49results about How to "The enrichment effect is obvious" patented technology

Provided is a heavy metal pollution soil repairing agent using excess activated sludge as the rawmaterial, a method of extraction and a method for repairing the heavy metal pollution soil, which relates to a heavy metal pollution soil repairing agent and a method thereof and a method for repairing the heavy metal pollution soil, and solves the problems of high cost, easily secondary pollution and complex operation existing in the repair of the heavy metal pollution soil. The heavy metal pollution soil repairing agent is composed of amylose, protein, nucleic acid, phosphoric acid, amino acid, humic acid compound, uronic acid and the organic substance required for cell lifecycle. The method for distilling the heavy metal pollution soil repairing agent is as follows: the wastewater treatment excess activated sludge reacts under the condition of 80-120 KPa and 60-100 DEG for 8-20 minutes; then centrifuges for 8-20 min at the rotating speed of 5000-7000r/min. The invention adopts the home position repairing or the heterotopia repairing to repair the heavy metal pollution. The invention has low cost and no secondary pollution, simple operation, short repairing period, strong activity and wide range of application.

The invention discloses an electrochemical sensor based on a chiral covalent organic framework compound and belongs to the technical field of electrochemical detection. The electrochemical sensor disclosed by the invention is obtained by modifying the covalent organic framework compound COF-CTpPa-2 on a matrix electrode, and can be used for enriching and separating a small molecular electric active substance with a pi conjugated structure; when the molecular diameter of a substance is less than the pore diameter of the COF-CTpPa-2, the enrichment effect of the sensor on the substance is more obvious when the quantity of benzene rings of the substance is more; meanwhile, the electrochemical sensor also can be used for distinguishing and identifying the chiral compound.

The invention relates to a synergist for lactococcus lactis subsp. lactis capable of generating nisin through fermentation and a use method of the synergist. The synergist is prepared from, by weight, 0.02-1.5 parts of taurine, 0.003-0.006 part of selenium, 0.03-0.8 part of proline, 0.05-1.0 part of lysine and 0.02-0.052 part of inositol. The synergist is added into an M17 culture medium, and then lactic acid bacteria capable of generating the nisin are inoculated for fermentation. All the ingredients of the synergist are easy to obtain, low in purchase cost and simple in use method. Experiments show that the synergist can obviously improve the nisin yield of the lactic acid bacteria, synergia is obvious, therefore, the production cost of the nisin is reduced, the production cycle is shortened, and the production efficiency is improved.

The invention provides a polymer drug carrier and a method for preparing the same. The polymer drug carrier is provided with a core-shell structure, a core comprises polyethylene glycol-distearoyl phosphoethanolamine nano-micelle, and a shell comprises poly-dopamine. The method includes forming the nano-micelle from polyethylene glycol-distearoyl phosphoethanolamine; forming the poly-dopamine shell on the outside of the nano-micelle to obtain the polymer drug carrier. The polymer drug carrier and the method have the advantages that the core and the shell of the polymer drug carrier comprise biodegradable polymers, accordingly, the polymer drug carrier is good in biocompatibility, and few toxic and side effects can be realized; the particle size of the polymer drug carrier is smaller than 50nm, tumor gathering effects and photo-thermal therapy effects can be realized, and tumor cell killing effects can be realized after near-infrared light irradiates on the polymer drug carrier; drugs can be encapsulated by the nano-micelle of the polymer drug carrier, diversified drugs can be loaded under chemical modification effects of the poly-dopamine, and accordingly the polymer drug carrier has an excellent application prospect in the field of medicine and pharmacology.

The invention relates to a method for strengthening in-situ remediation of polluted water bodies in riverways and lakes by transgenic plants. The method is characterized by comprising the steps that 1, in view of the characteristics of difficulty in the growth of conventional plants in moderately and slightly polluted water bodies, eutrophic elements and heavy metal elements in the water bodies are transferred and fixed into bottom sediment by the transgenic plants with high adsorption efficiency and high resistance; and 2, through micro-topography transformation combing with plant configuration, polluted river sections and lake landform landscape are divided, erosion zones and sedimentary zones are artificially constructed, the initial conditions of transgenic plant growth are guaranteed,and the repair efficiency of the polluted river water bodies is improved. The method has the advantages such as high adaptability and environmental friendliness in strengthening the in-situ remediation of the polluted water bodies in the riverways and lakes, and has wide application prospects.

