A kind of preparation method and application of chickpea watercress polypeptide
A technology of chickpea watercress and chickpea, which is applied to the preparation method of peptides, chemical instruments and methods, and medical preparations containing active ingredients, etc., which can solve the problems of waste of rare and unique plant resources, etc.
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Examples
Embodiment 1
[0034] a. Grind 613g of dry watercress after germinating and peeling chickpeas, pass through a 80-mesh sieve, add petroleum ether according to w / v soybean powder: petroleum ether = 1:5, shake at room temperature for 4 hours, and repeatedly extract 3-4 times Until it is colorless, the chickpea watercress powder is naturally settled and separated from petroleum ether, and the upper layer of petroleum ether is poured out for recovery. The precipitates are combined and dried naturally to obtain 516.7g of defatted chickpea watercress powder with an oil extraction rate of 96.3 / 613=15.7%, put it in a -20℃ refrigerator and store it for later use;
[0035] b. Add 350ml of phosphate buffer solution with a pH of 7.4 and 0.1M to 100g of degreased chickpea watercress powder in step a, the ratio of solid to liquid is 1:3.5, the temperature is 4°C, and the extraction is carried out for 48h;
[0036] c. The extract in step b was filtered, 10000r / min, temperature 4°C, centrifuged for 10min, t...
Embodiment 2
[0042] a. Grind 613g of dry watercress after germinating and peeling chickpeas, pass through a 80-mesh sieve, add petroleum ether according to w / v soybean powder: petroleum ether = 1:5, shake at room temperature for 4 hours, and repeatedly extract 3-4 times Until it is colorless, the chickpea watercress powder is naturally settled and separated from petroleum ether, and the upper layer of petroleum ether is poured out for recovery. The precipitates are combined and dried naturally to obtain 516.7g of defatted chickpea watercress powder with an oil extraction rate of 96.3 / 613=15.7%, put it in a -20℃ refrigerator and store it for later use;
[0043] b. Add 350ml of phosphate buffer solution with a pH of 7.4 and 0.1M to 100g of degreased chickpea watercress powder in step a, the ratio of solid to liquid is 1:3.5, the temperature is 4°C, and the extraction is carried out for 48h;
[0044] c. The extract in step b was filtered, 10000r / min, temperature 4°C, centrifuged for 10min, t...
Embodiment 3
[0050] a. Grind 613g of dry watercress after germinating and peeling chickpeas, pass through a 80-mesh sieve, add petroleum ether according to w / v soybean flour: petroleum ether = 1:5, shake at room temperature for 4 hours, and repeatedly extract 3-4 times Until it is colorless, chickpea watercress powder naturally settles and separates from petroleum ether, pours out the upper layer of petroleum ether for recovery, combines the precipitates, and naturally dries to obtain 516.7g of degreased chickpea watercress powder with an oil extraction rate of 96.3 / 613=15.7%, put it in a -20℃ refrigerator and store it for later use;
[0051] b. Add 350ml of phosphate buffer solution with a pH of 7.4 and 0.1M to 100g of degreased chickpea watercress powder in step a, the ratio of solid to liquid is 1:3.5, the temperature is 4°C, and the extraction is carried out for 48h;
[0052]c. The extract in step b was filtered, 10000r / min, temperature 4°C, centrifuged for 10min, temperature 4°C, dia...
PUM
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Abstract
Description
Claims
Application Information
- IPC
- C07K1/34; C12P21/06; A23L33/185; A23L33/18; A61K38/01; A61P31/04; A61P31/10
- CPC
- A61K38/011; A23L33/18; A23L33/185; C07K1/34; C12P21/06
- Inventors
- 阿布力米提·伊力; 艾合米丁·外力