Copper metal organic framework nanoparticles functionalized hydrogel and its preparation method and application

An organic framework and nanoparticle technology, applied in bandages, medical science, etc., can solve the problems of low hydrogel strength and water swelling rate, unsatisfactory wound care, damage to normal cells, etc., and achieve good water retention performance and excellent absorption The effect of wound exudate and intense cell activity

Active Publication Date: 2022-05-24
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Chinese invention patent application CN201610219873.0 discloses a preparation method of a medical temperature-sensitive hydrogel containing nano-silver and its application in the field of wound healing. It is composed of nano-silver and chitosan, but due to the strong cytotoxicity of nano-silver, In the process of sterilization, normal cells will be damaged, and the temperature-sensitive hydrogel has poor drug slow-release performance, which will easily cause the burst release of nano-silver, which is not conducive to wound healing; prior art (Copper-nanoparticle-embedded hydrogel forkilling bacteria and promoting wound healing with photothermal therapy, Tao B, Lin C, Deng Y, et al. Copper-nanoparticle-embedded hydrogel for killing bacteria and promoting wound healing with photothermal therapy [J]. Journal of Materials Chemistry B, 2019, 7.) disclosed a Copper nanoparticles (Cu NPs) chelated with methacrylate-modified gelatin (Gel-MA) and N,N-bis(acryloyl)cystamine (BACA) chelated by free-radical polymerization with photoinitiator to prepare composite water Gel, used in medical dressings to promote the healing of infected wounds, this composite hydrogel showed significant antibacterial effects on both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria, but due to water The gel strength and water swelling rate are low, and it is easily broken after absorbing wound exudate, so it cannot meet the requirements of wound care

Method used

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  • Copper metal organic framework nanoparticles functionalized hydrogel and its preparation method and application
  • Copper metal organic framework nanoparticles functionalized hydrogel and its preparation method and application
  • Copper metal organic framework nanoparticles functionalized hydrogel and its preparation method and application

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Experimental program
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Embodiment 1

[0040] 1) Preparation of glutathione-modified carboxymethyl chitosan (CMCS-GSH): Dissolve 0.5 g of carboxymethyl chitosan ( CMCS) to make a homogeneous solution; 0 g of reduced glutathione (GSH) and 0.0006 g of N-hydroxysuccinimide (NHS) were dissolved in 10 mL of deionized water, uniformly stirred at 250 rpm for 30 min, GSH and NHS were mixed The mixed solution was added to the flask; 0.048g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was dissolved in 10mL of deionized water, and after stirring evenly, the Add into the flask; at room temperature, adjust the reaction mixture in the flask to pH 4.0~5.8 with 0.5mol / mL HCl solution, and react for 6 hours under uniform stirring at 300rpm; then place the product in a dialysis bag (molecular weight cut-off value : 8 ~ 12kD), soaked in deionized water for 3 days (deionized water was changed twice a day) to eliminate unreacted reagents; finally, the mixture solution was freeze-dried at -80 ° C to obtain the de...

Embodiment 2

[0044]1) Preparation of glutathione-modified carboxymethyl chitosan (CMCS-GSH): Dissolve 0.5 g of carboxymethyl chitosan ( CMCS) to make a homogeneous solution; dissolve 0.10 g of reduced glutathione (GSH) and 0.011 g of N-hydroxysuccinimide (NHS) in 10 mL of deionized water, stir evenly for 30 min at 250 rpm, and mix GSH and The mixed solution of NHS was added to the flask; 0.096g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was dissolved in 10mL of deionized water, and after stirring evenly, the It was added to the flask; at room temperature, the reaction mixture in the flask was adjusted to pH 4.0-5.8 with a 0.6 mol / mL HCl solution, and the reaction was uniformly stirred at 300 rpm for 4 hours; then the product was placed in a dialysis bag (molecular weight cut-off). value: 8-12kD), soaked in deionized water for 3 days (changed deionized water twice a day) to eliminate unreacted reagents; finally, the mixture solution was lyophilized at -80 °C to obt...

Embodiment 3

[0047] 1) Preparation of glutathione-modified carboxymethyl chitosan (CMCS-GSH): Dissolve 0.5 g of carboxymethyl chitosan ( CMCS) to make a homogeneous solution; 0.19 g of reduced glutathione (GSH) and 0.011 g of N-hydroxysuccinimide (NHS) were dissolved in 10 mL of deionized water, stirred at 250 rpm for 30 min, and the GSH and The mixed solution of NHS was added to the flask; 0.096g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was dissolved in 10mL of deionized water, and after stirring evenly, the It was added to the flask; at room temperature, the reaction mixture in the flask was adjusted to pH 4.0~5.8 with 0.5mol / mL HCl solution, and the reaction was carried out under uniform stirring at 300rpm for 5 hours; then the product was placed in a dialysis bag (molecular weight cut-off). value: 8-12kD), soaked in deionized water for 3 days (changed deionized water twice a day) to eliminate unreacted reagents; finally, the mixture solution was lyophilized ...

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Abstract

The invention discloses a copper metal organic framework nanoparticle functionalized hydrogel, a preparation method and an application thereof. The preparation method first dissolves carboxymethyl chitosan and then adds reduced glutathione and N-hydroxysuccinimide mixed and dissolved in deionized water; adding 1-(3-dimethylaminopropyl)-3 ‑Ethylcarbodiimide hydrochloride to prepare glutathione-modified carboxymethyl chitosan; copper metal-organic framework nanoparticles; then mix copper metal-organic framework nanoparticles with deionized water; add glutathione Modified carboxymethyl chitosan, acrylamide, polyethylene glycol diacrylate, polyethylene glycol diglycidyl ether and initiator prepared copper metal organic framework functionalized hydrogel; Release copper ions in a controlled manner, remove active oxygen from the wound surface, maintain a 3D microenvironment of the wound surface without accumulation of fluid and moist, protect the wound from external damage, and promote wound healing.

Description

technical field [0001] The invention relates to a functionalized hydrogel, in particular to a copper metal organic framework nanoparticle functionalized hydrogel and a preparation method and application thereof, and belongs to the field of medical devices. Background technique [0002] According to statistics, every year in China, tens of millions of people suffer from skin wounds due to accidents and operations, and hundreds of thousands of people die due to wound infections that fail to heal. Among them, chronic wounds are the more difficult types of wounds to heal. Chronic wound sites produce high concentrations of the by-product H 2 O 2 Reactive oxygen species (ROS) and pro-inflammatory mediators. Excessive production of ROS can lead to oxidative stress, resulting in impaired skin fibroblast and keratinocyte function; a large amount of exudate from chronic wound wounds is easily infected by pathogenic microorganisms. Causes physiological dysfunction of human tissues. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08J3/24A61L26/00C08L5/08C08L87/00C08L71/02C08B37/08C08F283/06C08F220/56C08G83/00
CPCC08J3/075C08J3/246C08J3/243A61L26/008A61L26/0066A61L26/0023A61L26/0019C08B37/003C08F283/065C08G83/008A61L2300/102A61L2300/404C08J2305/08C08J2487/00C08J2471/02C08J2387/00C08J2405/08C08L5/08C08L71/02C08F220/56
Inventor 彭晓宏马晓峰
Owner SOUTH CHINA UNIV OF TECH
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