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Ginseng saponin extract and preparation method thereof

A technology of ginsenosides and total ginsenosides, which is applied in the direction of testing pharmaceutical preparations, drug combinations, and pharmaceutical formulations, and can solve the problems of many by-products, low yield of active ingredients, and poor separation of ginsenoside H.

Inactive Publication Date: 2013-12-04
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The effect of separating ginsenoside H in the prior art is not good, the yield of active ingredients is low, and there are many by-products, so it is difficult to industrialize production

Method used

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  • Ginseng saponin extract and preparation method thereof
  • Ginseng saponin extract and preparation method thereof
  • Ginseng saponin extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] The preparation of embodiment 1 ginsenoside H extract

[0092] Step 1. American ginseng leaves were extracted with water twice, each time for 2 hours, adding 10 times the amount of water each time. After the extract was concentrated, it passed through a D101 macroporous adsorption resin column, and was first eluted with water until the effluent was nearly colorless, and then used 85% ethanol was eluted, and the 85% ethanol eluate was collected and concentrated to dryness to obtain total saponins;

[0093] Step 2. Dissolve 10kg of total saponins and 10kg of sodium hydroxide in 60kg of glycerin, heat, control the reaction temperature at 230°C, and the reaction time is 30min to obtain the alkali degradation product.

[0094] Step 3, the alkali degradation product was dissolved by heating with 95% ethanol, mixed with 2 times the amount of silica gel, dried, crushed and sieved, and subjected to silica gel column chromatography. The ratio of alkali degradation product to chro...

Embodiment 2

[0095] The preparation of embodiment 2 ginsenoside H extract

[0096] Step 1. American ginseng leaves were extracted with water for 3 times, each time for 3 hours, adding 10 times of water each time, the extract was passed through the D101 macroporous adsorption resin column, firstly eluted with water until the effluent was nearly colorless, and then used 50% Eluted with ethanol, collected the eluate, concentrated to dryness to obtain total saponins;

[0097] Step 2. Dissolve 10 kg of total saponins and 10 kg of potassium hydroxide in 60 kg of glycerol, heat, and control the reaction temperature at 150° C. for 60 minutes. Alkaline degradation products are obtained,

[0098] Step 3, the alkali degradation product was dissolved by heating with 85% ethanol, mixed with 3 times the amount of silica gel, dried, crushed and sieved, and subjected to silica gel column chromatography. The ratio of alkali degradation product to chromatography silica gel was 1:5. Elute with a solution s...

Embodiment 3

[0099] The preparation of embodiment 3 ginsenoside H extract

[0100] Step 1. American ginseng is extracted with water, and the extract is passed through an AB-8 macroporous adsorption resin column, and firstly eluted with water until the effluent is nearly colorless, followed by elution with 95% ethanol, and the 95% ethanol eluate is collected. Concentrate to dryness to obtain total saponins;

[0101] Step 2. Dissolve 10 kg of total saponins and 10 kg of sodium carbonate in 60 kg of glycerin, heat, and control the reaction temperature at 200° C. for 20 minutes. Alkaline degradation products are obtained,

[0102] Step 3, the alkali degradation product is dissolved by heating with 95% ethanol, mixed with 2 times the amount of silica gel, dried, crushed and sieved, and subjected to silica gel column chromatography. The ratio of alkali degradation product to chromatographic silica gel is 1:10. The solution system with a volume ratio of chloroform-methanol (8:1) was used for el...

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Abstract

The invention relates to a traditional Chinese medicine extract and a preparation method thereof, in particular to a Ginseng saponin extract and a preparation method thereof. In the extract, the content of protodioscin is not less than 80 percent based on that of Ginseng saponin Rh2, the content of Ginseng saponin Rh1 and the Ginseng saponin Rh2 is not less than 50 percent, the content of Ginseng saponin Rh1 is between 1 and 20 percent and the content of the Ginseng saponin Rh2 is between 20 and 60 percent.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract and its preparation, in particular to a ginsenoside extract and its preparation. Background technique [0002] Ginsenoside H is the secondary saponin produced by alkali-catalyzed hydrolysis of ginsenoside components, and then purified by silica gel column chromatography to obtain the effective part-H group saponin. Its main components are ginsenosides Rh1, Rh2 and Rh3, of which Rh2 is the main component of the drug and also the main anticancer active component of the drug. Pharmacodynamic studies have shown that: ginsenoside Rh2 has a significant and specific inhibitory effect on the proliferation of cancer cells such as liver cancer, melanoma B16, cervical cancer and sarcoma S180. Its mechanism of action is as follows: in the process of cancer cell proliferation, through the action of ginsenoside Rh2, the cancer cells stop at a certain proliferation stage and promote their reversion to n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/704A61K36/258A61K9/20G01N31/22G01N30/90G01N30/02G01N33/15G01N21/31A61P35/00A61K125/00A61K127/00
Inventor 宋兆辉张兰兰黄芝娟林万里蔡楠周水平朱永宏
Owner TIANJIN TASLY PHARMA CO LTD
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