Method for extracting protodioscin from dioscorea opposita plant

A protodioscin and plant technology, which is applied in the field of extracting high-purity protodioscin, can solve the problems of hydrolysis interference, large water consumption, and large waste water discharge, so as to improve purity and yield, improve efficiency, and reduce residues Effect

Active Publication Date: 2016-07-27
HUNAN AGRICULTURAL UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] The process of producing protodioscin by direct acid hydrolysis process mainly has the following problems: the first is that the existence of a large amount of cellulose and starch interferes with the hydrolysis, so the yield of protodioscin is low; the other is that the raw material is

Method used

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Embodiment 1

[0027] The raw material of this embodiment adopts Dioscorea chinensis. A method for extracting protodioscin from yam plants, comprising the following steps: crushing the raw material yam yam to 15 mesh, adding water three times the weight of the dry weight of the raw material and a compound enzyme of 1.5% of the dry weight of the raw material to carry out enzymatic hydrolysis for 120 minutes; The compound enzyme is composed of cellulase and amylase in a weight ratio of 3:1; after enzymolysis, filter, take the filter residue to granulate, and dry at 50°C for 2.5h after granulation; then perform supercritical extraction to keep CO 2 The flow rate is 25l / h, the pressure is 20MPa, and the extraction temperature is 55°C; after 1.5h of extraction, it is placed in a separation tank for separation, the separation pressure is 15MPa, and the separation temperature is 45°C, and the product of protodioscin is obtained by separation. After testing, the obtained protodioscin has a purity of...

Embodiment 2

[0029] The raw material of this embodiment adopts Dioscorea scutellariae. A method for extracting protodioscin from yam plants, comprising the following steps: crushing the raw material yam dioscens to 20 mesh, adding water twice the weight of the dry weight of the raw material and a compound enzyme of 1% of the dry weight of the raw material to carry out enzymatic hydrolysis for 90 minutes; The compound enzyme is composed of cellulase and amylase in a weight ratio of 3:1; after enzymolysis, filter, take the filter residue to granulate, and dry at 40°C for 2 hours after granulation; then perform supercritical extraction to keep CO 2 The flow rate is 15l / h, the pressure is 15MPa, and the extraction temperature is 50°C; after extraction for 1h, it is put into a separation tank for separation, the separation pressure is 10MPa, and the separation temperature is 35°C, and the protodioscin product is obtained by separation. After testing, the obtained protodioscin has a purity of 98...

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Abstract

The invention relates to a method for extracting protodioscin from a dioscorea opposita plant, in particular to a method for extracting high-purity protodioscin by adopting a compound enzyme of cellulase and amylase to perform pretreatment and utilizing the characteristics of CO2 at a supercritical state. The method has the advantages of simple technological flow, high extraction efficiency, complete active ingredients and no environmental pollution.

Description

technical field [0001] The invention relates to a method for extracting protodioscin from yam plants, in particular to a method for pretreatment by enzymatic method, and then using CO 2 A method for the characteristic extraction of high-purity protodioscin in a supercritical state. Background technique [0002] Dioscorea plant has pharmacological activities such as anti-inflammatory, expectorant, antiasthma, increase coronary flow, lower blood lipid, improve cardiovascular function, antibacterial, antiviral, etc. It can also slow down heart rate, enhance myocardial contractility, increase daily urine output, improve Coronary circulation, lower arterial blood pressure, especially for mild arteriosclerosis. Protodioscin can inhibit the synthesis of DNA, RNA and protein and cell growth of human leukocyte HL-60. Another study found that leukemia, colon cancer and prostate cancer cell lines are most sensitive to protodioscin. According to the COMPARE degree analysis, in the Na...

Claims

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Application Information

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IPC IPC(8): C07J71/00
CPCC07J71/0005Y02P20/54
Inventor 林海燕刘仲华张志旭李勤谭斌崔江涛
Owner HUNAN AGRICULTURAL UNIV
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