Bipolaris yamadae (Y.Nisik.) shoemaker and screening and identification method and application thereof

A technology of umbilical umbilical bacteria and strains, applied in botany equipment and methods, biochemical equipment and methods, and microbial-based methods, etc., can solve the problem of no reports of biological control of Athenia mellifera and its related species, and no mountain fields. Helminthrosporium and other problems, to achieve the effect of low cost, crop safety, inhibition of drug resistance and development of drug resistance

Active Publication Date: 2020-08-11
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, domestically, there are patents on the use of Helminthosporium genus Setaria, Helminoptera maize, and Helminoptera jipingus as biological herbicides, but there is ...

Method used

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  • Bipolaris yamadae (Y.Nisik.) shoemaker and screening and identification method and application thereof
  • Bipolaris yamadae (Y.Nisik.) shoemaker and screening and identification method and application thereof
  • Bipolaris yamadae (Y.Nisik.) shoemaker and screening and identification method and application thereof

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Experimental program
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Embodiment 1

[0039] The new bacterial strain of the present invention is obtained through the following approach. The inventors have studied the strains in Anhui, Fujian, Guangdong, Guangxi, Guizhou, Henan, Hubei, Hunan, Jiangsu, Jiangxi, Sichuan, Shandong, Taiwan, Yunnan, Tibet and Zhejiang provinces. Investigate and collect the diseased leaves of Athenia mellifera in forest edge, grassland, wilderness, wetland, edge of crop field, ditches, hedges, canyons and farmland, and record the symptoms and relevant information of the plants and bring them back The laboratory conducted isolation research on its parasitic fungi and carried out pathogenicity verification in strict accordance with Koch's postulate.

[0040] First, the pathogenic symptoms of spores produced by Bipolaris yamadae (Y.Nisik.) Shoemaker strain (Bipolaris yamadae (Y.Nisik.) Shoemaker) HXDC-1-2 under natural conditions on A. Lesions of different sizes can be seen. In the early stage of onset, dark brown or black lesions appea...

Embodiment 2

[0049] Helminthrospora yamada HXDC-1-2 of the present invention is used for biological weeding, and the specific application method is: the bacterial classification is inoculated on the following culture medium, after inducing conidia, it is sprayed on the field or the place where weeds occur, Use a dose of 10 2 ~10 6 individual / mL.

[0050] The cultivation method is as follows: grow Helminthrospora Yamada under dark conditions for about 4 days, place the petri dish under aseptic conditions under black light or ultraviolet light (24h) and irradiate, and the culture medium will produce spores during the irradiation process .

[0051] PDA medium - potato dextrose agar medium

[0052]

[0053] At present, there is no report on the fungal herbicides of weeds on weeds at home and abroad. The research in this area provides a new and excellent way for the control of weeds in weeds and grasses. The herbicidal composition of the invention can be used in conjunction with other su...

Embodiment 3

[0056] The leaves of the diseased Azalea spp. were collected from the wild, the symptoms of the plants were recorded, and the microscopic examination was carried out. Isolation of pathogenic fungi was carried out on the agar medium of A. Pick single spores of each strain under a microscope, inoculate them on PDA medium, and cultivate them in the dark at 28°C. Observe the colony characteristics of pure culture of pathogenic fungi and measure the diameter of the colony. In addition, each colony disc with a diameter of 5 mm was inoculated in the center of the PDA plate, and the diameter of each colony was measured after 4 days.

[0057] Table 1 Comparison of the colony characteristics of the pathogenic fungi of Weeda mellifera

[0058]

[0059] Note: D1 represents the colony diameter formed after 5 days of single spores,

[0060] D2 represents the colony diameter formed after 4 days of 5mm colony disk.

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Abstract

The invention discloses a bipolaris yamadae (Y.Nisik.) shoemaker and a screening and identification method and application thereof. The bipolaris yamadae (Y.Nisik.) shoemaker (HXDC-1-2) has been deposited in the China General Microbiological Culture Collection Center with the accession number CGMCC No.17778. The bipolaris yamadae (Y.Nisik.) shoemaker is applied to biological weeding when the conidia concentration is 102 conidia/ml or more, can be safely applied to crop fields of rice, corn, soybeans, peanuts, sesame, millet, broad beans, peas, rape, vegetables, flowers, traditional Chinese medicinal materials and fruit trees, and is used for controlling gramineous weeds, such as barnyard grass, semen euphorbiae, herba digitariae, herba setariae viridis, eleusine indica, leptochloa panicea,wild oat, Aleppo grass and microstegium vimineum, annular nutgrass flatsedge and part of broad-leaf weeds; and the control effect can reach 80% or more. The bipolaris yamadae (Y.Nisik.) shoemaker, serving as a biological herbicide, has the advantages of being good in weeding effect, low in usage amount, high in safety to crops, low in cost, environment-friendly, free of a residue and free of pollution.

Description

technical field [0001] The invention belongs to the technical field of microorganisms applied to agricultural plant protection and control of weeds in agricultural crops, and specifically relates to a strain of Helminthrospora yamadae strain Bipolaris yamadae (Y.Nisik.) Shoemaker (HXDC-1-2), its screening and identification method and its use. Background technique [0002] Weed damage is one of the most important factors in reducing crop yields, costing global agriculture $95 billion annually. The area of ​​weed damage in China's farmland is about 78.8 million hectares. Even though 23.5 billion yuan is spent on weed control every year, it still causes 14.6 million tons of grain, cotton, and oil losses, and the direct economic loss is nearly 100 billion yuan. Barnyardgrass, wild oats, kanmai, crabgrass, daughter, goosegrass, foxtail, quinoa, and bermudagrass are the top ten harmful weeds in farmland, of which grasses account for 80%. Since humans began to engage in agricult...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12Q1/6895C12N15/11A01N63/30A01P13/00C12R1/645
CPCC12Q1/6895A01N63/30C12R2001/645C12N1/145A01P13/02A01N25/04
Inventor 强胜谭敏
Owner NANJING AGRICULTURAL UNIVERSITY
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