Method for preparing different tones of indigo pigments based on blueberry leaves

A technology of black rice leaves and indigo pigment, which is applied in the field of natural plant pigment preparation, can solve the problems of limited color selection, poor water solubility of blue pigment, and imperfect preparation method, etc., and achieves good stability and nutrition, Good purification effect and good impurity removal effect

Active Publication Date: 2018-12-28
JIANGNAN UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

But at present, the utilization of the leaf pigment of the black rice tree is still in the level of optimizing its extraction method to increase its extraction rate. The preparation method is not perfect and the pigment produced is melanin. lead to oxidative blackening
[0004] At present, there are only seven kinds of blue pigments allowed to be used in our country, four of which are synthetic pigments. The range of blue tones covered is not large for people to choose colors, and most of these six kinds of blue pigments are not water-soluble, so they must be used under certain conditions. In order to enhance its water-soluble effect
There is no invention in China to prepare indigo pigments of different shades based on the leaf pigments of the black rice tree, and the enzymatic hydrolysis method of using glucosidase to increase the production rate of indigo pigments is also proposed in this patent for the first time

Method used

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  • Method for preparing different tones of indigo pigments based on blueberry leaves
  • Method for preparing different tones of indigo pigments based on blueberry leaves
  • Method for preparing different tones of indigo pigments based on blueberry leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Freeze-dry the leaves of Wufan tree, pass through a 60-mesh sieve after being crushed. Accurately weigh 10g of black rice leaf powder and add 200g of distilled water, carry out heat preservation water extraction (i.e. the ratio of solid to liquid is 1:20) under the condition of 30°C water bath, the extraction time is 2h, and the extraction times are 3 times to obtain the water extract.

[0043] The above-mentioned water extract was refrigerated and centrifuged for 10min (6000r / min) to obtain the supernatant, and the supernatant was filtered by an ultrafiltration membrane with a pore size of 30nm to obtain the crude extract of the leaves of the black rice. Pass the crude extract through AB-8 macroporous adsorption resin at a flow rate of 2BV / h, remove impurities with distilled water until the eluate is colorless, and then use 1BV of 10% ethanol to remove the adsorbed on the column at a flow rate of 1BV / h The non-pigment components were finally eluted with 6BV 60% ethanol...

Embodiment 2

[0053] Freeze-dry the leaves of Wufan tree, pass through an 80-mesh sieve after being crushed. Accurately weigh 10g of black rice leaf powder and add 400g of distilled water, and carry out heat preservation water extraction (i.e., the ratio of solid to liquid is 1:40) under the condition of 30°C water bath. The extraction time is 3h, and the extraction times are 4 times to obtain the water extract.

[0054] The above-mentioned water extract was refrigerated and centrifuged for 15min (6000r / min) to obtain the supernatant, and the supernatant was filtered through an ultrafiltration membrane with a pore size of 30nm to obtain the crude extract of the leaves of the black rice leaf. Pass the crude extract through AB-8 macroporous adsorption resin at a flow rate of 2BV / h, first remove impurities with distilled water until the eluate is colorless, and then use 2BV 15% ethanol to remove the adsorbed on the column at a flow rate of 2BV / h The non-pigment components were finally eluted w...

Embodiment 3

[0064] The operating conditions of embodiment 3 are shown in Table 1, and all the other operations and conditions are consistent with embodiment 2.

[0065] Concentrate the indigo aqueous solution obtained by evaporation under reduced pressure and then freeze-dry to obtain the finished indigo pigment. The color value is 92.1. The blue tone corresponds to the color number of the Pantone color card, and the color number close to it is PANTONG 2965C. The results are shown in Table 1 .

[0066] The above-mentioned indigo blue aqueous solution is heated to boiling, and its color has no obvious change.

[0067] Dissolving the above-mentioned finished indigo pigment in acidic solution with pH=3-4 or alkaline solution with pH=8-9, the color has no obvious change and can exist stably.

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Abstract

The invention discloses a method for preparing different tones of indigo pigments based on blueberry leaves. The method comprises the steps that blueberry leaves are used as raw materials, and different tones of indigo pigments are prepared by extracting and purifying pigments in the blueberry leaves and through reaction with multiple amino acids under the effect of glucosaccharase. The preparation method comprises the following steps of raw material drying and powdering, preparation of an enzyme buffer solution, water extraction pretreatment, filtration, blueberry leaf pigment crude extract purification, drying and concentration, enzymolysis for blue turning and finished product obtaining. By adopting the obtained natural indigo pigments, the product quality is stable, the safety is good,and the product has a better nutrition value. According to the types and use amounts of the added amino acids, different tones of indigo pigments can be prepared.

Description

technical field [0001] The invention belongs to the technical field of preparation of natural plant pigments, and in particular relates to a method for preparing indigo pigments of different hues based on the leaves of the black rice tree. Background technique [0002] Vaccinium bracteatum Thunb. is an evergreen plant of Rhododendronaceae Vaccinium. Its wild resources are widely distributed in my country, especially in Jiangsu, Zhejiang and Fujian. The nutritional value of black rice tree leaves and their fruits is very high. It is a custom in Jiangsu, Zhejiang and Fujian to make black rice with black rice leaves and fruits. Contain abundant polysaccharide and flavonoid compound in the leaf of black rice tree, also contain nutritional labelings such as multiple fatty acids, amino acid and vitamin simultaneously. Among its main components, carbohydrates account for 52.10%, and the content of flavonoids is about 19.48% (of which quercetin accounts for 35.71%). In recent year...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/16C09B7/02C09B61/00A23L5/47
CPCA23V2002/00A23L5/47C09B7/02C09B61/00C12P17/165A23V2200/044
Inventor 王立练伟佳樊铭聪钱海峰李言张晖齐希光吴港城
Owner JIANGNAN UNIV
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