The invention relates to the field of catalysts, preparation methods and applications thereof, in particular to a MnY catalyst for catalytically removing indoor formaldehyde and a preparation method thereof. The catalyst is composed of a catalyst carrier NaY molecular sieve and an active component Mn. Manganeseacetylacetonate (II) is a manganese source of the active component Mn. The preparation method comprises the following steps: soaking the NaY molecular sieve in an acetone solution of manganeseacetylacetonate (II), and stirring the mixture till an acetone solvent is fully volatilized to obtain a catalyst precursor; and carrying out temperature programming activation in an activated atmosphere, and then cooling the mixture to room temperature and taking the same out to obtain the MnY catalyst. The MnY catalyst prepared by the method removes formaldehyde in indoor air. As Mn oxide active centers which have good catalytic effects in oxidizing and removing formaldehyde are dispersed to the surface of the Y molecular sieve, formaldehyde adsorbed to the surface of the Y molecular sieve is degraded to CO2 and H2O without being affected by adsorption capacity. The method is concise, quick and environment-friendly, and shows a relatively good catalytic performance in catalytic removal of indoor formaldehyde.

The invention relates to the technical field of mutation breeding of lactic acid bacteria, and provides a method for breeding weak post-acidification streptococcus thermophilus strains. The method comprises the following steps: S1, preparation of a bacterial suspension: culturing an original strain to obtain a bacterial solution, transferring and continuously culturing the bacterial solution to a logarithmic metaphase, centrifuging, washing and concentrating the bacterial solution to obtain the suspension, and placing the suspension in an empty plate for later use; S2, ultraviolet irradiation: placing the suspension under an ultraviolet lamp for irradiation; S3, rejuvenation culture: resuspending the bacteria obtained in S2 in a fresh culture medium for rejuvenation culture; S4, penicillin treatment: adding penicillin for treatment, performing dilution, and coating an MC culture medium with the solution for culture; and S5, screening weak post-acidification strains. According to the technical scheme, the problem that the weak post-acidification streptococcus thermophilus strain cannot be efficiently bred in the prior art is solved.

The invention relates to novel tag protein for protein enrichment expression and intracellular localization as well as application thereof. The tag protein is derived from membrane protein-reflectin A1(RA1) of a squid iridophore. The application of the novel tag protein for protein enrichment expression and intracellular localization comprises the following steps: (a) providing a construction object, wherein in an open reading frame of a gene expression vector, the construction object, from 5' to 3' segments, sequentially comprises the following operable connecting components: an encoding geneof eGFP and an encoding gene of the RA1; (b) transferring the construction object in (a) into a host cell so as to express eGFP and RA1 tags; (c) providing a group of recombinant protein formed by the eGFP and RA1 tags, wherein the recombinant protein contains functional areas of the eGFP and the RA1, and the eGFP exerts under the action of the RA1 functional area; and (d) performing intracellular enrichment and spatial orientation to be favorable for the subsequent separation and purification.

The invention discloses a metallothionein gene MT20. The base sequence of the metallothionein gene is shown as SEQ ID NO:1, the metallothionein gene is derived from soil microorganisms, is high in heavy metal tolerance and can still survive and grow in a cadmium solution with the concentration of 1.0 M. Moreover, the metallothionein gene has an obvious cadmium metal enrichment effect, so the metallothionein gene has a great application potential in industries such as heavy metal pollution control.

