40results about How to "Broad antimicrobial activity" patented technology

The invention discloses a small molecule peptide with antibacterial antiviral activity and an active modifier of the small molecule peptide. An amino acid sequence of the small molecule peptide is formed by 3-7 amino acids; a general formula of the amino acid sequence is X[0-3](R/K/O/D[ab])X[0-1]W[1-2](R/K/O/D[ab])[0-1]X[0-1], wherein X is one of the amino acids R, K, W and I; X[0-3] is any 0-3 of R, K, W and I; X[0-1] is any 0-1 of R, K, W and I; W[1-2] represents one or two Ws; (R/K/O/D[ab])[0-1] represents zero or one amino acid R or K or O or Dab; D[ab] is a 2,4-diamido-butyric acid. The experiment proves that the small molecule peptide or the active modifier thereof disclosed by the invention has good antibacterial antiviral effects, and has important application value in preparation of a drug, a sanitizer, a detergent, a corrosion remover or a packaging material for removing bacteria, fungi or viruses.

The invention relates to a quick preparation method and application of chickpea polypeptide powder. The method comprises the following steps: by using chickpea beans or dry chickpea slag obtained by extracting small molecule compounds with an organic solvent as the raw material, pulverizing, degreasing, carrying out a modern ultrafiltration membrane technique, and carrying out modern spray drying or freeze-drying to obtain the chickpea polypeptide powder. The chickpea polypeptide powder proves to have antibacterial and anti-fungus activities, has wide antimicrobial activity, antioxidation activity and blood sugar reduction activity, has the functions of enhancing humoral immunity and general immunity, and contains rich pure natural plant-derived polypeptides and proteins which can be easily digested and absorbed by the human body. The chickpea polypeptide powder maintains the chickpea polypeptide proteins, and achieves the goals of changing wastes into valuable substances and fully utilizing resources. The chickpea polypeptide powder is used as a natural antimicrobial agent, food additive or drug, and can be used in food and medicine industries.

The invention discloses a copper metal organic framework nanoparticle functionalized hydrogel, a preparation method and application thereof. The preparation method comprises the following steps: dissolving carboxymethyl chitosan, and adding reduced glutathione and N-hydroxysuccinimide which are mixed and dissolved in deionized water; adding 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride to prepare glutathione modified carboxymethyl chitosan; preparing copper metal organic framework nanoparticles; then mixing the copper metal organic framework nanoparticles with deionized water; and adding the glutathione modified carboxymethyl chitosan, acrylamide, polyethylene glycol diacrylate, polyethylene glycol diglycidyl ether and an initiator to prepare the copper metal organic framework functionalized hydrogel. According to the invention, the obtained hydrogel has the capabilities of slowly and controllably releasing copper ions, removing active oxygen of a wound surface, always keeping the wound surface free of hydrops and a wet 3D microenvironment, protecting the wound from external injury and promoting wound healing.

The invention relates to a rana nigromaculata antibacterial peptide and a gene and application thereof, belonging to the technical field of medical technology. The rana nigromaculata antibacterial peptide is a single-chain polypeptide coded by a gene of a rana nigromaculata amphibious animal in China and has molecular weight of 1495.85D and isoelectric point of 9.0; and complete sequence of the rana nigromaculata antibacterial peptide is valine-isoleucine-proline-isoleucine-valine-serin-glycine-leucine-leucine-serin-serin-leucine-leucine-glycine-lysine. A coding gene of the rana nigromaculata antibacterial peptide is composed of 333 nucleotides, wherein nucleotides No 142-186 are used for coding a mature part. The artificially synthesized rana nigromaculata antibacterial peptide has strong antibacterial action and high safety, also has the advantages of simple sequence and convenience for synthesis and can be applied to a novel antiinfection medicine.

The invention discloses a plant lactobacillus pln E and pln The heterologous expression method of F, found from NCBI pln E and pln The gene sequence of F, the sequence number is, and an enterokinase site is added at the N-terminal of the sequence, and an enzyme cutting site is designed Kpn I and Nco I, synthetic sequence; plasmid pUC57- pln E and pUC57- pln F is the template, and primers are designed for pln E and pln PCR amplification of the F fragment, after double digestion of the pET-32a plasmid and the target fragment, the recombinant plasmid pET-32a- pln E and pET-32a- pln F, transformed into E. coli E. coli DH5α and save. The invention utilizes the heterologous expression system of Escherichia coli to construct an expression plasmid and realize its high-efficiency expression in Escherichia coli, making it possible to prepare a large amount of PlnE/F, thereby providing a basis for the study of its mechanism of action and its future application in the field of food.

