Quick preparation method and application of chickpea polypeptide powder

A technology of chickpea polypeptide and chickpea, which is applied in the field of rapid preparation of chickpea polypeptide powder, can solve problems such as toxic and side effects, and achieve the effect of simple equipment and easy scale-up preparation

Inactive Publication Date: 2016-06-01
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its toxic and side effects, synthetic antioxidants are controlled by the governments of various countries to prevent excessive addition.

Method used

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  • Quick preparation method and application of chickpea polypeptide powder
  • Quick preparation method and application of chickpea polypeptide powder
  • Quick preparation method and application of chickpea polypeptide powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] a. Take 500.00g of chickpea watercress, rinse them with water for 3 times until they are completely clean, filter them with gauze respectively, combine the washed chickpea watercress, dry them at low temperature, and control the temperature at 40°C. Crushed by a pulverizer, the crushed particle size is 200 mesh, and petroleum ether is added according to the weight ratio of material to liquid at 1:5, stirred at room temperature for 1 hour, repeated extraction 3 times until no oil is extracted, suction filtered, combined and precipitated, and naturally Dry until no petroleum ether solvent remains, and obtain 382.19 g of defatted chickpea flour;

[0046] b, adding concentration to the defatted chickpea powder obtained in step a is 50% edible ethanol, the ratio of solid to liquid is 1:5, stirring and extracting at room temperature for 2 hours, extracting 3 times each time, and suction filtration under reduced pressure, Combine the extracts, separate the impurities, and obta...

Embodiment 2

[0051] a. Take 300.00g of chickpea watercress, wash them with water for 3 times until they are completely clean, filter them with gauze respectively, combine the washed chickpea watercress, dry them at low temperature, and control the temperature at 40°C. Crushed by a pulverizer, the crushed particle size is 200 mesh, and petroleum ether is added according to the weight ratio of material to liquid at 1:5, stirred at room temperature for 2 hours, extracted repeatedly for 3 times until no oil is extracted, filtered with suction, combined and precipitated, and naturally Dry until there is no petroleum ether solvent residue to obtain 190.00 g of defatted chickpea flour;

[0052] b. Add edible ethanol with a concentration of 50% to the fully defatted chickpea flour in step a, the ratio of solid to liquid is 1:5, stir and extract at room temperature for 3 hours, extract 3 times each time, filter under reduced pressure, and combine extracting liquid, separating impurities, obtaining ...

Embodiment 3

[0057] a. Take 500.00g of dry organic solvent to extract the chickpea dregs after the small molecular compound, pulverize through a pulverizer, the pulverization particle size is 200 mesh, add petroleum ether according to the weight ratio of material to liquid as 1:5, and stir at room temperature for 1 hour, Repeat the extraction 3 times until no oil is extracted, filter with suction, combine the precipitates, and dry naturally until no petroleum ether solvent remains to obtain 385.00 g of defatted chickpea flour;

[0058] b. Add edible ethanol with a concentration of 50% to the chickpea flour fully defatted in step a, the ratio of solid to liquid is 1:5, stir and extract at room temperature for 2 hours, extract 4 times each time, filter under reduced pressure, and combine extracting liquid, separating impurities, obtaining crude extracting liquid for subsequent use;

[0059] c. Purify the crude extract obtained in step b with a regenerated cellulose flat membrane with a molec...

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Abstract

The invention relates to a quick preparation method and application of chickpea polypeptide powder. The method comprises the following steps: by using chickpea beans or dry chickpea slag obtained by extracting small molecule compounds with an organic solvent as the raw material, pulverizing, degreasing, carrying out a modern ultrafiltration membrane technique, and carrying out modern spray drying or freeze-drying to obtain the chickpea polypeptide powder. The chickpea polypeptide powder proves to have antibacterial and anti-fungus activities, has wide antimicrobial activity, antioxidation activity and blood sugar reduction activity, has the functions of enhancing humoral immunity and general immunity, and contains rich pure natural plant-derived polypeptides and proteins which can be easily digested and absorbed by the human body. The chickpea polypeptide powder maintains the chickpea polypeptide proteins, and achieves the goals of changing wastes into valuable substances and fully utilizing resources. The chickpea polypeptide powder is used as a natural antimicrobial agent, food additive or drug, and can be used in food and medicine industries.

Description

technical field [0001] The invention relates to a rapid preparation method and application of chickpea polypeptide powder, which is a polypeptide part with antibacterial activity prepared by using chickpea watercress and chickpea dregs as raw materials, and has biological activities such as antibacterial and antifungal. It has good application prospects in the fields of medicine and food. Background technique [0002] The discovery of antibiotics was once a milestone in the history of human medicine. However, due to the abuse of antibiotics, the emergence of drug-resistant bacteria occurs from time to time, and the frequency of occurrence exceeds the development speed of new antibiotics, making many highly effective antibiotics invalid, so that many new treatments for infectious diseases have been brought. The use of traditional antibiotics is facing severe challenges. It has become a development trend to research and apply new antibacterial agents that are non-toxic, poll...

Claims

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Application Information

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IPC IPC(8): C07K1/36C07K1/34A61K38/02A61P31/04A61P31/10A23L33/18A23L33/185
CPCC07K1/36A23V2002/00A61K38/02C07K1/145C07K1/34A23V2200/328A23V2200/324A23V2250/55A23V2250/548
Inventor 阿布力米提·伊力阿吉艾克拜尔·艾萨艾合米丁·外力热合满·艾拉
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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