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Preparation method of compound microbial flocculant for cyanobacterial bloom

A microbial flocculant and cyanobacteria bloom technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of less research on eutrophication of water bodies, achieve good application prospects, high yield, and easy Inactivation effect

Inactive Publication Date: 2011-10-19
DONGHUA UNIV
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AI Technical Summary

Problems solved by technology

[0004] Microbial flocculants have different flocculation effects on different suspended solids. At present, there are few studies on the use of microbial flocculants for water eutrophication.

Method used

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  • Preparation method of compound microbial flocculant for cyanobacterial bloom
  • Preparation method of compound microbial flocculant for cyanobacterial bloom
  • Preparation method of compound microbial flocculant for cyanobacterial bloom

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Embodiment 1

[0028] Determination method of flocculation: add 0.4g kaolin to a 150mL beaker, add 80ml distilled water, adjust the pH to 4.5, add 4mL of 1% CaCl 2 Solution and 1.5mL complex flora fermentation broth, then add distilled water to 100mL. Stir for 2 minutes with a constant temperature magnetic stirrer at 200 r / min at room temperature, and let stand for 5 minutes at room temperature. Draw the supernatant at 50mL, measure the optical density value ODi at a wavelength of 550nm with a UV-visible spectrophotometer (type 752), and replace the complex flora fermentation broth with 2mL of uninoculated fresh fermentation medium, and other operating conditions are complete. The same experiment was used as a control, and the optical density value OD was measured 0 .

[0029] The formula for calculating the flocculation rate is:

[0030]

[0031] Screening and identification of efficient flocculation bacteria:

[0032] (1) The strains were screened from the activated sludge, and the ...

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Abstract

The invention relates to a preparation method of a compound microbial flocculant for cyanobacterial bloom. The preparation method comprises the following steps: respectively inoculating Serratia and Bacillus into a sterilized nutrient broth medium for culture; respectively taking out 1mL of bacterial liquids of Serratia and Bacillus after culturing for 24h, and inoculating into a sterilized fermentation medium for fermentation culture; treating a broth as a seed liquid after culturing for 72 h and carrying out complex culture for 72h under conditions of a culture temperature of 35 DEG C, a rotation speed of 160rpm, an initial pH of a medium of 8.0, and an inoculation amount of the seed liquid of 10 ml / L; and carrying out centrifugal separation on the broth, and taking out a supernatant liquor. So the compound microbial flocculant is obtained. The preparation method is simple, fast and low cost, the flocculant prepared in the invention has the advantages of small dosage in use, strong stability and easy storage, can effectively flocculate cyanobactreial water samples with a flocculation rate of 92%, and has an excellent application prospect.

Description

technical field [0001] The invention belongs to the field of preparation of composite microbial flocculants, in particular to a preparation method of composite microbial flocculants for cyanobacteria blooms. Background technique [0002] Flocculants are widely used in industrial fields such as sewage treatment, food production, and biological fermentation. However, the residues of inorganic and organic polymer flocculants that are widely used at present usually cause environmental pollution problems, and even have carcinogenic and teratogenic toxicity to humans. It has been banned or used in limited quantities in many countries, so it is necessary to develop a new type of flocculant that is efficient, safe, non-toxic, and does not pollute the environment. [0003] Bioflocculants are mainly high molecular biopolymers with flocculation activity secreted by microorganisms under specific culture conditions. They are safe, non-toxic, and environmentally friendly. At present, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P1/04C12R1/425C12R1/07
Inventor 赵晓祥夏莉莉
Owner DONGHUA UNIV
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