Genetically engineered bacteria and its application in the preparation of 9α,22-dihydroxy-23,24-bisnorcholest-4-en-3-one

Abstract

The invention relates to the technical field of genetic engineering, in particular to mycobacterium genetically engineered bacteria and its application in the preparation of 9α,22-dihydroxy-23,24-bisnorcholester-4-en-3-one. The genetically engineered bacterium is three 3-sterone-∆ in mycobacteria 1 ‑Dehydrogenase gene kstd 1, kstd 2 and kstd 3 inactivated, simultaneously overexpressed gene constructs encoding acetyl-CoA acetyltransferase / thiolase and DNA-binding protein, which can selectively produce 9α, 22-dihydroxy-23, 24-dinorcholesterol-4 ‑ene‑3‑ketone reduces the generation of by-product 9‑OH‑AD, greatly improves production efficiency and product quality, makes the product easy to separate and purify, reduces production cost, and reduces environmental pollution at the same time.

Description

technical field

[0001] The invention relates to the technical field of genetic engineering, in particular to a mycobacterium genetically engineered bacterium and its application in the preparation of 9α, 22-dihydroxy-23, 24-bisnorcholest-4-en-3-one. Background technique

[0002] Steroids are a class of compounds with a cyclopentane polyhydrophenanthrene ring structure, usually with methyl groups at C-10 and C-13 and alkyl side chains at C-17. Steroids, as a component of cell membranes, play an important role in living organisms. Some steroids also act as hormones and signaling molecules. Since the discovery of steroid drugs in the 1950s, more than 300 steroid drugs have been identified so far. Steroidal drugs have strong pharmacological effects such as anti-infection, anti-allergy, anti-virus and anti-shock. In recent years, the application range of steroid drugs in the medical field has been continuously expanded, and they are widely used in the treatment of rheumatism, ...

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