Method for extracting isolate anti-BACE-1 enzyme from urechis unicinctus

A technology of BACE-1 and single-ring thorn, applied in drug combinations, steroids, nervous system diseases, etc., can solve the problem that there is no new breakthrough in the treatment of Alzheimer's disease

Inactive Publication Date: 2018-04-06
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no drug that can completely prevent or cure Alzheimer's di

Method used

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Experimental program
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Effect test

Embodiment 1

[0022] Kelp, sargassum, sea cabbage and C. monocircleum were extracted with organic solvents, concentrated to prepare dry extracts, and the anti-BACE-1 enzyme activity of each extract was determined. It was found that the C. monocycle extract The anti-BACE-1 enzyme activity of the extract was the strongest, and when the concentration of the extract was 2.0 mg / mL, the inhibition rate of BACE-1 enzyme was 67.24%.

Embodiment 2

[0024] The results of extraction, separation and activity determination of the ethanol extract of C. monoringensis: Extraction and separation of the ethanol extract of C. monoringensis with extractant A, extractant B, extractant C and extractant D in sequence, and the collected extracts were rotated and evaporated. Obtain each extract phase and water phase dry matter, carry out anti-BACE-1 enzyme activity measurement to each extract phase and water phase, the result finds, the isolate of extractant B has the strongest anti-BACE-1 enzyme activity, when the extract concentration is 2.0 mg / mL, the inhibition rate of BACE-1 enzyme was 79.8%.

Embodiment 3

[0026] Separation of the extract obtained by extractant B and determination of anti-BACE-1 enzyme activity: Weigh a certain amount of extractant B to obtain the extract, and use chloroform / methanol in different volume ratios as eluents to test The methane extract was separated by silica gel column chromatography to obtain components B1~B7, and the anti-BACE-1 enzyme activity of each component was determined. It was found that the B5 component and B6 component had the strongest inhibitory effect on the BACE-1 enzyme activity. When the concentration of the isolate was 2.0 mg / mL, the inhibition rate of B5 fraction to BACE-1 enzyme was 94.15%, and the inhibition rate of B6 fraction to BACE-1 enzyme was 100.00%.

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Abstract

The invention discloses a method for extracting an isolate anti-BACE-1 enzyme from urechis unicinctus. The method is characterized by comprising the steps of primary screening of marine-derived activesubstances and determination of anti-BACE-1 enzyme activity; isolation of anti-BACE-1 enzyme active substances B5 and B6 in ethanol extract of urechis unicinctus and single compound structure identification; and determination of anti-BACE-1 enzyme activity by fluorescence method, and selection of curcumin as an activity reference substance. The single compounds PS-1 and PS-2 with high anti-BACE-1enzyme activity are prepared by the method, wherein the PS-1 is 4-cholestene-3-acetone and the PS-2 is hecogenin. The method disclosed by the invention has strong anti-BACE-1 enzyme activity, and theseparation method is simple and feasible, and provides a basis for extraction, separation and activity research of the active ingredients in the urechis unicinctus.

Description

technical field [0001] The invention belongs to the technical field of application of marine plants, and more specifically relates to a method for extracting and resisting BACE-1 enzyme of isolates from Echinopsis monocircleum. Background technique [0002] Urchis unicinctus, commonly known as sea intestine, is a true body coelom protostome, belonging to the phylum Echiuroidea, class Echiurida, order Xenopneusta, family Urechidae in classification , the genus Urechis, is the only species of the Anthracite order distributed along the coast of China. C. monoringus is mainly distributed in Russia, Japan, North Korea and the coasts of the Yellow Sea and Bohai Sea in my country. It is an economical marine invertebrate that has gradually attracted people's attention and understanding in recent years. Because of its unique classification status and its ability to tolerate high concentrations of sulfides, it has always been one of the hotspots of academic research. [0003] In rec...

Claims

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Application Information

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IPC IPC(8): C07J9/00C07J71/00A61P25/28
CPCC07J9/00C07J71/0005
Inventor 朱永哲薛长晖刘静文
Owner QINGDAO AGRI UNIV
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