Process for biologically synthesizing extracellular cholesterol oxidase from Brevibacterium and its transferred product cholest-4-ene-3-one

A technology of cholesterol oxidase and Brevibacterium, applied in the direction of oxidoreductase, fermentation, etc., can solve the problems that have not been reported in the public literature, so as to reduce the absorption of cholesterol, increase high-density lipoprotein, and reduce low-density lipoprotein. Effect
CN1208454CInactive Publication Date: 2005-06-29JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2005-06-29
Estimated Expiration
Not applicable · inactive patent

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Abstract

A process for biologically synthesizing the extracellular cholesterol oxidase from Brevibacterium is disclosed. The high-output bacterium DGCDC-82 is fermented in optimized condition to obtain said enzyme up to 700-1000 U / L. After purified, its specific enzyme activity can reach 30 U / mg. Said enzyme contains coenzyme FAD and cysteine in the active center of enzyme. In the dual-phase (water phase and organic phase) system, said enzyme can be used to uniquely oxidize the cholesterol to becone cholest-4-ene-3-ketone with 92% of transfer rate, which can be used to reduce cholesterol, blood fat and low-density lipoprotein, so having wide application range.
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Description

technical field

[0001] The present invention relates to the biosynthesis of extracellular cholesterol oxidase by self-selected Brevibacterium sp. A method for enzymatically catalyzing the oxidation of cholesterol to produce a single oxidation product, cholest-4-en-3-one. The toxicity and blood lipid lowering of cholest-4-en-3-one were studied, indicating its application in food and medicine. The invention belongs to the technical field of microbial enzyme engineering. Background technique

[0002] Cholesterol is an indispensable organic component in the human body, and the concentration of cholesterol in the blood is an important physiological indicator that can reflect the health status of the human body. Excessive cholesterol content in food will also have a negative impact on people's health. It is precisely because of the discovery of cholesterol oxidase in microbial cells that there are a series of fast, simple and automatic enzymatic methods for determining the conc...

Claims

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