Streptomyces Phaeochromogenes SICAU417 and application thereof

A technology of Streptomyces chromogenes and dark color, which is applied in the field of microorganisms, can solve the problems of irreversible damage to the ecological environment, and achieve the effects of increasing plant height and leaf area, inhibiting growth, and promoting growth

Active Publication Date: 2019-03-08
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the application of traditional pesticides and chemical fertilizers can increase the yield of highland barley in a short period of time and alleviate the contradiction between supply and demand, it will cause irreversible damage to the ecological environment

Method used

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  • Streptomyces Phaeochromogenes SICAU417 and application thereof
  • Streptomyces Phaeochromogenes SICAU417 and application thereof
  • Streptomyces Phaeochromogenes SICAU417 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 Isolation of Streptomyces chromogenes SICAU417

[0022] The rhizosphere soil of highland barley in Zhalang County, Tibet was collected by "shaking roots", and after removing debris such as highland barley roots, put it in a sterile plastic bag and seal it and bring it back. Labels were placed inside and outside the bag to indicate the sampling location and sampling date , samplers, etc.

[0023] The dilution plate colony method was used for cultivation, and the collected soil was serially diluted 10 times and inoculated on the improved ISP5 medium plate. After culturing at 28°C for 30 days, a single colony was selected according to the apparent characteristics of the colony size, shape, and color, and purified by streaking, and 34 strains with different colony shapes were obtained by isolation.

Embodiment 2

[0024] Embodiment 2 produces indole acetic acid (IAA) and measures

[0025] The obtained 34 strains of endophytic actinomycetes were cultivated to the logarithmic phase, and 50 μL of the bacterial liquid was taken in the beef extract liquid medium (about 3 mL) containing 0.5 g / L tryptophan, and repeated 3 times, at 28 °C, 140 r / Shake and culture for 1.5 days, take 50 μL on a clean white porcelain plate, add 100 μL of color reagent, keep away from light for 30 minutes at 25°C, if pink color appears, it indicates that IAA is produced. Amino acid beef extract liquid medium was used as a control.

[0026] Continue to culture the endophytic actinomycetes producing indole acetic acid in the beef extract liquid medium containing 0.5g / L tryptophan for 24-48h, centrifuge at 8000r / min for 5min, take 2mL of the supernatant and add 4mL of Salkowski to develop the color After being protected from light for 30 min at 25°C, the absorbance value was measured at 530 nm. Use the blank cultur...

Embodiment 3

[0028] Example 3 Determination of Phosphorus Solubilizing Ability

[0029] The obtained 34 strains were planted on the PVK medium plate, cultured at 30°C for 72 hours, the HD value of the transparent circle diameter and the CD value of the colony diameter were recorded and their ratio was calculated. Among the 34 strains, 8 strains have the ability to dissolve phosphorus, and the HD / CD value of the strain SICAU417 is about 1.65.

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Abstract

The invention discloses Streptomyces Phaeochromogenes SICAU417 and an application thereof. The strain is preserved in the China Center for Type Culture Collection (CCTCC), the address is Luojia hill,Bayi road, Wuchang district, Wuhan city, Hubei province, the preservation date is October 8, 2018, and the preservation number is CCTCC NO:M 2018661. The 16S rRNA sequence of the Streptomyces Phaeochromogenes SICAU417 is shown as in SEQ ID NO.1. The Streptomyces Phaeochromogenes SICAU417 has a wider antibacterial action, promotes the plant growth at the same time, and has the double-effect functions of both a fertilizer and a pesticide, and a microbial fertilizer prepared by using the strain is of great significance for reducing the chemical fertilizer and applying the pesticide in the agricultural planting industry.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to Streptomyces obserochromogenes SICAU417 and its application. Background technique [0002] Highland barley is a crop of the genus Barley in the Gramineae family, a type of naked barley, and is widely planted in Tibet, Sichuan and other places. Low yield and many diseases are the main factors restricting the development of the current highland barley planting industry. Although the application of traditional pesticides and chemical fertilizers can increase the yield of highland barley in a short period of time and alleviate the contradiction between supply and demand, it will cause irreversible damage to the ecological environment. Actinomycetes can decompose a variety of organic matter, regulate soil nutrients, promote the formation of soil aggregate structure to improve soil, and secrete secondary metabolites such as plant growth regulators and disease-resistant ac...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00C05F11/08C05G3/00C05G3/04C12R1/465C05G3/80
CPCA01N63/10C05F11/08C05G3/00C05G3/80C12N1/205C12R2001/465
Inventor 辜运富尼玛扎西刘婷高雪普布贵吉马瑞萍张宗锦赵珂陈强
Owner SICHUAN AGRI UNIV
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