Preparation and application method of a sandwich-type electrochemical immunosensor for detecting prostate-specific antigen
A prostate-specific immunosensor technology, which is applied in the field of preparation of sandwich electrochemical immunosensors, can solve the problems of poor stability, low sensitivity, and high cost of prostate-specific antigen detection, and achieves low cost, high sensitivity, and superior stability. Effect
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[0049] A preparation method of a sandwich type electrochemical immunosensor for detecting prostate specific antigen: comprising the following steps:
[0050] (1) Preparation of gold nanoparticles (AuNPs): Add 500 μL of 23.46 mol / L HAuCl in 50 mL of deionized water 4 Solution, heated at 110°C for 5min, then quickly added 5mL of freshly prepared sodium citrate solution with a concentration of 14.55mol / L, boiled back at 110°C for 30min until the solution turned wine red, and cooled to room temperature to obtain gold The nanoparticle dispersion was stored at 4°C for future use.
[0051] (2) Preparation of boron nitride (BCN): Take 5g of 4-pyridylboronic acid and place it in a tube furnace, and under the protection of nitrogen flow, heat the tube furnace to 800°C for calcination for 3h to obtain black powder boron nitride .
[0052] (3) Preparation of Au@PDA@BCN dispersion: Weigh 1 mg of boron nitride and 2 mg of dopamine prepared in step (2) respectively, and disperse them in Tr...
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