Preparation method of Cynoglossus semilaevis vibrio anguillarum vaccine

Abstract

The invention discloses a preparation method of a Cynoglossus semilaevis vibrio anguillarum vaccine, which is characterized in that Cynoglossus semilaevis pathogeny Listonella anguillarum BH1 serves as an immunogen, and Astragalus mongholicus lixivium serves as an adjuvant. The preparation method comprises the following steps: inoculating the Cynoglossus semilaevis pathogeny Listonella anguillarum BH1 bacterial strain on a common nutrient broth culture medium; after the bacterial strain is cultured, inactivating by formaldehyde to obtain inactivated bacteria; after dipping the Astragalus mongholicus with distilled water, decocting, and filtering; condensing until the final concentration is 1g of raw drug per ml, and obtaining the Astragalus mongholicus lixivium; and mixing the inactivatedbacteria with the Astragalus mongholicus lixivium to obtain the Cynoglossus semilaevis Vibrio anguillarum vaccine. When the vaccine prepared with the method disclosed by the invention is used for immunizing Cynoglossus semilaevis, the capability of the Cynoglossus semilaevis on resisting Vibrio anguillarum infection can be obviously improved, Vibrio anguillarum infection during the culture production of the Cynoglossus semilaevis can be effectively prevented, the death rate of the Cynoglossus semilaevis is reduced, the culture yield of the Cynoglossus semilaevis is improved, the Cynoglossus semilaevis culture production is guaranteed to healthily and sustainably develop, and the economic benefit for Cynoglossus semilaevis culture production is improved.

Description

technical field [0001] The invention relates to a preparation method of an animal vaccine, in particular to a preparation method of a half-smooth tongue sole vibriosis vaccine; the invention also relates to an immunization method of the vaccine. Background technique [0002] Vibrio anguillarum is an important pathogen that harms aquaculture animals. The Vibriosis caused by it in seawater cultured fish has the characteristics of widespread prevalence, high morbidity and mortality, and is especially harmful to the production of semi-smooth tongue sole. serious. Therefore, the efficient control of vibriosis in eels, in order to minimize its damage, will significantly improve the economic benefits of tongue sole farming. In terms of prevention and control of eel vibriosis in seawater cultured fish, chemically synthesized drugs or antibiotics are mostly used to control the occurrence and prevalence of the disease in aquaculture production, but in the long-term use of drugs, we f...

AI Technical Summary

Problems solved by technology

In terms of prevention and control of eel vibriosis in seawater cultured fish, chemically synthesized drugs or antibiotics are mostly used to control the occurrence and prevalence of the disease in aquaculture production, but in the long-term use of drugs, we found that there are many shortcomings in drug treatment : First, the etiology is complex, there is blindness in a large number of medications, and there is no targeted effective drug for many diseases; second, some chemical drugs have a narrow effective range and weak preventive effect, and diseased fish are often difficult to ingest effective drug doses due to anorexia; It is the drug residues in animals that endanger human health, and long-term drug use brings environmental pollution and leads to the emergence of a large number of drug-resistant microorganisms; in addition, the long-term "protective effect" of drugs makes a large number of disease-resistant Weak individuals survive and gradually increase their proportion in the population, resulting in a decline in the disease resistance of the entire population, which seriously affects the development of the breeding industry

In the prior art, there is no anti-vibriosis vaccine suitable for half-smooth tongue sole

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