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Application of black raspberry extract in preparing anti-diabetic medicine

An anti-diabetic and extract technology, applied in the field of medicine, can solve the problems such as no report of black raspberry or its extract, insulin receptor substrate defect, insulin signal transduction defect, etc., to promote the proliferation of pancreatic islet cells, improve the Elevated blood sugar level, effect of hypoglycemic effect

Active Publication Date: 2013-06-12
赵红石
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(2) Insulin receptor substrate deficiency
(3) Insulin signal transduction defect
[0005] In the prior art, there is no relevant report of black raspberry or its extract in the treatment of diabetes

Method used

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  • Application of black raspberry extract in preparing anti-diabetic medicine
  • Application of black raspberry extract in preparing anti-diabetic medicine
  • Application of black raspberry extract in preparing anti-diabetic medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Preparation and component analysis of raspberry ripe fruit extract (BRBFE) and raspberry leaf extract (BRBlE)

[0017] This example provides two methods for preparing ripe raspberry fruit extract.

[0018] (1) Ripe raspberry fruit (black raspberry or red raspberry) is mashed by a masher, then passed through a 0.02-inch aperture sieve, filtered to remove seeds, and made into puree. Strain the remaining fruit and repeat this operation a further three times to ensure adequate juice extraction. Juicing rate is 97%. The prepared puree was poured into a 1-inch freeze-drying dish coated with polyethylene film, and stored in a ventilated freezer. Further freeze-drying is carried out by a sublimator to obtain black raspberry fruit freeze-dried powder (or red raspberry fruit freeze-dried powder). Add the black raspberry fruit freeze-dried powder (or red raspberry fruit freeze-dried powder) into ethanol: water (80:20) solvent, soak and extract, temperature 25 ℃; ti...

Embodiment 2

[0025] Example 2 : Effect of Raspberry Extract on Promoting the Growth and Differentiation of Rat Islet-like Cells in Vitro

[0026] The rat islet-like (INS-1) cell line was selected, and the activity of raspberry extracts (BRBFE, BRBLE, RRBFE, RRBLE) in promoting cell proliferation in vitro was determined by MTT method. The measured concentration was 0-100 μg / ml, and the treatment time was for 72 hours. BRBFE and BRBLE can obviously promote the growth and differentiation of pancreatic islets. T-test for statistical analysis, p<0.05 has a significant difference, the results are shown in Table 1.

[0027]

[0028] The results showed that the black raspberry extracts (BRBFE and BRBLE) could significantly promote the proliferation of islet-like cells INS-1, and the effect was significantly better than the red raspberry extracts (RRBFE and RRBLE) in the study. Compared with blank control group, **p<0.01, *p<0.05.

Embodiment 3

[0029] Example 3: Inhibitory effect of raspberry extract on PTP1B enzyme activity

[0030] Protein tyrosine phosphatase (PTP1B) can hydrolyze the phosphoester bond of the specific substrate p-nitrophenol phosphate disodium (pNPP), and a color reaction occurs, and the obtained product p-nitrophenol sodium has a strong intensity at 410nm. Light absorption, the change of light absorption at 410nm is directly detected by a microplate reader to observe the change of enzyme activity and the inhibition of the compound on the enzyme activity. The positive control compound was the phosphatase inhibitor sodium vanadate (Na 3 VO 4 ). Wherein the control group is the reagent control group. The results are shown in Table 2.

[0031] The formula for determining the relative inhibition rate of gene recombinant PTP1B at different concentrations is as follows:

[0032]

[0033]

[0034] The results show that: black raspberry fruit and leaf extracts (BRBFE, BRBLE) have a significa...

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Abstract

The invention relates to an application of a black raspberry extract in preparing an anti-diabetic medicine, and an application of the black raspberry extract in preventing type-II diabetes mainly characterized by glucose metabolic disorders, beta islet cell damage and insulin resistance. A novel anti-diabetic medicine preparation provided by the invention takes the black raspberry extract as an active ingredient, and the total effective dose is 1-100mg / kg / d. According to the novel anti-diabetic medicine preparation provided by the invention, the black raspberry extract can be compounded with the diabetes treatment medicines on the current market including sulfonylureas, biguanides or thiazolidinediones to prepare a compound preparation. The black raspberry extract can promote islet cell multiplication and inhibit the PTP1B activity; and meanwhile, in-vivo study finds that the black raspberry extract can be used for improving the increase of the mice blood glucose level caused by alloxan and has an obvious effect in reducing blood glucose. Through verification, the blood glucose reducing effect of the black raspberry fruit or leaf extract is superior to that of the selected positive medicine diaformin.

Description

technical field [0001] The invention belongs to the technical field of medicine, and mainly relates to the application of black raspberry extract (including black raspberry ripe fruit and black raspberry leaf extract) in the preparation of medicines for treating diabetes. Background technique [0002] Diabetes mellitus is a series of systemic metabolic disorders caused by relative or absolute deficiency of insulin due to dysfunction of the pancreas, or decreased sensitivity of target cells to insulin. According to the cause of the disease and the distribution in different populations, diabetes is often divided into type I diabetes and type II diabetes clinically. 346 million people worldwide have diabetes. If effective measures are not taken, as the course of the disease progresses, diabetes will have many complications, which may damage the heart, blood vessels, eyes, kidneys and nerves. Diabetes and its complications have brought huge burdens to individuals, families, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P3/10A61K131/00A61K127/00
Inventor 毕秀丽赵余庆夏希纯刘威
Owner 赵红石
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