Method for comprehensively extracting protodioscin and dioscin from dioscorea nipponica

A protodioscin, comprehensive extraction technology, applied in chemical instruments and methods, preparation of steroids, sugar derivatives, etc., to achieve the effect of shortening the process flow, optimizing extraction, and better effect

Active Publication Date: 2013-01-16
SHAANXI JIAHE PHYTOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The present invention aims at the comprehensive development of the raw material of Chuandilong, and provides a method for comprehensively extracting protodioscin and diosgenin from the raw material of Chuan

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) extract

[0042] Grinding the raw material of Diodontia to obtain a pulverized product, extracting the pulverized product with methanol in an amount 3 times the mass of the raw material of Chuandilong, the extraction temperature is 95°C, and the extraction time is 2h, 3 times; the extract is obtained.

[0043] (2) concentrated

[0044] Concentrate the obtained extract to obtain an extract with a specific gravity of 1.05; then filter to obtain a filtrate and a precipitate.

[0045] (3) crystallization

[0046] Dissolve the precipitate obtained in step (2) with 2 times the amount of 95% ethanol, filter at a temperature of 35°C, add acetone of 1 / 3 of the volume of the filtrate to the filtrate, place it in an ice bath to crystallize, centrifuge, and dry in vacuum to obtain a white powder. The powder is the dioscin product. The content of the obtained diosgenin was 93.86%, and the recovery rate of the active ingredient was 91%.

[0047] (4) Extraction

[0048] The fil...

Embodiment 2

[0052] (1) extract

[0053] Grinding the raw material of Diortonia chinensis to obtain a pulverized product, and extracting the pulverized product with acetone 5 times the mass of the raw material of Diodranillium, the extraction temperature is 50°C, and the extraction time is 2 hours, twice; to obtain an extract.

[0054] (2) concentrated

[0055] Concentrate the obtained extract to obtain an extract with a specific gravity of 1.15; then filter to obtain a filtrate and a precipitate.

[0056] (3) crystallization

[0057] Dissolve the precipitate obtained in step (2) with 8 times the amount of 95% ethanol, filter at a temperature of 50°C, add chloroform of 1 / 8 of the volume of the filtrate to the filtrate, place it in an ice bath to crystallize, centrifuge, and dry in vacuum to obtain a white powder. The powder is the dioscin product. The obtained diosgenin content was 92.22%, and the recovery rate of active ingredients was 90%.

[0058] (4) Extraction

[0059] The filtra...

Embodiment 3

[0063] (1) extract

[0064] Grind the raw material of Diortonia chinensis to obtain a pulverized product, and extract the pulverized product with 80% ethanol which is 7 times the mass of the raw material of Diodanera, the extraction temperature is 75°C, and the extraction time is 2 hours, twice; to obtain the extract.

[0065] (2) concentrated

[0066] Concentrate the obtained extract to obtain an extract with a specific gravity of 1.12; then filter to obtain a filtrate and a precipitate.

[0067] (3) crystallization

[0068] Dissolve the precipitate obtained in step (2) with 4 times the amount of 95% ethanol, filter at a temperature of 75°C, add acetone of 1 / 5 of the volume of the filtrate to the filtrate, place it in an ice bath to crystallize, centrifuge, and dry in vacuum to obtain a white powder. The powder is the dioscin product. The obtained diosgenin content was 97.54%, and the recovery rate of active ingredients was 92%.

[0069] (4) Extraction

[0070] The filtr...

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Abstract

The invention aims to compressively develop dioscorea nipponica raw materials, and provides a method for comprehensively extracting protodioscin and dioscin from dioscorea nipponica. The technical concept is shown by the following steps: 1, crushing raw materials, synchronously extracting protodioscin and dioscin through an organic solvent; 2, combining the extracting liquid, concentrating to reach proportion of about 1.12, filtering, dissolving the obtained precipitate through an ethanol solution, then separating dioscin from the precipitate (preferably utilizing acetone), and filtering to obtain a dioscin product; and 3, extracting the mother liquor filtered in step 2, concentrating and parching the extracting liquid to obtain a rough product, and recrystallizing the rough product to obtain the protodioscin product. With the adoption of the method provided by the invention, the problem in the prior art that the resource is wasted because two products cannot be comprehensively developed can be overcome, the extracting step is optimized, the producing cost is reduced, and the operability is improved.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine chemistry, and relates to a method for extracting protodioscin and diosgenin, in particular to a method for comprehensively extracting protodioscin and diosgenin from the raw material of Diodes longum. Background technique [0002] Chuandilong, also known as Chuanshanlong, is the rhizome of the Dioscorea plant Dioscorea piercosa, a perennial herb. Flat in nature and bitter in taste, it has the functions of promoting blood circulation and relaxing tendons, promoting digestion and diuresis, eliminating phlegm and reducing malaria. Control wind-cold-damp arthralgia, chronic bronchitis, indigestion, strain and sprain, malaria, carbuncle and other effects. Folks use it to treat numbness of muscles and bones, backache and leg pain. Modern studies have proved that Dilonga has various pharmacological effects such as regulating immunity, improving cardiovascular function, antitussive...

Claims

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Application Information

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IPC IPC(8): C07J71/00C07H17/04C07H1/08
Inventor 肖红惠玉虎王春德王晓莹
Owner SHAANXI JIAHE PHYTOCHEM
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