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Preparation method and use method of sandwich type electrochemical immunosensor for detecting prostate specific antigen

A technology of prostate-specific and immunosensors, which is applied in the field of preparation of sandwich-type electrochemical immunosensors, can solve the problems of poor stability, low sensitivity, and high cost of prostate-specific antigen detection, and achieve low cost, high sensitivity, superior electrical conductivity and The effect of stability

Active Publication Date: 2020-05-26
YUNNAN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing and using a sandwich-type electrochemical immunosensor for detecting prostate-specific antigen, so as to solve the existing problems of high detection cost, low sensitivity, and poor stability for prostate-specific antigen

Method used

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  • Preparation method and use method of sandwich type electrochemical immunosensor for detecting prostate specific antigen
  • Preparation method and use method of sandwich type electrochemical immunosensor for detecting prostate specific antigen
  • Preparation method and use method of sandwich type electrochemical immunosensor for detecting prostate specific antigen

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Embodiment 1

[0049] A preparation method of a sandwich type electrochemical immunosensor for detecting prostate specific antigen: comprising the following steps:

[0050] (1) Preparation of gold nanoparticles (AuNPs): Add 500 μL of 23.46 mol / L HAuCl in 50 mL of deionized water 4 Solution, heated at 110°C for 5min, then quickly added 5mL of freshly prepared sodium citrate solution with a concentration of 14.55mol / L, boiled back at 110°C for 30min until the solution turned wine red, and cooled to room temperature to obtain gold The nanoparticle dispersion was stored at 4°C for future use.

[0051] (2) Preparation of boron nitride (BCN): Take 5g of 4-pyridylboronic acid and place it in a tube furnace, and under the protection of nitrogen flow, heat the tube furnace to 800°C for calcination for 3h to obtain black powder boron nitride .

[0052] (3) Preparation of Au@PDA@BCN dispersion: Weigh 1 mg of boron nitride and 2 mg of dopamine prepared in step (2) respectively, and disperse them in Tr...

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Abstract

The invention relates to the field of biology, in particular to a preparation method and a use method of a sandwich type electrochemical immunosensor for detecting a prostate specific antigen. A surface of an electrode is sequentially modified with a polydopamine and boron nitride compound (Au (at) PDA (at) BCN) loaded by gold nanoparticles and having good conductivity and a gold platinum nanoparticle modified manganese dioxide covalent organic framework composite material (AuPt nanoparticles modified manganese dioxide covalent organic framework composite material (AuPt nanoparticles modifiedmanganese dioxide covalent organic framework composite material (AuPt) having an excellent enrichment effect on signal molecule methylene blue (MB). The electrochemical signal amplification effect isachieved through the two materials, and the constructed sandwich type electrochemical immunosensor for detecting the prostate specific antigen by using the peptide and the antibody achieves the purpose of accurately and quantitatively detecting the prostate specific antigen.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for preparing and using a sandwich type electrochemical immunosensor for detecting prostate specific antigen. Background technique [0002] Prostate cancer is one of the main components of male cancer. In the United States, thousands of men die from prostate cancer, so prostate cancer has also received widespread attention from all walks of life. As of now, there is no definitive cure for prostate cancer, and the best way to deal with this problem is a previous diagnosis. Prostate-specific antigen, an important protein associated with prostate cancer in serum, has also been proven to be the most effective marker of prostate cancer. When the concentration of PSA is higher than 4.0ng / mL (the internationally recognized level of PSA in human body), the risk of prostate cancer will be greatly increased. Moreover, the value of PSA also shows the patient's response to prostatectomy sur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/48G01N33/574
CPCG01N27/308G01N27/327G01N27/48G01N33/57407
Inventor 李灿鹏
Owner YUNNAN UNIV
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