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Inoculant for degrading aflatoxin B1 and applications thereof

A technology of aflatoxin and bacterial agent, applied in the direction of bacteria, microbe-based methods, microbes, etc., can solve the problems of poor effectiveness, high cost, and lack of popularization and application, and achieve the effect of simple and easy operation in the preparation process

Inactive Publication Date: 2017-03-29
SHENYANG NORMAL UNIV
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  • Abstract
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Problems solved by technology

Various physical and chemical methods to reduce aflatoxin B1 in grain and food have been proved to be very ineffective and costly, and have no practical application

Method used

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  • Inoculant for degrading aflatoxin B1 and applications thereof
  • Inoculant for degrading aflatoxin B1 and applications thereof

Examples

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preparation example Construction

[0014] The preparation method of the bacterial agent for degrading aflatoxin B1:

[0015] The Brevibacterium halotolerant JH8 (Shanghai Fuxiang Biotechnology Co., Ltd.) isolated or purchased from the soil was cultivated in nutrient broth NB520 (commercially purchased), and the cultivation was carried out in a shaker incubator, and the cultivation condition was that the speed was 150 rpm. Minutes, at a temperature of 30°C, and cultured for 18 hours to prepare a spray-type microbial agent.

[0016] Brevibacterium halotolerant JH8 is separated from the soil by the following method: take 2 grams of soil 12 to 15 centimeters below the surface of the soil layer, dissolve it in 20 milliliters of 0.9% sterile saline, shake it up, and centrifuge at 400 rpm for 90 minutes, take 100 μl of supernatant was spread on sterilized nutrient agar medium, and cultured at 30°C for 72 hours. A single colony was picked and repeatedly purified and cultured to obtain Brevibacterium halotolerant JH8. ...

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Abstract

The invention belongs to the technical field of microbial functional inoculants, and specifically relates to an inoculant for degrading aflatoxin B1 and applications thereof. The inoculant is prepared through amplification culture of brevibacterium halotolerans JH8 in nutrient broth. The amplification culture is performed in a shaking table incubator, the culture conditions are: the rotation speed is 150 to 170 r / min, the temperature is 30 to 33 DEG C, and the culture time is 18 to 24 hours. The provided inoculant can degrade aflatoxin B1 in grains until no aflatoxin B1 is detected by a kit. The brevibacterium halotolerans JH8 is cheap and easily available. The preparation method is simple and easy to operate. Moreover, the inoculant is pollution-free to the grains.

Description

technical field [0001] The invention belongs to the technical field of microbial functional bacterial agents, and in particular relates to a microbial agent for degrading aflatoxin B1 and its application. Background technique [0002] The International Agency for Research on Cancer reported in 2000 that aflatoxins are a group of toxins produced by Aspergillus flavus that can cause cardiovascular diseases in humans, among which aflatoxin B1 is the most toxic and can cause liver diseases in humans. Due to the toxicity of aflatoxin, the European Union and some other countries in the world have set the maximum limit of aflatoxin B1 in food and food in the hazard analysis and critical control point (HACCP) to ensure that human healthy diet. Grain and food trade between countries have also passed legislation to limit the content of aflatoxin B1 (EU Reg EC165 / 2010). At the same time, the 2010 annual report of the European Food and Feed Grain Early Warning System pointed out that ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L5/20C12R1/13
CPCC12N1/20
Inventor 乔国华肖志刚李桂杰解铁民
Owner SHENYANG NORMAL UNIV
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