Formula and preparation process of environment-friendly paint remover capable of being rapidly stripped at normal temperature

Abstract

The invention discloses a formula and a preparation process of an environment-friendly paint remover capable of being rapidly stripped at normal temperature, and relates to the technical field of paint removers, and an efficient viscosity reducer comprises the following components: a paint layer decomposing enzyme, a cellulose protecting enzyme, a buffer system, a solvent and a surfactant. According to the invention, high-activity fungal laccase modified by genetic engineering is adopted as a paint layer decomposing enzyme, the enzyme can decompose a paint molecular chain in a targeted manner at normal temperature, the paint removal efficiency is remarkably improved, meanwhile, cellulase capable of forming a protective film on the surface of a base material is cooperatively used, the base material is effectively protected from being damaged, and the combination not only realizes rapid paint removal, but also has the advantages of simple operation and low cost. The pollution to the environment is also reduced, and the green and environment-friendly production requirements are met.

Description

Technical Field

[0001] The invention relates to the technical field of paint removers, in particular to a formula and a preparation process of a quick-peeling, environment-friendly paint remover at room temperature. Background Art

[0002] With the rapid development of industry and construction, paint removal has become an important process. Whether in equipment maintenance, renovation or waste disposal, efficient and environmentally friendly paint removal is a key step.

[0003] Traditional paint removal methods have many shortcomings. First, although mechanical polishing is direct, it is inefficient and can easily scratch or deform the surface of the substrate, affecting subsequent use. Second, although chemical solvent immersion can dissolve paint quickly, the solvents used are mostly organic solvents, which are volatile and toxic, posing a serious threat to the health of operators and the environment. In addition, the waste generated by these methods is difficult to dispose of and can easily cause secondary pollution. Although there are some environmentally friendly paint removers on the market, they often need to be used at higher temperatures, which increases energy consumption and operational complexity, and the paint removal efficiency still needs to be improved.

[0004] In view of the problems of low efficiency, serious environmental pollution and damage to the substrate in traditional paint removal methods, the present invention proposes a formula and preparation process of a room temperature fast-peeling environmentally friendly paint remover, which is particularly important. Summary of the Invention

[0005] The purpose of the present invention is to make up for the shortcomings of the existing technology and provide a formula and preparation process of an environmentally friendly paint remover that can be quickly peeled off at room temperature. It can use highly active fungal lacquerase modified by genetic engineering as a paint layer decomposition enzyme, and can efficiently target and decompose paint molecular chains at room temperature. At the same time, it is used in conjunction with cellulase that can form a protective film on the surface of the substrate, effectively protecting the substrate from damage, improving the paint removal efficiency, and greatly reducing pollution to the environment.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions: a formula of a room temperature fast peeling environmentally friendly paint remover, which is prepared by the following raw materials in parts by weight: The lacquer layer decomposing enzyme, cellulose protecting enzyme, buffer system, solvent, surfactant; The paint layer decomposing enzyme is a lacquer enzyme that can target and decompose paint molecular chains; The cellulose protecting enzyme is a cellulase that can form a protective film on the surface of a substrate.

[0007] Furthermore, the laccase is a highly active fungal laccase that has been genetically modified, and its enzyme activity unit reaches 500-2000 U / mL when tested in a buffer system at a temperature of 25° C. and a pH value of 7.0 using catechol as a substrate.

[0008] Furthermore, the cellulase is derived from the fermentation product of a specific microbial strain that has been screened and domesticated. Its filter paper enzyme activity is 20-80U / mL, and it has strong tolerance to impurities such as lignin. In a simulated system containing 5% by mass lignin, the enzyme activity does not decrease by more than 20%.

[0009] Furthermore, the buffer system is composed of potassium dihydrogen phosphate and disodium hydrogen phosphate, the mass ratio of potassium dihydrogen phosphate to disodium hydrogen phosphate is between 1:3-3:1, and the pH value of the paint remover is adjusted to 6.5-7.5.

[0010] Furthermore, the solvent is deionized water and contains 3%-8% by mass of propylene glycol ether as a cosolvent. The amount of propylene glycol ether added is optimized and adjusted according to the hydrophilicity and hydrophobicity of the overall formula of the paint remover and the solubility characteristics of the enzyme to ensure that the paint remover does not stratify when left at 25°C for 24 hours.

