Method of cultivating drought tolerance high fescue

A technology for tall fescue and seeds, which is applied in the fields of botanical equipment and methods, horticulture, and application to achieve the effects of high controllability, reliable technical measures, and easy and fast operation.

Inactive Publication Date: 2006-09-13
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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AI Technical Summary

Problems solved by technology

However, there are few reports on using the hypocotyls of tall fescue seedlings as explants to induce callus

Method used

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  • Method of cultivating drought tolerance high fescue
  • Method of cultivating drought tolerance high fescue
  • Method of cultivating drought tolerance high fescue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1, acquisition of drought-tolerant tall fescue and detection of transgenic plants thereof

[0056] 1. Induction of embryogenic callus

[0057] 1. Disinfection of seeds: Soak the seeds of tall fescue species antelope in sterile water for 1-3 hours, disinfect with 70% alcohol for 30 seconds, rinse with sterile water for 3-5 times, and disinfect with 50% sodium hypochlorite for 20-25 minutes, without stopping Shake vigorously and rinse with sterile water 3-5 times.

[0058] 2. Inoculate the sterilized tall fescue seeds on the seed inoculation medium, and cultivate them at 26 ± 1° C. under light 12h / d. After 3 days, the seeds begin to germinate, and about 20 days into seedlings ( figure 1 ).

[0059] 3. When the seedling grows to a height of about 2-5cm, under sterile conditions, cut about 0.5-1cm of the hypocotyl at the base of the seedling as an explant, such as figure 2 As shown, it was inoculated on the callus induction medium and cultured in the dark at ...

Embodiment 2

[0100] Example 2, analysis of glufosinate resistance of transgenic tall fescue plants

[0101] Select leaves with similar growth, area, and color from transgenic plants and control plants with basically similar morphological indicators such as plant height and growth, apply 2 mg / L glufosinate solution evenly on the leaves with a brush, and observe the growth of the leaves at any time. Variety. After smearing tall fescue leaves with glufosinate for 3 days, the leaves of the control plants began to show injury reactions, mainly manifested as leaf growth slowing down, the color of the leaves changed from dark green to light green, and the leaves of the transgenic plants turned from light yellow to white after 7 days, while the leaves of the transgenic plants Only slight injury symptoms appeared in individual parts. It was preliminarily confirmed that the Bar gene had been expressed in the recipient plants, endowing the recipient plants with glufosinate resistance, and the result...

Embodiment 3

[0102] Embodiment 3, the proline content determination of transgenic tall fescue plant

[0103] The proline content of tall fescue leaves of transgenic tall fescue plants with normal watering and control non-transgenic plants were determined. The determination method can refer to (Zhang Dianzhong, Wang Peihong, Zhao Huixian. Method for measuring free proline content of wheat leaves. Plant Physiol Letters, 1990, (4): 62-65). Take the average data of 4 independent experiments as the examination questions. Using the sulfosalicylic acid method, the proline content of the leaves of the normally watered transgenic plants and the control plants was measured, and the results were as follows: Figure 11 Shown (2-5 is the transgenic plant, 1 is the control plant). Tall fescue showed that the proline content of the control plants was 100%, and the proline content of the leaves of the transgenic plants was 31%-83% higher than that of the control plants, implying that the target gene had...

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Abstract

A method for culturing the drought-resistant Gaoyang cogongrass by genetic engineering features that the plant expression carrier is used to introduce the mutational P5CS-F129A gene of the delta1-pynoline-5-carboxylate synthetase of the cowpea to the embryonal callus, and after culture, the target drought-resistant Gaoyang cogongrass is obtained.

Description

technical field [0001] The invention relates to a method for cultivating new varieties of plants, in particular to a method for cultivating drought-tolerant tall fescue. Background technique [0002] Tall fescue (Festuca arundinacea Schreb.), also known as reed-like fescue, has extremely high nutritional value and is an important grass forage in the world. Because of its characteristics of drought resistance, barren resistance, disease resistance and wide adaptability, it is widely used in urban greening and sports field construction, and shows significant social, ecological and environmental benefits. Tall fescue is becoming more and more popular as a lawn grass. more and more people's attention. However, water resources in northern my country are relatively scarce, and tall fescue is used as a turfgrass, and its planting area is constantly expanding, and a large amount of water is needed for management. Therefore, it is urgent to cultivate new varieties of tall fescue tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00
Inventor 黄丛林吴忠义张秀海曹鸣庆李春华
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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