Agrobacterium mediated process of obtaining transgenic Testuca arundinacea

Abstract

The present invention provides agrobatcerium mediated process of obtaining transgenic Testuca arundinacea. The process includes dark culturing the callus of Testuca arundinacea in culture medium containing 2, 4-D in 2-5 mg / L for 2-3 to obtain embryonic callus, co-culturing the obtained embryonic callus together with agrobacterium carrying exogenous gene plasmid carrier to transfer the exogenous gene on the plasmid carrier into the embryonic callus genome of Testuca arundinacea, resistant callus screening and callus regenerating to obtain transgenic Testuca arundinacea plant. The improved agrobacterium mediated process has optimized induction of Testuca arundinacea callus, improved agrobacterium culturing method and raised agrobacterium transferring capacity in obtain transgenic Testuca arundinacea plant. The said process is effective, stable and simple, and has great application forground.

Description

(1) Technical field [0001] The invention relates to a method for obtaining transgenic tall fescue plants mediated by Agrobacterium, which belongs to the basic technology in the field of agricultural biotechnology and is used for improving the quality traits of lawn grass plants such as tall fescue. Through the Agrobacterium-mediated method, the exogenous target gene was introduced into the callus of tall fescue, and after callus differentiation, tall fescue with new traits was obtained. (2) Background technology [0002] Turfgrass planted in parks, golf courses and other places is multifunctional in controlling soil weathering, beautifying the environment, and providing sports and leisure places. With the increasing status of lawn industry in urban landscaping construction, it is developing into an industry with great potential, showing obvious social, ecological and environmental benefits in the market economy. Turfgrass seeds are the second most traded seeds in the world ...

AI Technical Summary

Problems solved by technology

Protoplasts are generally isolated from embryogenic suspensions, transformed by means of electric shock and PEG methods, and then cultivated and subcultured to regenerate plants; , 1995; Maas, 1994), purple fescue and tall fescue (Spangenberg, 1994) and other grasses are transformed by gene bombardment; however, the separation operation of plant protoplasts is complicated and the success rate is extremely low; and the imported fragment is determined The disadvantages such as weak sex, easy co-suppression and silencing of exogenous gene expression have become the restrictive factors of tall fescue genetic improvement.

[0006] The Agrobacterium-mediated method is currently the first method for the genetic transformation of dicotyledonous plants. Since Agrobacterium is not the natural host of monocotyledonous plants, it is rarely used for the genetic transformation of monocot tall fescue. At present, there are few Agrobacterium species of tall fescue. Related reports on mediated transformation method

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