[0011] Furthermore, the surfactant is a non-ionic surfactant Tween-80 with a mass fraction of 0.5%-2%. It has no inhibitory effect on enzyme activity and is well compatible with other ingredients in the paint remover, reducing the surface tension of the paint remover to 30-40mN / m.

[0012] On the other hand, a method for preparing a formula of a room temperature rapid peeling environmentally friendly paint remover is characterized in that the preparation method comprises the following steps: Pretreatment of lacquer layer decomposing enzyme: dissolve laccase powder in an appropriate amount of buffer solution containing specific additives, stir the reaction to fully dissolve and activate the laccase, and improve its catalytic activity; Activation of cellulose protective enzyme: Mix the original cellulase enzyme solution with a certain amount of activator and incubate it in a specific temperature environment to complete the activation process of cellulase and enhance its ability to form a protective film; Mixing preparation: Under stirring conditions, slowly add the pretreated lacquer layer decomposing enzyme solution to the mixed solution containing the buffer system, solvent and surfactant to evenly disperse the lacquer layer decomposing enzyme. Subsequently, add the activated cellulose protective enzyme solution dropwise to the above mixed solution and continue stirring to ensure that the two enzymes and various components are fully mixed to obtain a room temperature fast-peeling environmentally friendly paint remover.

[0013] Furthermore, during the pretreatment of the lacquer layer decomposing enzyme, laccase powder is dissolved in a buffer solution containing 0.05%-0.15% by mass of ethylenediaminetetraacetic acid, and stirred for 20-40 minutes at a temperature of 20-30°C and a rotation speed of 150-250 r / min.

[0014] Furthermore, during the activation of the cellulose protective enzyme, the cellulase original enzyme solution is mixed with a calcium chloride activator having a mass fraction of 0.02%-0.08%, and incubated in an environment with a temperature of 25-35°C for 40-80 minutes.

[0015] Compared with the existing technology, the formula and preparation process of this room temperature fast peeling environmentally friendly paint remover have the following beneficial effects: 1. The room-temperature fast-peeling and environmentally friendly paint remover of the present invention uses a highly active fungal lacquerase modified by genetic engineering as a paint layer decomposing enzyme. This enzyme can target and decompose paint molecular chains at room temperature, significantly improving the paint removal efficiency. At the same time, it is used in conjunction with cellulase that can form a protective film on the surface of the substrate to effectively protect the substrate from damage. This combination not only achieves rapid paint removal, but also reduces pollution to the environment, meeting green and environmentally friendly production requirements.

[0016] 2. The paint remover formula of the present invention ensures the stability of the paint remover at room temperature by optimizing the selection and ratio of the buffer system, solvent and surfactant. It does not stratify even when left at 25°C for 24 hours. In addition, the selected surfactant has no inhibitory effect on enzyme activity and is well compatible with other ingredients in the paint remover, further improving the overall performance and use effect of the paint remover.

[0017] Other advantages, objects and features of the present invention will be described in part in the following description and, in part, will be apparent to those skilled in the art based on an examination of the following or may be learned from the practice of the invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. Those skilled in the art can also derive other drawings based on these drawings without inventive effort.

[0019] Figure 1 This is a formula and preparation process flow chart of a room temperature fast-peeling environmentally friendly paint remover. DETAILED DESCRIPTION

[0020] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined purpose of the invention, the specific implementation methods, structures, features and effects of the present invention are described in detail below in conjunction with the accompanying drawings and preferred embodiments.

[0021] Examples 1 to 3 only change the content of the lacquer layer decomposing enzyme Example 1 Lacquer layer decomposing enzyme: a highly active fungal laccase expressed after genetic engineering was selected and tested in a buffer system at a temperature of 25°C and a pH value of 7.0 with catechol as a substrate. The enzyme activity unit was 1200 U / mL. 5 g of lacquerase powder was weighed. Cellulose-protecting enzyme: Select cellulase derived from the fermentation product of a specific microbial strain that has been screened and domesticated. The filter paper enzyme activity is 50 U / mL. In a simulated system containing 5% by mass lignin, the enzyme activity does not decrease by more than 20%. Prepare 200 mL of cellulase raw enzyme solution. Buffer system: Weigh 150g of potassium dihydrogen phosphate and 150g of disodium hydrogen phosphate in a mass ratio of 1:1 to adjust the pH value of the paint remover. Solvent: Prepare 1000 mL of deionized water and 60 g of propylene glycol ether (6% by mass of the solvent).

