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Uses of 5-cholesten-3β-ol sulfate

A technology of alcohol sulfate and cholestene, applied in the field of 5-cholestene-3β-alcohol sulfate salt, can solve the problems such as unpublished treatment effect of type I diabetes mellitus

Active Publication Date: 2021-12-24
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the effect of 5-cholesten-3β-ol sulfate on islet β cells and the therapeutic effect on type I diabetes have not been disclosed yet.

Method used

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  • Uses of 5-cholesten-3β-ol sulfate
  • Uses of 5-cholesten-3β-ol sulfate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1、5

[0022] The preparation of embodiment 1,5-cholestene-3β-alcohol sulfate injection

[0023] Dissolve CS in DMSO to prepare a 50 mg / mL solution, then add co-solvent Tween 20, and finally dissolve the above mixture in normal saline to prepare CS injection. Among them, the concentration of CS is 5mg / L, DMSO is 10%, and Tween 20 is 2%, which is prepared and used now.

[0024] The beneficial effects of the present invention are demonstrated through experimental examples below.

experiment example 1、5

[0025] Experimental example 1, the inhibitory effect of 5-cholesten-3β-ol sulfate on the hyperglycemia of type I diabetes induced by STZ

[0026] 1. Experimental method

[0027] The C57 / 6J mice were divided into 4 groups, namely vehicle group, CS group, STZ group and CS+STZ group.

[0028] (1) Preparation of solution:

[0029] Preparation of DMSO injection: Dissolve DMSO and Tween 20 in normal saline to make an injection with 10% DMSO and 2% Tween 20, ready for immediate use.

[0030] Preparation of STZ citric acid buffer solution: Add 2.1g of citric acid to 100mL of double distilled water to make solution A (0.1mol / L), add 2.94g of sodium citrate to 100mL of double distilled water to make solution B (0.1mol / L) . Mix liquid A and liquid B at a ratio of 1:1.32, measure the pH value with a pH meter, adjust the pH=4.2-4.5, and finally dissolve STZ in the above mixed liquid to prepare STZ citric acid injection with a concentration of 8 mg / L. Ready to use

[0031] (2) Mouse pr...

experiment example 2、5

[0043] Experimental Example 2, Effect of 5-cholesten-3β-ol sulfate on pancreatic islet β cells

[0044] 1. STZ treatment of NIT-1 flow cytometry apoptosis experiment:

[0045] NIT-1 cells were plated overnight and pretreated with corresponding concentrations of CS solution (0 μM, 5 μM, 10 μM, DMSO as solvent) for 6 hours, and then added with corresponding concentrations of STZ solution (10 mM, 20 mM) and CS (0 μM, 5 μM, 10 μM , DMSO as solvent) for 24 hours. Apoptosis detection kit (Cat: FXP023) of Beijing Sizhengbai Biotechnology Company was used to detect cell apoptosis.

[0046] 2. Edu proliferation experiment:

[0047] NIT-1 cells were plated overnight and then treated with corresponding concentrations of CS (0 μM, 10 μM, 20 μM, DMSO as solvent) for 24 hours, and the cell proliferation was detected with Ruibo Edu detection kit (Cat: C10320-1).

[0048] 3. Insulin and glucagon staining experiment:

[0049] The fresh pancreatic tissues of the mice in each experimental gr...

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Abstract

The invention provides the use of 5-cholestene-3β-ol sulfate in the preparation of drugs for reducing the apoptosis of pancreatic beta cells, promoting the proliferation of pancreatic beta cells, and protecting the morphology and structure of pancreatic islets, and the preparation of drugs containing 5-cholestene-3β-ol Alcohol Sulfate Drugs. 5-Cholesterene-3β-ol sulfate can reduce the apoptosis of islet β cells, promote the proliferation of islet β cells, protect the morphology and structure of islets, prevent the loss of islet β cells, enhance insulin secretion, and significantly improve the symptoms caused by insulin reduction. Hyperglycemia, reversing type I diabetes, provides a new option for clinic.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the application of 5-cholestene-3β-alcohol sulfate. Background technique [0002] Type I diabetes, also known as insulin-dependent diabetes, accounts for about 10% of the total number of diabetic patients. Most of them develop quickly and have significant and severe symptoms. At present, it is believed that the most important reason for the occurrence of type I diabetes is autoimmunity mediated by autoreactive T lymphocytes, which induces the autoimmune response of pancreatic β cells, causes damage and apoptosis of pancreatic β cells, and damages pancreatic β cells to make them Loss of the function of synthesizing and secreting insulin, causing glucose metabolism disorder. [0003] Early treatment of type 1 diabetes used immunosuppressants (such as cyclosporine) to block the autoimmune process and preserve the function of islet β cells, but long-term use would cause adverse...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/575A61K9/08A61K47/26A61P3/10A61P5/50
CPCA61K31/575A61K9/08A61K47/26A61P3/10A61P5/50
Inventor 苏智广刘银张雪萍张彩霞
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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