The invention relates to the technical field of ecological restoration of cadmium polluted soil, and concretely relates to a method for restoring cadmium polluted soil by Thalia dealbata. Researches find that Thalia dealbata can normally grow in high concentration cadmium environment, the biomass of the Thalia dealbata does not obviously decrease, the heavy metal content of the aboveground part of the Thalia dealbata is higher than the heavy metal content of the root part of the Thalia dealbata, and Thalia dealbata can adsorb cadmium in soil. Thalia dealbata is planted in the heavy metal cadmium polluted soil, so when Thalia dealbata plants are mature, the aboveground parts of the plants (a certain height of the basal parts of the plants are reserved) are cut, harvested and removed from the polluted soil; and after the base parts or the underground parts of the plants have new buds and the new buds gradually grow to form new mature plants, the above processes are repeated in order to reduce the pollutant cadmium in soil.

The invention discloses manganese-loaded plant-based biochar and a preparation method and application thereof. The loading capacity of Mn in the plant-based biochar is 25-30mg/g. The preparation method comprises the following steps: cultivating hyperaccumulators, and irrigating the hyperaccumulators with a manganese ion-containing solution in the growth process of the hyperaccumulators; harvestingmature plants, cleaning and drying; and crushing the dried plants, sieving with a 60-mesh sieve, and calcining at 480-550 DEG C for 1-4 hours to obtain the manganese-loaded plant-based biochar. The plant-based biochar can be used for adsorbing and curing heavy metal ions, and can be used for treating the environment polluted by heavy metals. The high-manganese-loaded plant-based charcoal is obtained through plant cultivation and calcination, exogenous loading treatment is not needed, the preparation process of the charcoal is simpler and more convenient, the risk of secondary pollution is reduced, and the charcoal is suitable for large-scale application and popularization.

The invention provides a method for analyzing sweet components in smoke in saliva in the oral cavity of a smoker, which comprises the following steps: heating and balancing the saliva obtained after the smoker smokes a cigarette, inserting an extraction rod into the saliva for oscillating, heating and extracting, taking out the extraction rod for thermal desorption, and measuring by adopting gas chromatography-mass spectrometry. Therefore, the content of the sweet component in the smoke in the saliva is determined. According to the analysis method for the smoke sweet component in the oral saliva of the smoker, comprehensive analysis on the trace smoke sweet component in a saliva complex matrix can be achieved, no organic reagent needs to be used in the detection process, and the method is non-toxic, safe, environmentally friendly, low in detection cost and good in popularization and application.

The invention discloses a titanate nanotube SPME coating and a preparation method and application thereof. The coating comprises a titanate nanotube layer attached to solid-phase microextraction fiber. The preparation method comprises the following steps: taking pretreated solid-phase microextraction fiber, coating the surface of the solid-phase microextraction fiber with polyimide resin, adding titanate nanotubes to the polyimide resin, and aging to prepare the titanate nanotube SPME coating. The titanate nanotube SPME coating can be directly used for PAHs analysis in natural water bodies, has a wider linear range and a lower detection limit for a plurality of PAHs, and simultaneously has a good enrichment effect on the plurality of PAHs.

The invention provides a method for analyzing aroma components in tobacco, which comprises the following steps: continuously heating a tobacco sample to be detected, enriching the aroma components in the tobacco by adopting a closed loop gas stripping method, performing thermal desorption, and performing qualitative and quantitative analysis by adopting a gas chromatograph-mass spectrometer. According to the method, the problem that the adsorption effect of the adsorbent becomes poor in the enrichment process can be effectively avoided, the method also has a good adsorption effect on the to-be-detected tobacco sample with the high moisture content, and the test of the aroma components in the tobacco is more accurate and effective.

The invention discloses a metallothionein gene MT18. The metallothionein gene MT18 has a base sequence shown as SEQ ID NO: 1, and derives from soil microorganisms; and the metallothionein gene MT18 isgood in heavy metal tolerance, and can survive and grow even in a cadmium solution of which the cadmium concentration is 1.0M. Moreover, the metallothionein gene MT18 has obvious cadmium metal enrichment effects; and thus, the metallothionein gene MT18 has relatively great application potential in heavy metal pollution control and other industries.