[0022] Surfactant: Measure the non-ionic surfactant Tween-80 to a mass fraction of 1%, i.e. 10 g. 5 g of laccase powder was dissolved in 250 mL of a buffer solution containing 0.1% by mass of ethylenediaminetetraacetic acid, and stirred at 25°C and 200 r / min for 30 min to complete the pretreatment of the lacquer layer with the enzyme. Mix 200 mL of cellulase enzyme solution with 1.2 g (mass fraction 0.06%) of calcium chloride activator and incubate at 30°C for 60 minutes to fully activate the cellulase.

[0023] Under stirring conditions, the pretreated lacquer layer decomposing enzyme solution is slowly added to the mixed solution containing the buffer system, solvent and surfactant, and the stirring is continued to uniformly disperse the lacquer layer decomposing enzyme. Subsequently, the activated cellulose protective enzyme solution is added dropwise to the above mixed solution, and the stirring is continued for 20 minutes to ensure that the two enzymes and various components are fully mixed to obtain the finished product of the room temperature fast-peeling environmentally friendly paint remover.

[0024] Effect: Testing showed that the paint remover prepared in this example had a pH of 7.0; the stripping time for common paint coatings at room temperature was 60-90 minutes; the damage rate to the cellulose structure on the surface of the wood substrate was 3%; the volatile organic compound (VOC) content was 40 g / L; and after use, the residual laccase activity was 75% of the initial activity, and the residual cellulase activity was 68% of the initial activity.

[0025] Example 2 Lacquer layer decomposing enzyme: a highly active fungal laccase expressed after genetic engineering was selected and tested in a buffer system at 25°C and pH 7.0 with catechol as substrate. The enzyme activity unit was 1200 U / mL. 15 g of lacquerase powder was weighed. Cellulose-protecting enzyme: Select cellulase derived from the fermentation product of a specific microbial strain that has been screened and domesticated. The filter paper enzyme activity is 50 U / mL. In a simulated system containing 5% by mass lignin, the enzyme activity does not decrease by more than 20%. Prepare 200 mL of cellulase raw enzyme solution. Buffer system: Weigh 150g of potassium dihydrogen phosphate and 150g of disodium hydrogen phosphate in a mass ratio of 1:1 to adjust the pH value of the paint remover. Solvent: Prepare 1000 mL of deionized water and 60 g of propylene glycol ether (6% by mass of the solvent).

[0026] Surfactant: Measure the non-ionic surfactant Tween-80 to a mass fraction of 1%, i.e. 10 g. 15 g of laccase powder was dissolved in 750 mL of a buffer solution containing 0.1% by mass of ethylenediaminetetraacetic acid, and stirred at 25°C and 200 r / min for 30 min to complete the pretreatment of the lacquer layer with the enzyme. Mix 200 mL of cellulase enzyme solution with 1.2 g (mass fraction 0.06%) of calcium chloride activator and incubate at 30°C for 60 minutes to fully activate the cellulase.

[0027] Under stirring conditions, the pretreated lacquer layer decomposing enzyme solution is slowly added to the mixed solution containing the buffer system, solvent and surfactant, and the stirring is continued to uniformly disperse the lacquer layer decomposing enzyme. Subsequently, the activated cellulose protective enzyme solution is added dropwise to the above mixed solution, and the stirring is continued for 20 minutes to ensure that the two enzymes and various components are fully mixed to obtain the finished product of the room temperature fast-peeling environmentally friendly paint remover.

[0028] Effect: Testing showed that the paint remover prepared in this example had a pH of 7.0; the stripping time for common paint coatings at room temperature was 15-30 minutes; the damage rate to the cellulose structure on the surface of the wood substrate was 7%; the volatile organic compound (VOC) content was 45 g / L; and after use, the residual laccase activity was 65% of the initial activity, and the residual cellulase activity was 62% of the initial activity.

