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Glioma diagnostic marker Circ19:5604583|5604936 and application

A glioma and marker technology, which is applied in the field of preparation of glioma diagnostic preparations and kits, can solve problems such as lack of understanding of biological mechanisms, and achieve the effect of quickly and accurately grasping the patient's condition and facilitating and accurate diagnosis.

Inactive Publication Date: 2018-01-16
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

This fully reflects our lack of understanding of the biological mechanism of the occurrence and development of glioma, so we cannot effectively intervene in the early stage

Method used

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  • Glioma diagnostic marker Circ19:5604583|5604936 and application
  • Glioma diagnostic marker Circ19:5604583|5604936 and application
  • Glioma diagnostic marker Circ19:5604583|5604936 and application

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Embodiment Construction

[0019] The following embodiments are intended to further illustrate the present invention, rather than limit the present invention.

[0020] 1. Research object

[0021] 1. The two glioma cell lines are U251 and U87 cell lines, and the five primary glioma cell lines are 1216, 1125, 1124B, 1124C, and 0128C, all provided by the Institute of Cancer, School of Basic Medicine, Central South University.

[0022] 2. The serum samples of 40 patients with glioma were provided by Xiangya Hospital, and the serum samples of 15 normal patients were healthy individuals who underwent community disease screening at the same time. The samples used for the research were collected at the same period, and the conditions of sampling, packaging and storage were the same.

[0023] 2. Research methods

[0024] 1. Extraction of RNA from cells, exosomes secreted by cells, and glioma / normal serum exosomes

[0025] A. Extraction of cellular RNA

[0026] After the above-mentioned seven kinds of cells g...

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Abstract

The invention belongs to the technical field of biology and discloses a glioma diagnostic marker Circ19:5604583|5604936 and application. In the invention, exosomes secreted by normal human cells are found to have an obvious enrichment effect on the Circ19:5604583|5604936 for the first time; meanwhile, the expression level of the Circ19:5604583|5604936 in serum exosomes of glioma patients is foundto be remarkably reduced compared with that of a control group (p is equal to 0.0134); ROC (Receiver Operating Curve) analysis shows that the Circ19:5604583|5604936 has higher diagnostic value (AUC isequal to 0.928, p is smaller than 0.0001, and the sensitivity and the specificity are 100 percent and 86.2 percent respectively) to glioma. Therefore, early rapid noninvasive screening and auxiliarydiagnosis of the glioma patients can be realized by detecting the expression level of the Circ19:5604583|5604936 in the serum exosomes in people who possibly suffer from the glioma.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a serum circRNA marker Circ19:5604583|5604936 for the diagnosis of glioma, and the application of a reagent for detecting the marker for preparing a glioma diagnostic preparation, and a kit . Background technique [0002] Glioma is the most common tumor in the central nervous system, accounting for about 40%-50% of all intracranial tumors. Because most gliomas grow infiltratively and have unclear borders with the surrounding brain tissue, even the most modern neurosurgical techniques are difficult to achieve pathological complete resection, so the recurrence rate of gliomas is very high after surgery, and With the increase in the number of operations and recurrence, the degree of malignancy tends to increase. After using modern microsurgical techniques, radiotherapy and chemotherapy and other comprehensive treatment measures, the prognosis of glioma patients is still unsatisfactory, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
Inventor 李沛瑶武明花孟舒娟刘庆袁健赵春花刘洋
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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