Method for degrading corn straws

A technology of corn stalks and magnetic nanoparticles, applied in biochemical equipment and methods, fixed on or in inorganic carriers, fixed on/in organic carriers, etc. High production cost, deformation and inactivation, etc., to improve activity and stability, ensure cellulase activity, and increase affinity

Active Publication Date: 2020-02-28
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, laccase is not widely used in industry due to its high production cost, poor stability, and easy deformation and inactivation due to environmental changes during use.

Method used

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  • Method for degrading corn straws
  • Method for degrading corn straws
  • Method for degrading corn straws

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A method for degrading corn stalks, comprising the following steps:

[0046] (1) After the corn stalks are mechanically crushed, pass through a 40-mesh sieve, and then dry at 105° C. for later use;

[0047] (2) Cu 2+ Modified Fe 3 O4-NH 2 -PEI magnetic nanoparticles were dissolved in NaAC-HAC buffer solution containing laccase, and treated with shaking at 150 rpm for 40min at 25°C; after the reaction, the prepared Cu 2+ Modified Fe 3 O4-NH 2 - PEI laccase magnetic nanoparticles and supernatant separation; ultrapure water for Cu 2+ Modified Fe 3 O4-NH 2 -PEI laccase magnetic nanoparticles were washed continuously until no laccase activity was detected in the detergent; the pH of NaAC-HAC buffer was 4.6, the concentration of laccase was 0.5mg / mL, Cu 2+ Modified Fe 3 O4-NH 2 -The mass volume ratio of PEI magnetic nanoparticles to NaAC-HAC buffer solution is 1mg: 10mL;

[0048] (3) Shake the immobilized laccase prepared in step (2), the corn stalks treated in ste...

Embodiment 2

[0052] A method for degrading corn stalks, comprising the following steps:

[0053] (1) After the corn stalks are mechanically crushed, pass through a 40-mesh sieve, and then dry at 105° C. for later use;

[0054] (2) Immobilize laccase on Cu 2+ Modified Fe 3 O4-NH 2 -On PEI magnetic nanoparticles, immobilized laccase was formed, specifically Cu 2+ Modified Fe 3 O4-NH 2 -PEI magnetic nanoparticles were dissolved in NaAC-HAC buffer solution containing laccase, and shaken at a rate of 150 rpm for 70 min at 25°C; after the reaction, the prepared Cu 2+ Modified Fe 3 O4-NH 2 - PEI laccase magnetic nanoparticles and supernatant separation; ultrapure water for Cu 2+ Modified Fe 3 O4-NH 2 -PEI laccase magnetic nanoparticles were washed continuously until no laccase activity was detected in the detergent; the pH of NaAC-HAC buffer was 4.6, the concentration of laccase was 4mg / mL, Cu 2+ Modified Fe 3 O4-NH 2 -The mass volume ratio of PEI magnetic nanoparticles to NaAC-HAC b...

Embodiment 3

[0059] A method for degrading corn stalks, comprising the following steps:

[0060] (1) After the corn stalks are mechanically crushed, pass through a 40-mesh sieve, and then dry at 105° C. for later use;

[0061] (2) Immobilize laccase on Cu 2+ Modified Fe 3 O4-NH 2 -On PEI magnetic nanoparticles, immobilized laccase was formed, specifically Cu 2+ Modified Fe 3 O4-NH 2 -PEI magnetic nanoparticles were dissolved in NaAC-HAC buffer solution containing laccase, and shaken at a rate of 150 rpm for 50 min at 25°C; after the reaction, the prepared Cu 2+ Modified Fe 3 O4-NH 2 - PEI laccase magnetic nanoparticles and supernatant separation; ultrapure water for Cu 2+ Modified Fe 3 O4-NH 2 - PEI laccase magnetic nanoparticles were washed continuously until no laccase activity was detected in the detergent; the pH of NaAC-HAC buffer was 4.6, the concentration of laccase was 20.5 mg / mL, Cu 2+ Modified Fe 3 O4-NH 2 -The mass volume ratio of PEI magnetic nanoparticles to NaAC-...

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Abstract

A method for degrading corn straws is characterized by comprising the following steps: specifically mechanically crushing, sieving and drying the corn straws, then degrading lignin in the corn strawsby using immobilized laccase taking Cu2+ modified Fe3O4-NH2-PEI magnetic nanoparticles as carriers, recovering the immobilized laccase, boiling the degraded corn straws, then adding NaOH for washing,and adding cellulase for further degrading the cellulose. According to the method, the affinity between the immobilized laccase and a substrate is enhanced, the stability and the activity of the laccase in an application process are improved, thus, the degradation efficiency is improved, and the degradation rate of the lignin reaches 40.76% or above. Inhibition of the immobilized laccase and degradation products such as phenolic substances generated by degradation on the cellulase activity is eliminated, so that the cellulase activity is guaranteed, and the conversion rate of the cellulose isimproved, and reaches 46.85%.

Description

technical field [0001] The invention belongs to the field of straw degradation, and in particular relates to a method for degrading corn straw with immobilized laccase. Background technique [0002] Corn stover is a very abundant renewable resource, and the lignocellulose contained in it is the most valuable bioenergy. The lignocellulose composition and structure of different plants are different, and the lignocellulose of corn stover is mainly composed of cellulose, hemicellulose and lignin. However, cellulose, hemicellulose, and lignin in corn stover are intertwined to form a cell wall structure. This intertwined structure determines that the degradation of any type of component must be restricted by other components, especially lignin and hemicellulose. The cellulose is bound in the form of covalent bonds to wrap the cellulose, forming a natural barrier that hinders the contact between cellulase and cellulose molecules, thus limiting the decomposition of cellulose and ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12N11/14C12N11/082D21C5/00
CPCC12N9/0061C12N11/08C12N11/14C12P19/14C12P2201/00C12Y110/03002D21C5/005
Inventor 孙旸陈光苟泽昌孙春玉苏瑛杰雷志鹏张斯童吴卓夫王撼宇卢芳邓青青顾剑锋
Owner JILIN AGRICULTURAL UNIV
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