[0029] Example 3 Lacquer layer decomposing enzyme: a highly active fungal laccase expressed after genetic engineering was selected and tested in a buffer system at 25°C and pH 7.0 with catechol as substrate. The enzyme activity unit was 1200 U / mL. 20 g of lacquerase powder was weighed. Cellulose-protecting enzyme: Select cellulase derived from the fermentation product of a specific microbial strain that has been screened and domesticated. The filter paper enzyme activity is 50 U / mL. In a simulated system containing 5% by mass lignin, the enzyme activity does not decrease by more than 20%. Prepare 200 mL of cellulase raw enzyme solution. Buffer system: Weigh 150g of potassium dihydrogen phosphate and 150g of disodium hydrogen phosphate in a mass ratio of 1:1 to adjust the pH value of the paint remover. Solvent: Prepare 1000 mL of deionized water and 60 g of propylene glycol ether (6% by mass of the solvent).

[0030] Surfactant: Measure the non-ionic surfactant Tween-80 to a mass fraction of 1%, i.e. 10 g. 20 g of laccase powder was dissolved in 1000 mL of a buffer solution containing 0.1% by mass of ethylenediaminetetraacetic acid, and stirred at 25°C and 200 r / min for 30 min to complete the pretreatment of the lacquer layer decomposing enzyme. Mix 200 mL of cellulase enzyme solution with 1.2 g (mass fraction 0.06%) of calcium chloride activator and incubate at 30°C for 60 minutes to fully activate the cellulase.

[0031] Under stirring conditions, the pretreated lacquer layer decomposing enzyme solution is slowly added to the mixed solution containing the buffer system, solvent and surfactant, and the stirring is continued to uniformly disperse the lacquer layer decomposing enzyme. Subsequently, the activated cellulose protective enzyme solution is added dropwise to the above mixed solution, and the stirring is continued for 20 minutes to ensure that the two enzymes and various components are fully mixed to obtain the finished product of the room temperature fast-peeling environmentally friendly paint remover.

[0032] Effects: The pH value of the paint remover prepared in this embodiment is 7.0; the peeling time of common paint coating at room temperature is 10-20 minutes; the damage rate of cellulose structure on the surface of wooden substrate is 10%; the volatile organic compound (VOC) content is 48 g / L; the residual activity of paint enzyme after use is 60% of the initial activity, and the residual activity of cellulase is 60% of the initial activity.

[0033] Examples four to six only change the content of cellulose protective enzyme Example four Paint layer decomposition enzyme: high-activity fungal paint enzyme expressed by genetic engineering is selected, and its enzyme activity unit is 1200 U / mL when detected in a buffer system with a temperature of 25°C and a pH value of 7.0 and with catechol as a substrate. 10 g of paint enzyme powder is weighed. Cellulose protective enzyme: cellulase derived from the fermentation product of a specific microbial strain selected and domesticated is selected, and its filter paper enzyme activity is 50 U / mL. In a simulated system containing 5% lignin by mass fraction, the enzyme activity decreases by no more than 20%. 100 mL of cellulase enzyme solution is prepared. Buffer system: 150 g of potassium dihydrogen phosphate and 150 g of sodium phosphate dibasic are weighed, and the mass ratio of the two is 1:1, which is used to adjust the pH value of the paint remover. Solvent: 1000 mL of deionized water and 60 g of propylene glycol ether (6% by mass fraction of the solvent) are prepared.

[0034] Surfactant: 10 g of non-ionic surfactant Tween-80 is measured, and the mass fraction is 1%. The 10 g of paint enzyme powder is dissolved in 500 mL of a buffer solution containing 0.1% ethylenediaminetetraacetic acid, and the pretreatment of the paint layer decomposition enzyme is completed under the conditions of a temperature of 25°C and a rotation speed of 200 r / min for 30 min of stirring reaction. The 100 mL of cellulase enzyme solution is mixed with 0.6 g (0.06% by mass fraction) of calcium chloride activator, and the cellulase is fully activated by incubating in an environment with a temperature of 30°C for 60 min.

