Microorganism repairing agent for repairing rock cracks and preparation method of microorganism repairing agent

A technology for microbial repair and rock fissures, applied in the field of microbial remediation agents for repairing rock fissures and its preparation, can solve problems such as low volume viscosity and rock fissure leakage, and achieve the effects of low viscosity, high permeability and solving leakage

Inactive Publication Date: 2020-12-25
广西壮族自治区水利科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microbial remediation agent provided by the invention has the characteristics of small size, low viscosity, high permeability, and environmental protection, and can quickly enter narrow rock cracks, and then fill and seal the cracks to solve the problem of rock crack leakage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A microbial remediation agent for repairing rock fissures, its raw materials include bacillus pasteurianus and cementing material; the cementing material includes the following raw materials in parts by weight: urea 26g, calcium chloride 48g, nutrient broth 13g, NaHCO 3 2g and NH 4 Cl 11g; the OD600 value of bacillus pasteurianus in the repair agent is 1.

[0021] The preparation method of described microbial remediation agent, comprises the steps:

[0022] (1) Weigh the gelling liquid material according to the above ratio, add calcium chloride, nutrient broth, NaHCO3 and NH4CL into 1L of water, heat to 80°C to completely dissolve the material, cool to 45°C, add urea, stir evenly, and obtain cementing fluid;

[0023] (2) Dissolve the lyophilized powder of Bacillus pasteurianus in 1 L of deionized water, resuscitate, transfer and expand culture according to the conventional method, measure the bacterial liquid with a visible light spectrophotometer, and prepare a micro...

Embodiment 2

[0026] A microbial remediation agent for repairing rock fissures, its raw materials include bacillus pasteurianus and cementing material; the cementing material includes the following raw materials in parts by weight: urea 21g, calcium chloride 50g, nutrient broth 16g, NaHCO 3 2.5g and NH 4 Cl10g; the OD600 value of bacillus pasteurianus in the repair agent is 0.8.

[0027] The preparation method of described microbial remediation agent, comprises the steps:

[0028] (1) Weigh the gelling liquid material according to the above ratio, add calcium chloride, nutrient broth, NaHCO3 and NH4CL into 1L of water, heat to 80°C to completely dissolve the material, cool to 45°C, add urea, stir evenly, and obtain cementing fluid;

[0029] (2) Dissolve the lyophilized powder of Bacillus pasteurianus in 1L of deionized water, resuscitate, transfer and expand culture according to the conventional method, measure the bacterial liquid with a visible light spectrophotometer, and prepare a mic...

Embodiment 3

[0032] A microbial restoration agent for repairing rock fissures, its raw materials include bacillus pasteurianus and cementing material; the cementing material includes the following raw materials in parts by weight: urea 32g, calcium chloride 53g, nutrient broth 18g, NaHCO 3 2.5g and NH 4 Cl10g; The OD600 value of bacillus pasteurianum in the repair agent is 1.2.

[0033] The preparation method of described microbial remediation agent, comprises the steps:

[0034] (1) Weigh the gelling liquid material according to the above ratio, add calcium chloride, nutrient broth, NaHCO3 and NH4CL into 1L of water, heat to 70°C to completely dissolve the material, cool to 40°C, add urea, stir evenly, and obtain cementing fluid;

[0035] (2) Dissolve the lyophilized powder of Bacillus pasteurianus in 1L of deionized water, resuscitate, transfer and expand culture according to the conventional method, measure the bacterial liquid with a visible light spectrophotometer, and prepare a mic...

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PUM

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Abstract

The invention discloses a microorganism repairing agent for repairing rock cracks and a preparation method of the microorganism repairing agent. The microorganism repairing agent is prepared from thefollowing raw materials of: bacillus pasteurii and a cementing material, the cementing material is prepared from the following raw materials in parts by weight: 17-35 parts of urea, 32-64 parts of calcium chloride, 10-20 parts of nutrient broth, 1.5-2.5 parts of NaHCO3 and 9-12 parts of NH4Cl; the OD600 value of the bacillus pasteurii in the repairing agent is 0.6-1.2. The microbial remediation agent provided by the invention has the advantages of small size, low viscosity, high permeability, environmental friendliness and the like, and can quickly enter narrow rock cracks to fill and seal thecracks so as to solve the problem of rock crack leakage; the product of microbial mineralization is mainly calcite type calcium carbonate; the product has good stability and durability and is good incompatibility with a matrix material; the method that microorganisms are adopted to induce calcium carbonate precipitation is an eco-friendly technology and has the advantage of being green and environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of civil engineering, in particular to a microbial restoration agent for repairing rock fissures and a preparation method thereof. Background technique [0002] In geotechnical engineering, rock cracking and leakage caused by various geological reasons widely exist. These cracks greatly reduce the strength and durability of components. If they are not repaired in time, they will cause huge safety hazards. For example, the distribution of karst landforms in Guangxi accounts for more than 40% of the total area of ​​the autonomous region, among which limestone and dolomitic limestone are the main types. Due to the widespread existence of karst caves and fissures, the long-term erosion and leakage of groundwater has caused serious water and soil separation in karst areas, which has exacerbated the characteristics of karst areas that are prone to drought and waterlogging, as well as landslides and other disasters...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C04B28/00E02D3/00C12N1/20C12R1/07
CPCC04B28/00C12N1/20E02D3/00C04B2103/0001C04B24/00C04B22/002C04B22/10C04B22/124C04B24/126
Inventor 陈建国陈德伟刘鲁强陈春黄旭升郭晋川杜念吴卫熊
Owner 广西壮族自治区水利科学研究院
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