49results about How to "The enrichment effect is obvious" patented technology

The invention relates to a super-hydrophilic micro-well sensing interface for enrichment and trace detection and a preparation method of the super-hydrophilic micro-well sensing interface. The method comprises the following steps: repeatedly translating a clean substrate above candle flame at constant speed, carrying out physical deposition on the surface of the substrate to obtain uniformly-distributed carbon nanolayers with a certain thickness, carrying out chemical vapor deposition on silicon dioxide by taking the substrate as a template, and thus obtaining a nano-composite structure with carbon particles being coated by the silicon dioxide; calcining at high temperature to remove carbon cores, and thus obtaining a uniform hollow nano silicon dioxide layer with a micron thickness; after treating the surface of nano silicon dioxide by a plasma, modifying a silanization reagent on the surface by adopting a monomolecular-film self-assembly method; covering a circular photomask plate, and carrying out ultraviolet light degradation on the silanization reagent in the uncovered area by adopting a photoetching technique to obtain the super-hydrophilic micro-well sensing interface for enrichment and trace detection. By utilizing the advantage of a controllable patterning designated limited range, a super-hydrophilic micro-well has good concentration and enrichment effects on micro droplets of an extremely dilute solution and can be used for real-time trace detection of target molecules.

The invention relates to the field of biology, in particular to a method for preparing and using a sandwich type electrochemical immunosensor for detecting prostate specific antigen. The present invention sequentially modifies polydopamine and boron nitride composite (Au@PDA@BCN) loaded with gold nanoparticles on the electrode surface with good conductivity and gold with excellent enrichment effect on signal molecule methylene blue (MB). Platinum nanoparticles modified manganese dioxide covalent organic framework composite (AuPt@MnO2@TFPB‑COF); through two materials to achieve the amplification effect of electrochemical signals, the sandwich type detection of prostate specificity using peptide and antibody was constructed The electrochemical immunosensor for sex antigen has achieved the purpose of accurate and quantitative detection of prostate specific antigen.

The invention relates to a pretreatment method for detecting boron impurities of slagging constituent, in particular to a pretreatment method for detecting boron impurities of titanium dioxide, which comprises the following steps: adding nitric acid and hydrofluoric acid into titanium dioxide so as to enable titanium dioxide to be dissolved, adding propylene glycol to be subjected to complexation with boron after diluting acidity through adding water, performing oscillation and standing, extracting boron complex by adopting normal hexane, then performing standing stratification, heating the normal hexane phase of the upper layer till full volatilization, adding nitric acid into the residue, then adding water for dilution, and finally performing detection after constant volume. The method has the advantages as follows: the gathering function of boron is obvious, few titanium ions exist in the solution to be detected, the influence of the matrix effect is greatly reduced, and the extraction yield is greater than 90%; the recovery of the boron can be improved to more than 95% from 80-82%, and the method detection limit of the boron is reduced to 0.5 ppm from 2 ppm; in addition, the pollution to the sampling system of an apparatus is avoided, and the method is simple and easy to operate, and improves the detection accuracy.

The invention provides a polymer drug carrier and a method for preparing the same. The polymer drug carrier is provided with a core-shell structure, a core comprises polyethylene glycol-distearoyl phosphoethanolamine nano-micelle, and a shell comprises poly-dopamine. The method includes forming the nano-micelle from polyethylene glycol-distearoyl phosphoethanolamine; forming the poly-dopamine shell on the outside of the nano-micelle to obtain the polymer drug carrier. The polymer drug carrier and the method have the advantages that the core and the shell of the polymer drug carrier comprise biodegradable polymers, accordingly, the polymer drug carrier is good in biocompatibility, and few toxic and side effects can be realized; the particle size of the polymer drug carrier is smaller than 50nm, tumor gathering effects and photo-thermal therapy effects can be realized, and tumor cell killing effects can be realized after near-infrared light irradiates on the polymer drug carrier; drugs can be encapsulated by the nano-micelle of the polymer drug carrier, diversified drugs can be loaded under chemical modification effects of the poly-dopamine, and accordingly the polymer drug carrier has an excellent application prospect in the field of medicine and pharmacology.

The invention discloses a glioma diagnostic marker Circ6: 22020339|22020542 and an application. According to the invention, it is first found that an exosome secreted by glioma cells has an obvious enrichment effect on Circ6: 22020339|22020542, and the expression level of Circ6: 22020339|22020542 in the serum exosome of a patient who suffers from glioma is obviously reduced (p<0.0001) than that ofa control group. ROC curve analysis shows that the marker has relatively high diagnostic value (AUC=0.986, p<0.0001, the susceptibility is 100%, and the specificity is 92.9%) on glioma. Therefore, through detection of the expression level of Circ6: 22020339|22020542 in the serum exosome of a patient who suffers from glioma, noninvasive diagnosis can be early fast made to the patient who suffers from glioma.