[0035] Under stirring conditions, the pretreated paint layer decomposition enzyme solution is slowly added to the mixed solution containing the buffer system, the solvent, and the surfactant, and the paint layer decomposition enzyme is uniformly dispersed by continuous stirring. Subsequently, the activated cellulose protective enzyme solution is added dropwise into the above-mentioned mixed solution, and the stirring is continued for 20 min to ensure that the two enzymes and the components are fully mixed and uniform. The room-temperature fast stripping environmentally friendly paint remover product is obtained.

[0036] Effects: Testing showed that the paint remover prepared in this example had a pH of 7.0; the stripping time for common paint coatings at room temperature was 30-60 minutes; the damage rate to the cellulose structure on the surface of the wood substrate was 12%; the volatile organic compound (VOC) content was 42 g / L; and after use, the residual laccase activity was 70% of the initial activity, and the residual cellulase activity was 60% of the initial activity.

[0037] Example 5 Lacquer layer decomposing enzyme: a highly active fungal laccase expressed after genetic engineering was selected and tested in a buffer system at a temperature of 25°C and a pH value of 7.0 with catechol as a substrate. The enzyme activity unit was 1200 U / mL. 10 g of lacquerase powder was weighed. Cellulose-protecting enzyme: Select cellulase derived from the fermentation product of a specific microbial strain that has been screened and domesticated. The filter paper enzyme activity is 50 U / mL. In a simulated system containing 5% by mass lignin, the enzyme activity does not decrease by more than 20%. Prepare 300 mL of cellulase raw enzyme solution. Buffer system: Weigh 150g of potassium dihydrogen phosphate and 150g of disodium hydrogen phosphate in a mass ratio of 1:1 to adjust the pH value of the paint remover. Solvent: Prepare 1000 mL of deionized water and 60 g of propylene glycol ether (6% by mass of the solvent).

[0038] Surfactant: Measure the non-ionic surfactant Tween-80 to a mass fraction of 1%, i.e. 10 g. 10 g of laccase powder was dissolved in 500 mL of a buffer solution containing 0.1% by mass of ethylenediaminetetraacetic acid, and stirred at 25°C and 200 r / min for 30 min to complete the pretreatment of the lacquer layer decomposing enzyme. 300 mL of cellulase original enzyme solution was mixed with 1.8 g (mass fraction 0.06%) of calcium chloride activator and incubated at a temperature of 30°C for 60 minutes to fully activate the cellulase.

[0039] Under stirring conditions, the pretreated lacquer layer decomposing enzyme solution is slowly added to the mixed solution containing the buffer system, solvent and surfactant, and the stirring is continued to uniformly disperse the lacquer layer decomposing enzyme. Subsequently, the activated cellulose protective enzyme solution is added dropwise to the above mixed solution, and the stirring is continued for 20 minutes to ensure that the two enzymes and various components are fully mixed to obtain the finished product of the room temperature fast-peeling environmentally friendly paint remover.

[0040] Effects: The pH value of the paint remover prepared in this embodiment is 7.0; the peeling time of common paint coating at room temperature is 30-60 minutes; the damage rate of cellulose structure on the surface of wooden substrate is 2%; the volatile organic compound (VOC) content is 43 g / L; the residual activity of paint enzyme after use is 70% of the initial activity, and the residual activity of cellulase is 70% of the initial activity.

[0041] Example Six Paint layer decomposition enzyme: a high-activity fungal paint enzyme expressed by genetic engineering was selected, and its enzyme activity unit was 1200 U / mL when detected in a buffer system with a temperature of 25°C and a pH value of 7.0 and with pyrocatechol as a substrate. 10 g of paint enzyme powder was weighed. Cellulose protective enzyme: cellulase derived from the fermentation product of a specific microbial strain selected and domesticated, with a filter paper enzyme activity of 50 U / mL, and the enzyme activity decreased by no more than 20% in a simulated system containing 5% lignin by mass. 400 mL of cellulase enzyme stock solution was prepared. Buffer system: 150 g of potassium dihydrogen phosphate and 150 g of sodium phosphate dibasic were weighed, with a mass ratio of 1:1, for adjusting the pH value of the paint remover. Solvent: 1000 mL of deionized water and 60 g of propylene glycol ether (6% by mass of the solvent) were prepared.