The invention discloses a stable urine microalbumin quality control material and a preparation method thereof. The stable urine microalbumin quality control material solves the technical problem thatthe existing urine microalbumin quality control material easily produces attenuation and cannot be preserved for a long time. The stable urine microalbumin quality control material belongs to the technical field of medical device biochemical in-vitro diagnosis. The stable urine microalbumin quality control material comprises 1-50mmol/L of a buffer with pH of 7.2-7.4, 5-15g/L of salt, 0.01-0.1% ofa protective agent A, 1-10% of a protective agent B, 1-10mL/L of a surfactant and 8.67-21.33mg/L or 45.2-62.8mg/L of albumin. The stable urine microalbumin quality control material has broad-spectrumantibacterial activity, has a wide application range, prevents microbial damage to quality control products, prevents protein attenuation, improves product stability and has an effect period of 24 months.

The invention relates to a preparation method of half pea polypeptide of chickpea and application thereof. The preparation method comprises the following steps of fully degreasing the grease componentin the pea half of the chickpea, extracting by a phosphate buffer solution, hydrolyzing by neutral protease, alkaline protease, trypsin or papain, and adopting an ultrafiltration membrane technique to prepare the polypeptide with molecular weight of 6 to 10kDa, 3.5 to 6kDa and 1 to 3.5kDa; spray-drying, or freeze-drying, so as to obtain the half pea polypeptide. The polypeptide powder has the advantages that the bacteria and fungi-resistant activity is realized, the broad microorganism-resistant activity is realized, the function of enhancing the immunity of body liquid and whole body is realized, and the polypeptide and protein from pure natural plants which can be easily digested and absorbed by a human body are contained. The protein has the characteristics that the original biologicalactivity of the chickpea is reserved, the waste is changed into the valuable, and the resources can be fully utilized; the protein is used as a natural antibacterial agent, a food additive or a medicine, and can be applied to food and medicine industries.

The invention relates to a method for increasing yield of antifungal peptide bacillomycin D by over-expression of spo0A gene and belongs to the field of biotechnology. In the method, firstly, with CGMCC No.0943 Bacillus subtilis fmbJ being an initial strain, a gene over-expression carrier pCBS-LPSpo0AR is constructed with Escherichia coli-Bacillus subtilis shuttle cloning carrier pMAD being a framework, and by means of the principle of homologous recombination, a strain, fmbJspo0A, which is high in yield of bacillomycin D, is constructed in a manner of knocking the spo0A gene of the fmbJ genome of the Bacillus subtilis in an amylase locus through a double exchange process. A high-performance liquid chromatographic test proves that the capability of producing the bacillomycin D by the improved bacterial strain is increased by 1.82 times.

The invention discloses a preparation method of a titanium dioxide nano composite material, and belongs to the technical field of nano medical materials. The invention provides a medical composite material with wide antiviral activity, antibacterial activity and anticancer activity, and provides the preparation method of the titanium dioxide nano composite material. According to the invention, titanium dioxide is used as an inorganic semiconductor nano material as a carrier, encapsulation of organic photosensitive molecules is carried out, and biocompatible macromolecules are used for surfacemodification. The prepared material has wide antiviral activity, antibacterial activity and anticancer activity, and the biological activity of the material destroys viruses, bacteria and nucleic acids, proteins, lysosomes and mitochondria of bacterial and infected cells based on active oxygen generated by photocatalysis, so that the viruses, bacteria and cancer cells cannot be copied, and tissuenutrients cannot be supplied, thereby killing the viruses, bacteria and cancer cells. The nano composite material provided by the invention can also be used as a protective material for medical treatment and public health, including medical masks, protective clothing, gloves, disinfectants and the like.

The invention provides a method for increasing yield of antifungal peptide bacillomycin D by overexpressing a degU gene and belongs to the technical field of biology. The method comprises steps as follows: firstly, a gene overexpression vector pCBS-LPDegUR is constructed by bacillus subtilis fmbJ with preservation number being CGMCC No.0943 as an original strain and an escherichia coli-bacillus subtilis shuttle cloning vector pMAD as a skeleton, and by means of the homologous recombination principle, a strain fmbJdegU with high bacillomycin D yield is constructed with a method for knocking theDegU gene of bacillus subtilis fmbJ genome into an amylase site in a double exchange process. High performance liquid chromatography determines that capacity of the degU gene overexpressing strain for producing bacillomycin D is improved substantially.

The invention relates to a rana nigromaculata antimicrobial peptide as well as a gene and application thereof, and belongs to the technical field of biomedicine. The rana nigromaculata antimicrobial peptide is a gene coded single-chain polypeptide separated from an amphibian rana nigromaculata of China, wherein the molecular weight is 1,647.02 Daltons, and the isoelectric point is 11.05; and the complete sequence of the rana nigromaculata antimicrobial peptide is phenylalanine-isoleucine-proline-leucine-valine-serine-glycine-leucine-phenylalanine-serine-arginine-leucine-leucine-glycine-lysine. The coded gene of the rana nigromaculata antimicrobial peptide consists of 316 nucleotides, wherein the mature coded part of the coded gene contains 142nd-186th nucleotides. The artificially synthesized rana nigromaculata antimicrobial peptide has a strong antibacterial effect, has the advantages of high safety, simple sequence and convenience in synthesis, and can be used as a novel anti-infective agent for application.

