Pesticide residue degradation preparation based on agricultural plants and preparation method of pesticide residue degradation preparation
Through the compound preparation of laccase, lipase and catalase, the problem of difficult to efficiently remove pesticide residues in agricultural plants was solved, and the efficient degradation of multiple pesticide residues and environmentally friendly effects were achieved.
Patent Information
- Application Number
- CN202510881751.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-27
- Publication Date
- 2025-09-19
AI Technical Summary
Existing technologies make it difficult to efficiently and safely remove various pesticide residues from agricultural plants. Physical methods have limited effectiveness, chemical methods may introduce secondary pollution, and biological methods have long cycles and are greatly affected by the environment.
A compound preparation of laccase, lipase and catalase is used in combination with a stabilizer and a buffer to form an agricultural plant pesticide residue degradation preparation, which degrades various types of pesticide residues through synergistic effects.
It expands the degradation range of pesticide residues, improves degradation efficiency, extends the shelf life of preparations, is pollution-free to the environment, and meets the requirements of green agricultural development.
Smart Images
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Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of agricultural environmental protection, and in particular relates to a pesticide residue degradation preparation based on agricultural plants and a preparation method thereof. Background Art
[0002] Pesticides are widely used in agricultural production to control pests, diseases, and weeds, playing a vital role in ensuring crop yield and quality. However, this widespread use of pesticides has led to a serious problem of pesticide residues. These residues not only affect the quality and safety of agricultural products, threatening consumer health, but also pollute the soil, water, and other ecological environments, disrupting the ecological balance.
[0003] Currently, the main methods for removing pesticide residues from agricultural plants include physical methods (such as cleaning and heating), chemical methods (such as oxidation and reduction), and biological methods. Physical methods often have limited removal effects and are difficult to completely eliminate deep-seated pesticide residues. Chemical methods, while effective, may introduce new chemicals, causing secondary pollution. Furthermore, microbial degradation in biological methods suffers from long cycle times and significant environmental impact. Enzymes, as highly efficient and environmentally friendly biocatalysts, show great potential for pesticide residue degradation. Laccase, lipase, and catalase each have specific degradation capabilities for specific types of pesticides, but research and application of these three enzyme combinations for pesticide residue degradation in agricultural plants is limited. Therefore, the development of efficient, safe, and environmentally friendly pesticide residue degradation agents based on laccase, lipase, and catalase, as well as methods for their preparation, are of great practical significance. Summary of the Invention
[0004] The purpose of the present invention is to provide an agricultural plant pesticide residue degradation preparation based on laccase, lipase and catalase and a preparation method thereof. The preparation can efficiently degrade various types of pesticide residues on agricultural plants and has the advantages of high degradation efficiency, good safety and no pollution to the environment.
[0005] Technical Solution
[0006] A laccase, lipase, and catalase-based formulation for degrading agricultural plant pesticide residues comprises the following components, calculated by mass: 0.5-3% laccase, 0.3-2.5% lipase, 0.2-2% catalase, 1-5% stabilizer, 5-15% buffer, and the balance water. The stabilizer is a combination of one or more of sodium alginate, chitosan, and glycerol; the buffer is a sodium dihydrogen phosphate-sodium dihydrogen phosphate buffer, a citric acid-sodium citrate buffer, or a Tris-HCl buffer. The preparation method for the above-mentioned agricultural plant pesticide residue-degrading formulation comprises the following steps:
[0007] Laccase, lipase, catalase, stabilizer, buffer and water were weighed according to the formula ratio.
[0008] Add the buffer into water and stir evenly to prepare a buffer solution.
[0009] Add stabilizers to the buffer solution in sequence and stir until completely dissolved.
[0010] Laccase, lipase and catalase were slowly added to the above solution respectively, while stirring to allow the enzymes to be fully dissolved and dispersed.
[0011] Stir the mixed solution at 20-30°C for 30-60 minutes to ensure that all components are fully mixed.
[0012] The mixed solution is filtered and sterilized using a 0.22 μm filter membrane to obtain the agricultural plant pesticide residue degradation preparation.
[0013] Effects of the Invention
[0014] The degradation preparation of the present invention adopts a compound of laccase, lipase and catalase. The three enzymes can degrade various types of pesticide residues such as organophosphorus, organochlorine, pyrethroids, etc. through synergistic action, thereby expanding the scope of pesticide residue degradation and improving degradation efficiency.
[0015] The stabilizer used in the preparation can effectively protect the activity of the enzyme and extend the shelf life of the preparation; the buffer maintains the appropriate pH environment for the enzymatic reaction and ensures the catalytic activity of the enzyme.
[0016] The preparation method of the preparation is simple and easy to industrialize; and the preparation is a biological preparation, which will not cause pollution to the environment after use, has high safety, and meets the requirements of green agricultural development.
[0017] Production steps:
[0018] ( Figure 1 ) is the production flow chart
[0019] In the accompanying description ( Figure 1 ) is the production flow chart
[0020] The raw materials were weighed according to the formula ratio: laccase, lipase, catalase, stabilizer (one or more combinations of sodium alginate, chitosan, and glycerol), buffer (one of disodium hydrogen phosphate-sodium dihydrogen phosphate buffer, citric acid-sodium citrate buffer, and Tris-HCl buffer), and water.
