Preparation method of Cynoglossus semilaevis vibrio anguillarum vaccine

A technology for semi-smooth tongue sole and Vibrio eel, which is applied in the field of animal vaccine preparation, can solve the problems of narrow effective surface of chemical drugs, emergence of drug-resistant microorganisms, difficulty in ingesting diseased fish, etc. Health and sustainable development, the effect of preventing Vibrio eel infection

Inactive Publication Date: 2011-06-22
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In terms of prevention and control of eel vibriosis in seawater cultured fish, chemically synthesized drugs or antibiotics are mostly used to control the occurrence and prevalence of the disease in aquaculture production, but in the long-term use of drugs, we found that there are many shortcomings in drug treatment : First, the etiology is complex, there is blindness in a large number of medications, and there is no targeted effective drug for many diseases; second, some chemical drugs have a narrow effective range and weak preventive effect, and diseased fish are often difficult to ingest effective drug doses due

Method used

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  • Preparation method of Cynoglossus semilaevis vibrio anguillarum vaccine

Examples

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Embodiment 1

[0017] Example 1. A kind of preparation method of vibriosis vaccine of half-smooth tongue sole, it uses half-smooth tongue sole pathogenic Listonella anguillarum BH1 (Listonella anguillarum BH1) as immunogen, with Radix Astragalus extract as adjuvant; Its preparation method steps are as follows:

[0018] (1) Inoculate Listonia eel BH1 strain on common nutrient broth medium, culture at 30°C for 24h, add formaldehyde with a final volume concentration of 0.3% at 30°C for 48h to obtain inactivated bacteria;

[0019] (2) Soak Astragalus membranaceus in distilled water for 60 minutes, decoct, filter, concentrate to a final concentration of 1 g / mL of the original drug, and obtain Astragalus membranaceus extract;

[0020] (3) Mix the inactivated bacteria with the astragalus extract to make a half-smooth tongue sole vibriosis vaccine; 10 cells.

[0021] When in use, the method of immersion immunization is adopted. First, the immunized half-smooth tongue sole is soaked in an 8% NaCl s...

Embodiment 2

[0022] Example 2. A kind of preparation method of vibriosis vaccine of half-smooth tongue sole, it uses half-smooth tongue sole pathogenic Listonella anguillarum BH1 (Listonella anguillarum BH1) as immunogen, with Radix Astragalus extract as adjuvant; Its preparation method steps are as follows:

[0023] (1) Inoculate Listonia eel BH1 strain on common nutrient broth medium, culture at 28°C for 24h, add formaldehyde with a final volume concentration of 0.3% at 30°C for 48h to obtain inactivated bacteria;

[0024] (2) Soak Astragalus membranaceus in distilled water for 30 minutes, decoct, filter, concentrate to a final concentration of 1 g / mL of the original drug, and obtain Astragalus membranaceus extract;

[0025] (3) Mix the inactivated bacteria with the astragalus extract to make a half-smooth tongue sole vibriosis vaccine; 10 cells.

[0026] When in use, adopt the method of injection immunization, take half-smooth tongue sole with a body length of 15-20cm, and use the imm...

Embodiment 3

[0027] Example 3. A kind of preparation method of vibriosis vaccine of half-smooth tongue sole, it uses half-smooth tongue sole pathogenic Listonella anguillarum BH1 (Listonella anguillarum BH1) as immunogen, with Radix Astragalus extract as adjuvant; Its preparation method steps are as follows:

[0028] (1) Inoculate Listonia eel BH1 strain on common nutrient broth medium, culture at 29°C for 24h, add formaldehyde with a final volume concentration of 0.3% at 29°C for 48h to obtain inactivated bacteria;

[0029] (2) Soak Astragalus membranaceus in distilled water for 45 minutes, decoct, filter, concentrate to a final concentration of 1 g / mL of the original drug, and obtain Astragalus membranaceus extract;

[0030] (3) Mix the inactivated bacteria with the astragalus extract to make a half-smooth tongue sole vibriosis vaccine; 10 cells.

[0031] Oral immunization was adopted during use, and the hard pellet feed was soaked with the semi-smooth tongue sole vibriosis vaccine and...

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Abstract

The invention discloses a preparation method of a Cynoglossus semilaevis vibrio anguillarum vaccine, which is characterized in that Cynoglossus semilaevis pathogeny Listonella anguillarum BH1 serves as an immunogen, and Astragalus mongholicus lixivium serves as an adjuvant. The preparation method comprises the following steps: inoculating the Cynoglossus semilaevis pathogeny Listonella anguillarum BH1 bacterial strain on a common nutrient broth culture medium; after the bacterial strain is cultured, inactivating by formaldehyde to obtain inactivated bacteria; after dipping the Astragalus mongholicus with distilled water, decocting, and filtering; condensing until the final concentration is 1g of raw drug per ml, and obtaining the Astragalus mongholicus lixivium; and mixing the inactivatedbacteria with the Astragalus mongholicus lixivium to obtain the Cynoglossus semilaevis Vibrio anguillarum vaccine. When the vaccine prepared with the method disclosed by the invention is used for immunizing Cynoglossus semilaevis, the capability of the Cynoglossus semilaevis on resisting Vibrio anguillarum infection can be obviously improved, Vibrio anguillarum infection during the culture production of the Cynoglossus semilaevis can be effectively prevented, the death rate of the Cynoglossus semilaevis is reduced, the culture yield of the Cynoglossus semilaevis is improved, the Cynoglossus semilaevis culture production is guaranteed to healthily and sustainably develop, and the economic benefit for Cynoglossus semilaevis culture production is improved.

Description

technical field [0001] The invention relates to a preparation method of an animal vaccine, in particular to a preparation method of a half-smooth tongue sole vibriosis vaccine; the invention also relates to an immunization method of the vaccine. Background technique [0002] Vibrio anguillarum is an important pathogen that harms aquaculture animals. The Vibriosis caused by it in seawater cultured fish has the characteristics of widespread prevalence, high morbidity and mortality, and is especially harmful to the production of semi-smooth tongue sole. serious. Therefore, the efficient control of vibriosis in eels, in order to minimize its damage, will significantly improve the economic benefits of tongue sole farming. In terms of prevention and control of eel vibriosis in seawater cultured fish, chemically synthesized drugs or antibiotics are mostly used to control the occurrence and prevalence of the disease in aquaculture production, but in the long-term use of drugs, we f...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/106A61P31/04
Inventor 阎斌伦张晓君
Owner HUAIHAI INST OF TECH
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