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Method for extracting active polysaccharide of wheat bran

The technology of an active polysaccharide and an extraction method is applied to the processing field of wheat bran, which can solve the problem of low economic value, and achieve the effects of improving product purity, accelerating growth, reproduction and fermentation metabolism, and enhancing condensation reaction.

Inactive Publication Date: 2018-11-06
金华市艾力生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the past, bran was mainly used as feed, with little economic value

Method used

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  • Method for extracting active polysaccharide of wheat bran

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Effect test

Embodiment 1

[0018] The extraction method of wheat bran active polysaccharide specifically comprises the following steps:

[0019] 1) Inoculate the revived Schizophyllum, Bacillus subtilis, lactic acid bacteria and Saccharomyces cerevisiae in a weight ratio of 1:5:1:2 in the nutrient broth medium sterilized at 121°C and 0.1MPa, at 36 Cultivate for 24 hours under the conditions of ℃ and 120r / min. At this time, the bacterial concentration reaches 1.1×10 8 cfu / mL; 2) With 150 parts of bran as the main ingredient, 30 parts of soybean meal powder and 15 parts of corncob powder as auxiliary materials, 1.5 parts of honey and 3 parts of sucrose were added, distilled water was added to reach a water content of 45%, and the 3) Inoculate the mixed seed solution into the fermentation medium at a ratio of 9.8% of the dry weight of the fermentation medium, stir evenly, and place it in a constant temperature incubator at 35°C for 60 hours of fermentation; take the fermented bran Dry the wet sample in an...

Embodiment 2

[0021] The extraction method of wheat bran active polysaccharide specifically comprises the following steps:

[0022] 1) Preparation of seed solution: Inoculate the revived Schizophyllum, Bacillus subtilis, lactic acid bacteria and Saccharomyces cerevisiae in a weight ratio of 1:6:2:3 in the nutrient broth medium sterilized at 121°C and 0.1MPa cultured at 38°C and 150r / min for 26 hours, at this time the cell concentration reached 1.5×10 8 cfu / mL; mixed-bacteria fermentation can play a synergistic effect among various strains, and can reduce the disadvantage of single-bacteria fermentation. There may be symbiosis among the four strains, and the complementary effect of metabolites can overcome the excessive effect of intermediate products on bran. Unfavorable factors for the formation of bran polysaccharides, so it is beneficial to the fermentation of bran polysaccharides;

[0023] 2) Preparation of fermentation medium: 180 parts of bran as the main material, 35 parts of soybea...

Embodiment 3

[0027] A method for extracting active polysaccharides from wheat bran, comprising: preparing seed liquid, preparing a fermentation medium, fermenting, and extracting active polysaccharides, specifically including the following steps:

[0028] Preparation of seed solution: Inoculate the revived Schizophyllum, Bacillus subtilis, lactic acid bacteria and Saccharomyces cerevisiae in a weight ratio of 1:5:1:3 in the nutrient broth medium sterilized at 121°C and 0.1MPa, Cultivate for 24 hours at 37°C and 140r / min, at which point the cell concentration reaches 1.2×10 8 cfu / mL; mixed-bacteria fermentation can play a synergistic effect among various strains, and can reduce the disadvantage of single-bacteria fermentation. There may be symbiosis among the four strains, and the complementary effect of metabolites can overcome the excessive effect of intermediate products on bran. Unfavorable factors for the formation of bran polysaccharides, so it is beneficial to the fermentation of bra...

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Abstract

The invention discloses a method for extracting active polysaccharide of wheat bran. The method comprises seed liquid preparation, fermentation medium preparation, fermentation and active polysaccharide extraction and concretely comprises inoculating nutrient broth with revived schizophyllum commune, bacillus subtilis, lactic acid bacteria and saccharomyces cerevisiae according to a weight ratio,carrying out culture to obtain a seed liquid, mixing wheat bran, soybean meal, corn core powder, distilled water and a fermentation synergist to obtain a fermentation medium, inoculating a fermentation medium with the seed liquid, carrying out fermentation culture, and extracting the fermented wheat bran through distilled water and an ethanol solution to obtain an active polysaccharide solution. The fermentation synergist can greatly increase the yield of the active polysaccharide. The method can significantly reduce the probability that the wheat bran active polysaccharide is separated alongwith the protein denatured gel and increase the yield and content of the wheat bran active polysaccharide. The wheat bran active polysaccharide can be used as an antioxidant active ingredient to remove free radicals in the body.

Description

technical field [0001] The invention relates to the processing field of wheat bran, in particular to a method for extracting wheat bran active polysaccharides. technical background [0002] Wheat bran is a by-product obtained after wheat is processed for flour. In the past, bran was mainly used as feed, and its economic value was not high. In fact, bran can be developed and utilized at multiple levels, with great potential for deep processing and many channels. As a traditional Chinese medicine, it contains a large amount of nutrients necessary for the human body. It has the functions of nourishing the lungs, moisturizing the skin, preventing and fighting cancer, strengthening the spleen and stomach, strengthening the hair, cleaning the stomach, etc., and has high medical and health care value. According to the determination of modern scientific research, the content of p-aminobenzoic acid is the highest among plants. p-aminobenzoic acid is an essential substance for human...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P19/04A23L33/125C12R1/125C12R1/865C12R1/645
CPCC12P39/00A23L33/125A23V2002/00C12P19/04A23V2200/30A23V2300/14
Inventor 周艳初
Owner 金华市艾力生物科技有限公司
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