[0042] Surfactant: 10 g of non-ionic surfactant Tween-80 was measured, with a mass fraction of 1%. The 10 g of paint enzyme powder was dissolved in 500 mL of a buffer solution containing 0.1% ethylenediaminetetraacetic acid, and the pretreatment of the paint layer decomposition enzyme was completed by stirring at a temperature of 25°C and a speed of 200 r / min for 30 min. 3. Activation of cellulose protective enzyme The 400 mL of cellulase enzyme stock solution was mixed with 2.4 g (0.06% by mass) of calcium chloride activator, and incubated at a temperature of 30°C for 60 min to fully activate the cellulase.

[0043] The pretreated paint layer decomposition enzyme solution was slowly added to the mixture containing the buffer system, solvent and surfactant under stirring, and the paint layer decomposition enzyme was uniformly dispersed by continuous stirring. Then, the activated cellulose protective enzyme solution was added dropwise into the above mixture, and the stirring was continued for 20 min to ensure that the two enzymes and the components were fully mixed and uniform. Finally, the room-temperature fast peeling environmentally friendly paint remover product was obtained.

[0044] Effect: The pH value of the paint remover prepared in this embodiment is 7.0; the peeling time of the paint remover on common paint coating at room temperature is 30-60 minutes; the damage rate of the cellulose structure on the surface of the wooden substrate is 1%; the volatile organic compound (VOC) content is 44 g / L; and the residual activity of the paint enzyme after use is 70% of the initial activity, and the residual activity of the cellulase is 72% of the initial activity.

[0045] The specific contents are shown in the following table: Change in pectinolytic enzyme content (g) Change in cellulolytic enzyme content (mL) Example One 5 200 Example Two 15 200 Example Three 20 200 Example Four 10 100 Example Five 10 300 Example Six 10 400 As shown in the above table, it can be seen from Examples 1 to 3 that, under the condition that other raw material parameters and preparation processes remain unchanged, the content of paint layer decomposition enzyme has a significant influence on the performance of the paint remover. When the content of paint layer decomposition enzyme decreases (Example 1), the peeling time of the paint remover on the paint coating is prolonged, but the protective effect on the wooden substrate is better, and the residual proportion of enzyme activity is also relatively high. With the increase of the content of paint layer decomposition enzyme (Example 2 and Example 3), the paint removal speed is significantly accelerated, but the damage rate of the cellulose structure on the wooden substrate increases, and the residual activity of the paint enzyme and cellulase after use shows a downward trend. At the same time, the pH value of the paint remover of the three examples is stable at 7.0, and the volatile organic compound (VOC) content is lower than the national standard of 50 g / L, maintaining good environmental performance. This shows that in actual production and application, the content of paint layer decomposition enzyme can be flexibly adjusted according to different needs for paint removal efficiency, substrate protection, and enzyme activity maintenance, so as to prepare a normal-temperature fast peeling environmentally friendly paint remover that is more suitable for the use scene.

[0046] It can be clearly found from the above Examples 4 to 6 that, under the condition that the content of paint layer decomposition enzyme and other raw material parameters and preparation processes remain unchanged, the content of cellulose protective enzyme has a significant influence on the performance of the paint remover. When the content of cellulose protective enzyme is low (Example 4), the protective ability of the paint remover on the cellulose structure on the surface of the wooden substrate is weak, and the damage rate reaches 12%, but the influence on the paint removal efficiency and the activity of the paint enzyme is small. With the increase of the content of cellulose protective enzyme (Example 5 and Example 6), the damage rate of the cellulose structure on the surface of the wooden substrate is greatly reduced to 2% and 1% respectively, and the protective effect on the substrate is significantly improved, and the residual activity of the cellulase itself is also improved. However, the change of the content of cellulose protective enzyme has little influence on the paint removal time and the volatile organic compound (VOC) content of the paint remover. The paint removal time of the three examples remains at 30-60 minutes, the VOC content is also lower than the national standard of 50 g / L, the pH value is stable at 7.0, and the residual activity of the paint enzyme is 70% of the initial activity. This shows that in actual production, the content of cellulose protective enzyme can be reasonably adjusted according to different needs for the degree of protection of the substrate, so as to realize effective protection of the wooden substrate under the premise of ensuring the paint removal efficiency and environmental performance.