[0021] Add the buffer into water and stir evenly to prepare a buffer solution;
[0022] Add stabilizers to the buffer solution in sequence and stir until completely dissolved;
[0023] Slowly add laccase, lipase and catalase to the above solution respectively, stirring while adding to allow the enzymes to fully dissolve and disperse;
[0024] Stir the mixed solution at 20-30°C for 30-60 minutes to ensure that all components are fully mixed;
[0025] The mixed solution was filtered and sterilized using a 0.22 μm filter membrane to obtain an agricultural plant pesticide residue degradation preparation;
[0026] A preparation is obtained which can be used to degrade pesticide residues in agricultural plants. DETAILED DESCRIPTION
[0027] Example 1
[0028] A laccase, lipase and catalase-based preparation for degrading agricultural plant pesticide residues comprises the following components, calculated by mass percentage: 0.5% laccase, 0.3% lipase, 0.2% catalase, 1% sodium alginate, 5% disodium hydrogen phosphate-sodium dihydrogen phosphate buffer, and the balance being water.
[0029] Preparation method:
[0030] Weigh each component according to the formula ratio:
[0031] Add disodium hydrogen phosphate-sodium dihydrogen phosphate buffer to water and stir evenly to prepare a buffer solution;
[0032] Add sodium alginate to the buffer solution and stir until completely dissolved;
[0033] Slowly add laccase, lipase and catalase to the above solution respectively, stirring while adding to allow the enzymes to fully dissolve and disperse;
[0034] The mixed solution was stirred at 20°C for 30 minutes to ensure that all components were fully mixed;
[0035] The mixed solution was filtered and sterilized using a 0.22 μm filter membrane to obtain an agricultural plant pesticide residue degradation preparation.
[0036] Example 2
[0037] A laccase, lipase and catalase-based preparation for degrading agricultural plant pesticide residues comprises the following components by mass percentage: 3% laccase, 2.5% lipase, 2% catalase, 3% chitosan, 10% citric acid-sodium citrate buffer, and the balance water.
[0038] Preparation method:
[0039] Weigh each component according to the formula ratio;
[0040] Add citric acid-sodium citrate buffer to water and stir evenly to prepare a buffer solution;
[0041] Add chitosan to the buffer solution and stir until completely dissolved;
[0042] Slowly add laccase, lipase and catalase to the above solution respectively, stirring while adding to allow the enzymes to fully dissolve and disperse;
[0043] The mixed solution was stirred at 25°C for 45 minutes to ensure that all components were fully mixed;
[0044] The mixed solution was filtered and sterilized using a 0.22 μm filter membrane to obtain an agricultural plant pesticide residue degradation preparation.
[0045] Example 3
[0046] A laccase, lipase and catalase-based preparation for degrading agricultural plant pesticide residues comprises the following components, calculated by mass percentage: 1.5% laccase, 1.2% lipase, 1% catalase, 2% glycerol, 15% Tris-HCl buffer, and the balance water.
[0047] Preparation method:
[0048] Weigh each component according to the formula ratio;
[0049] Add Tris-HCl buffer to water and stir evenly to prepare a buffer solution;
[0050] Add glycerol to the buffer solution and stir evenly;
[0051] Slowly add laccase, lipase and catalase to the above solution respectively, stirring while adding to allow the enzymes to fully dissolve and disperse;
[0052] The mixed solution was stirred at 30°C for 60 minutes to ensure that all components were fully mixed;
[0053] The mixed solution was filtered and sterilized using a 0.22 μm filter membrane to obtain an agricultural plant pesticide residue degradation preparation.
[0054] Effect verification
[0055] Vegetables contaminated with an organophosphorus pesticide (dichlorvos), an organochlorine pesticide (DDT), and a pyrethroid pesticide (cypermethrin) were treated with the degradation formulations prepared in the above examples. A water treatment was also used as a control. The treatment method involved diluting the degradation formulation 100-fold, soaking the vegetables in it for 30 minutes, then removing them and rinsing them with clean water. High-performance liquid chromatography and gas chromatography were used to determine pesticide residues in the vegetables before and after treatment, respectively. The results are shown in the following table:
[0056] Table 1 Implementation treatment comparison table: Table 1: Implementation treatment comparison table
[0057] As can be seen from the data in Table 1, the degradation preparation prepared by the present invention has a significant degradation effect on various pesticide residues, can effectively reduce the amount of pesticide residues on agricultural plants, and ensure the quality and safety of agricultural products.
Claims
1. A laccase, lipase and catalase-based formulation for degrading agricultural plant pesticide residues, characterized in that: The invention comprises the following components by mass percentage: laccase 0.5-3%, lipase 0.3-2.5%, catalase 0.2-2%, stabilizer 1-5%, buffer 5-15%, and the balance is water.
2. The agricultural plant pesticide residue degradation preparation according to claim 1, characterized in that The stabilizer is one or more combinations of sodium alginate, chitosan and glycerol.
3. The agricultural plant pesticide residue degradation preparation according to claim 1, characterized in that The buffer is one of disodium hydrogen phosphate-sodium dihydrogen phosphate buffer, citric acid-sodium citrate buffer, and Tris-HCl buffer.
4. The method for preparing the agricultural plant pesticide residue degradation preparation according to any one of claims 1 to 3, characterized in that: The following steps are involved: Laccase, lipase, catalase, stabilizer, buffer and water were weighed according to the formula ratio; Add the buffer into water and stir evenly to prepare a buffer solution; Add stabilizers to the buffer solution in sequence and stir until completely dissolved; Slowly add laccase, lipase and catalase to the above solution respectively, stirring while adding to allow the enzymes to fully dissolve and disperse; Stir the mixed solution at 20-30°C for 30-60 minutes to ensure that all components are fully mixed; The mixed solution is filtered and sterilized using a 0.22 μm filter membrane to obtain the agricultural plant pesticide residue degradation preparation.