The invention relates to a bone marrow protein and polypeptide ultrafiltration membrane preparation method and an application thereof. Fresh bone marrow in animal bones in a slaughter house for cattle, sheep, horses, camels or other animals serves as a raw material, the bone marrow and bones are classified, cleaned with clear water, frozen by liquid nitrogen and crushed to obtain bone marrow powder, warm water solution is used for extraction, grease components are sufficiently degreased, and the protein with the molecular weight cut-off of 10KDa is prepared by modern ultrafiltration membrane technology and then refined by modern spray drying technology or freeze-drying. The antibacterial and antifungal activity of the bone marrow protein is verified, and the bone marrow protein has wide antimicrobial activity, has the function of enhancing humoral immunity and general immunity and is rich in human easily digestible and absorbable pure natural plant source polypeptide and protein. The protein has the advantages that original protein of the bone marrow is retained, waste is turned into wealth, resources are sufficiently used, and the protein serving as a natural antibacterial agent, a food additive or a medicine can be used for food and pharmaceutical industries.

The invention relates to a method for increasing antifungal peptide bacillomycin D yield by expressing comA gene and belongs to the technical field biology. The method is characterized in that CGMCC No. 0943 bacillus subtilis fmbJ is used as the original strain and escherichia coli-bacillus subtilis shuttle cloning vector pMAD is used as the framework to build a gene overexpression vector pCBS-LPComAR, and a high-bacillomycin-D-yield strain named fmbJcomA is built by knocking in the bacillus subtilis fmbJ genome comA gene at an amylase site during double exchange according to the homologous recombination principle. High-performance liquid chromatography determines that the bacillomycin D production capacity of the improved strain is increased greatly.

The invention discloses Streptomyces Phaeochromogenes SICAU417 and an application thereof. The strain is preserved in the China Center for Type Culture Collection (CCTCC), the address is Luojia hill,Bayi road, Wuchang district, Wuhan city, Hubei province, the preservation date is October 8, 2018, and the preservation number is CCTCC NO:M 2018661. The 16S rRNA sequence of the Streptomyces Phaeochromogenes SICAU417 is shown as in SEQ ID NO.1. The Streptomyces Phaeochromogenes SICAU417 has a wider antibacterial action, promotes the plant growth at the same time, and has the double-effect functions of both a fertilizer and a pesticide, and a microbial fertilizer prepared by using the strain is of great significance for reducing the chemical fertilizer and applying the pesticide in the agricultural planting industry.

An antibacterial hand cream is composed of the components by the mass percentage: 0.1% to 0.5% of chlorhexidine gluconate, 2% to 10% of glycerin, 3% to 10% of olive oil, 3% to 12% of cetostearyl alcohol, 5% to 10% of PEG-100 stearic acid ester, 1% to 5% of polydimethylsiloxane, 0.2% to 0.5% of a chamomile extract, 0.05% to 0.09% of an essence and the balance purified water. The used chamomile extract is rich in apigenin, and the apigenin can be used as an antiviral drug for virus infection for treating all kinds of inflammation and has low toxicity and no mutagenicity as compared with other flavonoids; chlorhexidine gluconate is biguanide chlorobenzene, has considerable wide antimicrobial activity, is the most effective against Gram-positive and Gram-negative bacteria at the pH of 5.0-8.0,has broad-spectrum and long-acting antibacterial effects, is non-toxic, odorless and non-irritating, and can be loved by people when being used in the antibacterial hand cream.

The invention discloses a camellia oil antisepsis and anti-inflammation gel, which is mainly prepared from camellia oil, radix arnebiae extracts, aloe gel freeze-dried powders and green tea extracts.Accordingly, an inventor also establishes a corresponding preparation method. Researches indicate that the camellia oil antisepsis and anti-inflammation gel prepared from the extraction solutions of various medicines has the functions of resisting bacteria, diminishing inflammation, resisting eczema, quickening skin tissues to be repaired and the like, has a good function for alleviating, repairing and preventing infant diaper rashes, and can effectively eliminate infant diaper rashes and prevent diaper dermatitis, even infantile eczema and the like. In addition, the camellia oil antisepsis and anti-inflammation gel has a simple product preparation technology, moderate product properties, good stability and high safety, is clear, moist and nutrient, has a pleasant smell, is convenient to use, and is suitable in the four seasons.