[0047] The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as above in terms of a preferred embodiment, it is not intended to limit the present invention. Any person skilled in the art can, without departing from the scope of the technical solution of the present invention, make some changes or modifications to equivalent embodiments using the technical contents disclosed above. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A formula for a quick-peeling, environmentally friendly paint remover at room temperature, characterized in that: Made from the following raw materials by weight: The lacquer layer decomposing enzyme, cellulose protecting enzyme, buffer system, solvent, surfactant; The paint layer decomposing enzyme is a lacquer enzyme that can target and decompose paint molecular chains; The cellulose protecting enzyme is a cellulase that can form a protective film on the surface of a substrate.

2. The formula of a room temperature fast peeling environmentally friendly paint remover according to claim 1, characterized in that: The laccase is a highly active fungal laccase that has been genetically modified. The enzyme activity unit reaches 500-2000 U / mL when tested in a buffer system at a temperature of 25° C. and a pH value of 7.0 with catechol as a substrate.

3. The formula of a room temperature fast peeling environmentally friendly paint remover according to claim 1, characterized in that: The cellulase is derived from the fermentation product of a specific microbial strain that has been screened and domesticated. Its filter paper enzyme activity is 20-80 U / mL, and it has strong tolerance to lignin impurities. In a simulation system containing 5% by mass lignin, the enzyme activity does not decrease by more than 20%.

4. The formula of a room temperature fast peeling environmentally friendly paint remover according to claim 1, characterized in that: The buffer system consists of potassium dihydrogen phosphate and disodium hydrogen phosphate, the mass ratio of potassium dihydrogen phosphate to disodium hydrogen phosphate is between 1:3 and 3:1, and the pH value of the paint remover is adjusted to 6.5-7.

5.

5. The formula of a room temperature fast peeling environmentally friendly paint remover according to claim 1, characterized in that: The solvent is deionized water and contains 3%-8% by mass of propylene glycol ether as a cosolvent. The amount of propylene glycol ether added is optimized and adjusted according to the hydrophilicity and hydrophobicity of the overall paint remover formula and the solubility characteristics of the enzyme.

6. The formula of a room temperature fast peeling environmentally friendly paint remover according to claim 1, characterized in that: The surfactant is a non-ionic surfactant Tween-80 with a mass fraction of 0.5%-2%. The surfactant has no inhibitory effect on enzyme activity and is well compatible with other ingredients in the paint remover.

7. A method for preparing a room temperature fast peeling environmentally friendly paint remover, for preparing the formula of a room temperature fast peeling environmentally friendly paint remover according to any one of claims 1 to 6, characterized in that: The following steps are involved: Pretreatment of lacquer layer decomposing enzyme: dissolve laccase powder in an appropriate amount of buffer solution containing specific additives and stir to react; Activation of cellulose protective enzyme: Mix the original cellulase enzyme solution with a certain amount of activator and incubate it in a specific temperature environment to complete the activation process of cellulase; Mixing preparation: Under stirring conditions, slowly add the pretreated lacquer layer decomposing enzyme solution to the mixed solution containing the buffer system, solvent and surfactant to evenly disperse the lacquer layer decomposing enzyme. Subsequently, add the activated cellulose protective enzyme solution dropwise to the above mixed solution and continue stirring to ensure that the two enzymes and various components are fully mixed to obtain a room temperature fast-peeling environmentally friendly paint remover.

8. The formula of the room temperature fast peeling environmentally friendly paint remover according to claim 1, characterized in that: During the pretreatment of the lacquer layer decomposing enzyme, laccase powder is dissolved in a buffer solution containing 0.05%-0.15% by mass of ethylenediaminetetraacetic acid, and stirred for reaction for 20-40 minutes at a temperature of 20-30° C. and a rotation speed of 150-250 r / min.

9. The formula of the room temperature fast peeling environmentally friendly paint remover according to claim 1, characterized in that: During the activation of the cellulose protective enzyme, the cellulase original enzyme solution is mixed with a calcium chloride activator having a mass fraction of 0.02%-0.08%, and incubated in an environment with a temperature of 25-35° C. for 40-80 minutes.

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