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Quantitative analysis method of six chemical components in Chinese herbal medicine compound preparation using rhizoma dioscoreae nipponicae and acanthopanax roots as Chinese herbal medicines

A technology for compound preparations and chemical components is applied in the field of quantitative analysis of 6 chemical components in traditional Chinese medicine compound preparations, which can solve the problems of difficult quality control, interference of target peaks, and large fluctuations in the baseline of chromatographic peaks, saving detection time and detection. cost, improved quality control, simple and fast operation

Active Publication Date: 2015-04-08
TIANJIN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As we all know, Chinese medicine compound preparations use multiple active ingredients to exert their medicinal effects. The existing quality control standards mostly use the content of a single ingredient as an index to evaluate the quality of Chinese medicine, which is difficult to reflect the transfer of various active ingredients in the production process. difficult to control
Diosgenin, protodioscin and pseudoprotodioscin belong to steroidal saponins, and their ultraviolet absorption belongs to terminal absorption. Complexity, using a diode array detector (DAD) detection method cannot achieve baseline separation of target peaks and impurity peaks
However, isoxazinidine, syringin and chlorogenic acid cannot be effectively separated by evaporative light scattering detector (ELSD) due to their non-selective structure, and the response is very small.
[0003] At present, there is no literature report on simultaneous quantitative determination of steroidal saponins, organic acids and flavonoids

Method used

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  • Quantitative analysis method of six chemical components in Chinese herbal medicine compound preparation using rhizoma dioscoreae nipponicae and acanthopanax roots as Chinese herbal medicines
  • Quantitative analysis method of six chemical components in Chinese herbal medicine compound preparation using rhizoma dioscoreae nipponicae and acanthopanax roots as Chinese herbal medicines
  • Quantitative analysis method of six chemical components in Chinese herbal medicine compound preparation using rhizoma dioscoreae nipponicae and acanthopanax roots as Chinese herbal medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The quantitative analysis method of 6 chemical constituents in the traditional Chinese medicine compound capsules with Chuanshanlong and Acanthopanax as medicinal materials comprises the following steps:

[0035] (1) Prepare a mixed reference solution: take an appropriate amount of dioscin, pseudoprotodioscin, protodioscin, chlorogenic acid, syringin and isoxazinidine reference substance, weigh them accurately, and add methanol to prepare 145 μg per 1 mL A mixed reference solution of diosgenin, 231 μg pseudoprotodioscin, 245 μg protodioscin, 81 μg chlorogenic acid, 57 μg syringin and 95 μg isozaperidine;

[0036] (2) Preparation of the first test solution: take the traditional Chinese medicine compound capsules made of Chuanshanlong and Acanthopanax as medicinal materials, remove the capsule shell, grind finely, pass through a No. 4 sieve, take about 1.0g, accurately weigh, set Add 10mL of methanol aqueous solution with a volume concentration of 85% to the stoppered Erl...

Embodiment 2

[0039] The quantitative analysis method of 6 chemical constituents in the traditional Chinese medicine compound sugar-coated tablet with Chuanshanlong and Acanthopanax as medicinal materials comprises the following steps:

[0040] (1) Preparation of mixed reference substance solution: with step (1) of Example 1;

[0041] (2) Preparation of the first test solution: take the Chinese medicine compound sugar-coated tablet made of Pangosaurus and Acanthopanax as medicinal materials, remove the sugar coating, grind it finely, pass through a No. 4 sieve, take about 1.0g, accurately weigh, set In a conical flask with a stopper, add 10 mL of methanol aqueous solution with a volume concentration of 70% precisely, weigh it, and ultrasonically treat it for 55 minutes under the conditions of a power of 120 W and a frequency of 40 kHz, let it cool to room temperature, and then weigh it again, using a volume concentration of 70% aqueous methanol solution to make up the lost weight, shake wel...

Embodiment 3

[0044] Quantitative analysis method for 6 chemical components in traditional Chinese medicine compound granules with Chuanshanlong and Acanthopanax as medicinal materials, including the following steps:

[0045] (1) Preparation of mixed reference substance solution: with step (1) of Example 1;

[0046] (2) Preparation of the first test solution: take the traditional Chinese medicine compound preparation granules made of Chuanshanlong and Acanthopanax as medicinal materials, grind it finely, pass through a No. 4 sieve, take about 1.0g, accurately weigh it, and put it in the shape of a plug. Into the bottle, add 10 mL of methanol aqueous solution with a volume concentration of 90% precisely, weigh it, and ultrasonically treat it for 60 minutes under the conditions of power 120W and frequency 40kHz, let it cool to room temperature, weigh it again, and use methanol with a volume concentration of 90%. The aqueous solution makes up the lost weight, shakes well, filters, and takes th...

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Abstract

The invention discloses a quantitative analysis method of six chemical components in a Chinese herbal medicine compound preparation using rhizoma dioscoreae nipponicae and acanthopanax roots as Chinese herbal medicines. The quantitative analysis method comprises the following steps: (1) preparing a mixed control solution; (2) preparing a first test sample solution and a second test sample solution; (3) determining contents, namely, respectively taking the mixed control solution, the first test sample solution and the second test sample solution, performing gradient elution under high performance liquid chromatography detection conditions of using C18 bonded silica gel as a chromatographic column of a filling agent and acetonitrile-formic acid solution as a mobile phase, performing high performance liquid chromatography detection by combining a diode array detector and an evaporative light-scattering detector of detectors to obtain high performance liquid chromatograms of the mixed control solution, the first test sample solution and the second test sample solution, and determining the contents of dioscin, pseudoprotodioscin, protodioscin, chlorogenic acid, syringin and isofraxidin in a test sample by using an external standard method. The quantitative analysis method is simple, convenient and rapid to operate, high in accuracy and excellent in repeatability, and can be used for improving the quality control level of a product.

Description

technical field [0001] The invention relates to a quantitative analysis method for six chemical components in a compound Chinese medicine preparation. Background technique [0002] The main medicinal ingredients of the traditional Chinese medicine compound preparations with Chuanshanlong and Acanthopanax as medicinal materials include diosgenin (diosgenin), trillium, methyldioscin, protodioscin, pseudoprotodioscin, syringin (acanthopanax Adding glycoside B), eleutheroside E, isoxazinidine, quercetin, chlorogenic acid, syringin, etc. As we all know, Chinese medicine compound preparations use multiple active ingredients to exert their medicinal effects. The existing quality control standards mostly use the content of a single ingredient as an index to evaluate the quality of Chinese medicine, which is difficult to reflect the transfer of various active ingredients in the production process. Difficult to control. Diosgenin, protodioscin and pseudoprotodioscin belong to steroi...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 蒋建兰任为之孙秀婷王顺仙
Owner TIANJIN UNIV
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