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Bone marrow protein and polypeptide ultrafiltration membrane preparation method and application thereof

A technology of protein and ultrafiltration membrane, which is applied in the preparation of ultrafiltration membrane of bone marrow protein and polypeptide and its application field. It can solve the problems of environmental pollution, waste, and non-utilization of livestock and poultry bones, and achieve good application prospects.

Active Publication Date: 2017-06-30
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country is a big consumer of livestock and poultry, but most of them focus on the consumption of livestock and poultry meat. "A large number of livestock and poultry bones cannot be used", which not only wastes this resource with rich nutrients and a reasonable proportion, but also pollutes the issue of bone treatment. environmental pressure

Method used

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  • Bone marrow protein and polypeptide ultrafiltration membrane preparation method and application thereof
  • Bone marrow protein and polypeptide ultrafiltration membrane preparation method and application thereof
  • Bone marrow protein and polypeptide ultrafiltration membrane preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] a. Take 100g of fresh bone marrow, wash it 3 times, remove the smashed bones and blood stains, pre-cool, chop into small pieces, add liquid nitrogen at a weight ratio of 1:5, and grind them repeatedly for 3-4 times to obtain bone marrow crushed powder , stored in a low-temperature refrigerator for later use;

[0035] b. Add distilled water with a weight ratio of 1:10 to the crushed bone marrow sample in step a, place it on a magnetic stirrer for extraction for 2 hours at a temperature of 30°C, extract twice each time, filter under reduced pressure, combine the extracts, and separate impurities to obtain the extract for subsequent use;

[0036] c. Put the extract obtained in step b at a temperature of 4°C overnight to separate the oil-water layer, take out the upper oil layer, put the lower extract layer in a separatory funnel, add petroleum ether according to the volume ratio of material to liquid at 1:5, and keep shaking Carry out degreasing, stand still for 3h until ...

Embodiment 2

[0041] a. Take 100g of fresh bone marrow, wash it 3 times to remove the smashed bones and blood, chop up small pieces, add liquid nitrogen to crush at a weight ratio of 1:5, and repeatedly crush for 3-4 times to obtain crushed bone marrow powder. Store in the refrigerator for later use;

[0042] b. Add distilled water with a weight ratio of 1:10 to the crushed bone marrow powder in step a, place it on a magnetic stirrer for extraction for 4 hours at a temperature of 40°C, extract 3 times each time, filter under reduced pressure, combine the extracts, and separate impurities. Obtain the extract for subsequent use;

[0043] c. Put the extract obtained in step b at a temperature of 4°C overnight to separate the oil-water layer, take out the upper oil layer, put the lower extract layer in a separatory funnel, add n-hexane according to the volume ratio of material to liquid at 1:5, and keep shaking Carry out degreasing, stand still for 5h until no oily matter is seen in the upper ...

Embodiment 3

[0048] a. Take 100g of fresh bone marrow, wash it 3 times to remove the smashed bone and blood, chop into small pieces, add liquid nitrogen to grind at a weight ratio of 1:5, repeat grinding 3 times to get bone marrow powder, and store in a low-temperature refrigerator spare;

[0049] b. Add distilled water with a weight ratio of 1:10 to the crushed bone marrow powder in step a, place it on a magnetic stirrer for extraction for 4 hours at a temperature of 35°C, extract twice each time, filter under reduced pressure, combine the extracts, and separate impurities. Obtain the extract for subsequent use;

[0050] c. Put the extract obtained in step b at a temperature of 4°C overnight to separate the oil-water layer, take out the upper oil layer, put the lower extract layer in a separatory funnel, add n-hexane according to the volume ratio of material to liquid at 1:5, and keep shaking Carry out degreasing, stand still for 4h until no oily matter is seen in the upper layer, and ob...

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Abstract

The invention relates to a bone marrow protein and polypeptide ultrafiltration membrane preparation method and an application thereof. Fresh bone marrow in animal bones in a slaughter house for cattle, sheep, horses, camels or other animals serves as a raw material, the bone marrow and bones are classified, cleaned with clear water, frozen by liquid nitrogen and crushed to obtain bone marrow powder, warm water solution is used for extraction, grease components are sufficiently degreased, and the protein with the molecular weight cut-off of 10KDa is prepared by modern ultrafiltration membrane technology and then refined by modern spray drying technology or freeze-drying. The antibacterial and antifungal activity of the bone marrow protein is verified, and the bone marrow protein has wide antimicrobial activity, has the function of enhancing humoral immunity and general immunity and is rich in human easily digestible and absorbable pure natural plant source polypeptide and protein. The protein has the advantages that original protein of the bone marrow is retained, waste is turned into wealth, resources are sufficiently used, and the protein serving as a natural antibacterial agent, a food additive or a medicine can be used for food and pharmaceutical industries.

Description

technical field [0001] The invention relates to a method for preparing an ultrafiltration membrane of bone marrow protein and polypeptide and its application. It is a protein with antibacterial activity prepared by using the bone with abundant resources in Xinjiang as a raw material. It has biological activities such as antibacterial and antifungal. It is used in medicine, food The field has a good application prospect. Background technique [0002] my country is a big consumer of livestock and poultry, but most of them focus on the consumption of livestock and poultry meat. "A large number of livestock and poultry bones cannot be used", which not only wastes this resource with rich nutrients and a reasonable proportion, but also pollutes the issue of bone treatment. "environmental pressure" caused certain environmental pressure. [0003] The utilization of protein in livestock and poultry bones, bone protein, the protein content in bone is very high, and it is a relatively ...

Claims

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Application Information

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IPC IPC(8): C07K1/34A61K38/02A61P31/04A61P31/10A23L33/18
CPCA61K38/012C07K1/34
Inventor 阿布力米提·伊力阿吉艾克拜尔·艾萨帕尔哈提·柔孜雅森·米吉提艾合米丁·外力
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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