A preparation method and application of bone marrow protein and polypeptide

A protein and bone marrow technology, applied in the field of bone marrow protein and polypeptide preparation, can solve problems such as waste, environmental pollution, and non-utilization of livestock and poultry bones

Active Publication Date: 2020-08-14
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country is a big consumer of livestock and poultry, but most of them focus on the consumption of livestock and poultry meat. "A large number of livestock and poultry bones cannot be used", which not only wastes this resource with rich nutrients and a reasonable proportion, but also pollutes the issue of bone treatment. environmental pressure

Method used

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  • A preparation method and application of bone marrow protein and polypeptide
  • A preparation method and application of bone marrow protein and polypeptide
  • A preparation method and application of bone marrow protein and polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] a. Take 100g of fresh bone marrow, wash 3 times, remove broken bones and blood stains, pre-cool, chop into small pieces, add liquid nitrogen to crush at a weight ratio of 1:5, and crush 3-4 times repeatedly to obtain crushed bone marrow powder , Keep in low temperature refrigerator for later use;

[0035] b. Add distilled water with a weight ratio of 1:10 to the bone marrow sample that has been pulverized in step a, and set it on a magnetic stirrer for 2 hours at a temperature of 30°C. Extract twice at a time, filter under reduced pressure, combine the extracts, and separate impurities And get the extract for use;

[0036] c. Put the extract obtained in step b at 4°C overnight to separate the oil and water layers, take out the upper oil layer, place the lower extract layer in a separatory funnel, add petroleum ether according to the volume ratio of material to liquid of 1:5, and shake it constantly Carry out degreasing, stand for 3 hours until no oily matter is seen in the ...

Embodiment 2

[0041] a. Take 100g of fresh bone marrow and wash it 3 times to remove the broken bones and blood stains, cut into small pieces, add liquid nitrogen in a weight ratio of 1:5, and pulverize repeatedly 3-4 times to obtain bone marrow powder, low temperature Keep in the refrigerator for later use;

[0042] b. Add distilled water with a weight ratio of 1:10 to the bone marrow crushed powder in step a, and place it on a magnetic stirrer for 4 hours, at a temperature of 40°C, extract 3 times each time, filter under reduced pressure, combine the extracts, and separate impurities. Obtain the extract for use;

[0043] c. Put the extract obtained in step b at 4°C overnight to separate the oil and water layers, take out the upper oil layer, place the lower extract layer in a separatory funnel, add n-hexane according to the volume ratio of the material to liquid at 1:5, keep shaking Carry out degreasing, stand still for 5 hours until no oil-free matter is seen in the upper layer, and obtain a...

Embodiment 3

[0048] a. Take 100g of fresh bone marrow and wash it 3 times to remove the broken bones and blood stains, cut into small pieces, add liquid nitrogen at a weight ratio of 1:5, and smash it repeatedly for 3 times to obtain bone marrow powder, and store in a low-temperature refrigerator spare;

[0049] b. Add distilled water with a weight ratio of 1:10 to step a bone marrow crushed powder, set it on a magnetic stirrer for 4 hours, at a temperature of 35°C, extract twice each time, filter under reduced pressure, combine the extracts, and separate impurities. Obtain the extract for use;

[0050] c. Put the extract obtained in step b at 4°C overnight to separate the oil and water layers, take out the upper oil layer, place the lower extract layer in a separatory funnel, add n-hexane according to the volume ratio of the material to liquid at 1:5, keep shaking Carry out degreasing, stand for 4h until no oily matter is seen in the upper layer, and obtain a defatted crude extract;

[0051] d...

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Abstract

The invention relates to a bone marrow protein and polypeptide ultrafiltration membrane preparation method and an application thereof. Fresh bone marrow in animal bones in a slaughter house for cattle, sheep, horses, camels or other animals serves as a raw material, the bone marrow and bones are classified, cleaned with clear water, frozen by liquid nitrogen and crushed to obtain bone marrow powder, warm water solution is used for extraction, grease components are sufficiently degreased, and the protein with the molecular weight cut-off of 10KDa is prepared by modern ultrafiltration membrane technology and then refined by modern spray drying technology or freeze-drying. The antibacterial and antifungal activity of the bone marrow protein is verified, and the bone marrow protein has wide antimicrobial activity, has the function of enhancing humoral immunity and general immunity and is rich in human easily digestible and absorbable pure natural plant source polypeptide and protein. The protein has the advantages that original protein of the bone marrow is retained, waste is turned into wealth, resources are sufficiently used, and the protein serving as a natural antibacterial agent, a food additive or a medicine can be used for food and pharmaceutical industries.

Description

Technical field [0001] The invention relates to a method for preparing bone marrow protein and polypeptide and its application. It is a protein with antibacterial activity prepared by using Xinjiang's resource-rich bones as raw materials. It has antibacterial, antifungal and other biological activities, and is very useful in the fields of medicine and food. Good application prospects. Background technique [0002] my country is a big country of livestock and poultry consumption, but it is mostly concentrated on the consumption of livestock and poultry meat. "A large amount of livestock and poultry bones cannot be used" not only wastes this nutrient-rich and reasonable ratio resource", but also pollutes the problem of bone treatment. The "environment" caused a certain environmental pressure. [0003] The utilization of protein in the bones of livestock and poultry, bone protein, the protein content in bone is very high, and it is a relatively complete soluble protein, and the biolog...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/34A61K38/02A61P31/04A61P31/10A23L33/18
CPCA61K38/012C07K1/34
Inventor 阿布力米提·伊力阿吉艾克拜尔·艾萨帕尔哈提·柔孜雅森·米吉提艾合米丁·外力
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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