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Method for strengthening in-situ remediation of polluted water bodies in rivers and lakes by transgenic plants

A transgenic plant, in situ repair technology, applied in the field of river management and repair, can solve the problems of limited enrichment capacity and stress resistance of aquatic plants

Inactive Publication Date: 2019-10-29
INST OF GEOGRAPHICAL SCI & NATURAL RESOURCE RES CAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that aquatic plants have limited enrichment ability and stress resistance, and have high requirements for water body nutrient content, water flow velocity and other growth environments, this technology uses transgenic plants to repair water bodies

Method used

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  • Method for strengthening in-situ remediation of polluted water bodies in rivers and lakes by transgenic plants
  • Method for strengthening in-situ remediation of polluted water bodies in rivers and lakes by transgenic plants
  • Method for strengthening in-situ remediation of polluted water bodies in rivers and lakes by transgenic plants

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Experimental program
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Effect test

Embodiment 1

[0048] The method for in-situ remediation of polluted water bodies of rivers and lakes enhanced by transgenic plants, wherein the preparation method of transgenic plants is carried out according to the following steps:

[0049] 1. Establishment of plant tissue culture system

[0050] Take wild water peanut plants, wash them with clean water for 5-10 minutes, and cut off the shorter stems with germs and the shorter stems with axillary buds. Sterilize with 75% ethanol for 1 min and 1% NaClO for 8 min on a sterile operating table, wash the sterilized stems with sterile water 6-7 times, remove residual water on sterile filter paper, and then place them on the callus Tissue induction medium (MS+2mg / L2,4D+1mg / L6BA+20mg / L sucrose) and shoot induction medium (1 / 2 MS+2mg / L6BA+0.1mg / LNAA +30mg / L sucrose) culture medium , until it grows into callus and subculture seedlings (such as Figure 1-2 ). The stem segment with axillary buds was asexually propagated in a sterile environment as ...

Embodiment 2

[0062] The method for in-situ remediation of polluted water bodies in rivers and lakes enhanced by transgenic plants, the specific technical scheme is as follows:

[0063] 1. Transfer of nutrients and heavy metal elements in water and sludge

[0064] The water body is rehabilitated by using the transgenic plant water peanut, wherein, the exogenous gene that can enhance the heavy metal pollution efficiency of the plant and the exogenous gene that can improve the tolerance and accumulation of various metals is one of the following genes: ZNT1 gene, and the transgenic plant is One or more of the following plants: water peanut, reed, calamus, water hyacinth, hornwort, canna, water hyacinth, black algae, bitter grass, cress, and rush. Taking water peanut as an example, the optimal initial biomass is 2.6±0.05 g / m3.

[0065] 2. Combined with plant configuration through micro-terrain modification

[0066] Divide polluted river sections and lake landscapes, artificially construct ero...

Embodiment 3

[0071] A method for in-situ remediation of polluted water in rivers and lakes enhanced by transgenic plants:

[0072] 1. Select transgenic plants with basically the same growth, take transgenic Znt1 water peanuts as an example (about 20 cm in height), wash the root soil with distilled water, and move the plants into small black buckets containing polluted water, 3 plants per bucket, small The size of the barrel is 2 L, and each barrel contains 1.8 L of pure water (analytically pure). After 10 days of pre-cultivation, the transgenic plants were subjected to heavy metal treatment experiments with different gradients, Pb 2+ , Zn 2+ 、Cd 2+ The supply was from analytically pure Pb(NO 3 ) 2 , ZnSO 4 ∙7H 2 O and CdCl 2 ∙2.5H 2 O, the treatment without adding heavy metals was used as the control (CK1), and each treatment was repeated 3 times ( figure 1 ). Plants were harvested 2 weeks after treatment. Cut the plants short, wash them, and dry them at 45°C, take 0.2 g of plan...

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Abstract

The invention relates to a method for strengthening in-situ remediation of polluted water bodies in riverways and lakes by transgenic plants. The method is characterized by comprising the steps that 1, in view of the characteristics of difficulty in the growth of conventional plants in moderately and slightly polluted water bodies, eutrophic elements and heavy metal elements in the water bodies are transferred and fixed into bottom sediment by the transgenic plants with high adsorption efficiency and high resistance; and 2, through micro-topography transformation combing with plant configuration, polluted river sections and lake landform landscape are divided, erosion zones and sedimentary zones are artificially constructed, the initial conditions of transgenic plant growth are guaranteed,and the repair efficiency of the polluted river water bodies is improved. The method has the advantages such as high adaptability and environmental friendliness in strengthening the in-situ remediation of the polluted water bodies in the riverways and lakes, and has wide application prospects.

Description

technical field [0001] The invention relates to the field of river channel governance and restoration, in particular to a method for strengthening the in-situ restoration of polluted water bodies of rivers and lakes by using transgenic plants. Background technique [0002] Water is an important resource for the natural environment and agricultural production. At present, many rivers and lakes in my country are facing different degrees of water pollution, especially the imbalance of the aquatic ecosystem caused by the high content of heavy metals in the water. In particular, the discharge of industrial wastewater and domestic sewage causes a large amount of organic matter, P, N elements and heavy metal ions to enter the water body, resulting in the death of plankton and algae, deterioration of water quality, death of fish, shrimp and other organisms, and affecting the ecological cycle. In recent years, with the enhancement of sustainable development, environmental protection,...

Claims

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Application Information

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IPC IPC(8): C02F3/32C12N15/82C12N15/29A01H4/00A01H5/00A01H6/34C02F101/20
CPCC02F3/32C12N15/8205C07K14/415A01H4/00C02F2101/20
Inventor 卢宏玮卢静昭雷凯文康瑜冯玮
Owner INST OF GEOGRAPHICAL SCI & NATURAL RESOURCE RES CAS
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