds were pressed with a screw oil press at a pressing temperature of 25° C. and a pressing pressure of 25 MPa to remove lipid components to obtain fenugreek seed cakes. The fenugreek seed cake was leached with 7 times of purified water, the leaching temperature was 35°C, and the leaching time was 2 hours, the extract was filtered to obtain the filtrate, and the purified water was leached again in the above-mentioned manner, and the filtrates were combined. Purify the combined filtrate with a CAD-40 macroporous adsorption resin column at a flow rate of 0.5BV / h, wherein the amount of resin is 6 times the amount of crude drug, and the diameter-to-height ratio of the resin column is 1:5, followed by 2BV of purified water with 2BV Wash the resin column at a flow rate of 1.5BV / h, then elute with 1.5BV of 70% ethanol at a flow rate of 2BV / h, collect the eluate, and concentrate the eluent under reduced pressure to no alcohol. -0.08MPa, recover ethanol, spr...

Embodiment 3

[0025] 100 kg of fenugreek seeds were pressed with a screw oil press at a pressing temperature of 40° C. and a pressing pressure of 10 MPa to remove lipid components to obtain fenugreek seed cakes. The fenugreek seed cake was leached with 8 times of purified water, the leaching temperature was 20°C, and the leaching time was 2 hours, the extract was filtered to obtain the filtrate, and the purified water was leached again in the above-mentioned manner, and the filtrates were combined. Purify the combined filtrate with a CAD-40 macroporous adsorption resin column at a flow rate of 2BV / h, wherein the amount of resin is 4 times the amount of crude drug, and the ratio of diameter to height of the resin column is 1:3, followed by 3BV of purified water at 3BV / h The resin column is washed at a flow rate of h, and then eluted with 2BV of 70% ethanol at a flow rate of 3BV / h, the eluate is collected, and the eluate is concentrated under reduced pressure to no alcohol. 0.09MPa, recover e...

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Abstract

The invention discloses a method for extracting protodioscin from fenugreek seeds. The method comprises the following the steps: (1) pressing the fenugreek seeds with a screw-type oil expeller; taking off lipid compositions to obtain fenugreek seed cake meal; (2) extracting the fenugreek seed cake meal with purified water twice, filtering the extracting solution to obtain filtrates; combining the filtrates; (3) conducting purification on the combined filtrate with a CAD-40 macroporous resin column, carrying out washing with water, using ethyl alcohol with a concentration of 70% for elution, carrying out decompressing and concentrating till no alcohol exists, and carrying out spraying and drying to obtain an extraction containing protodioscin. The method for extracting protodioscin from the fenugreek seeds is simple in extracting operation, and low in energy consumption and cost, and has the advantage that the purity of effective components in the extraction can be improved.

Description

technical field [0001] The invention relates to a method for extracting protodioscin, in particular to a method for extracting protodioscin from fenugreek seeds. Background technique [0002] Protodioscin belongs to steroidal saponins. Protodioscin has the functions of enhancing sexual function, lowering blood fat, poisonously killing cancer cells and anti-leukemia. [0003] Patent application document CN 102670697 A discloses a preparation method of fenugreek water extract, comprising the following steps: (1) take fenugreek seeds, add 10 times the weight of water, soak for 20-30 minutes, boil for 20-30 minutes, Take out and keep the filtrate; (2) add 8 times the amount of water to the filter residue, soak for 20-30 minutes, boil for 20-30 minutes, and keep the filtrate; (3) combine the two filtrates, dry them in a water bath to obtain a gourd Ba water extract. [0004] The content of protodioscin in the aqueous extract of fenugreek prepared above is low, the yield is low,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61P15/00A61P3/06A61P35/00A61P35/02
Inventor 郑猛马全民聂复礼杨浩王青青
Owner ZHEJIANG HUISONG PHARMA
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