64 results about "Lactobacillus fornicalis" patented technology

The invention discloses microbial feed additive used for pigs, and a preparation process thereof; the prescription is as follows: 1 portion of bacillus licheniformis, 1 portion of bacillus subtilis, 2 portions of bifidobacterium, 2 portions of enterococcus faecalis, 2 portions of enterococcus faecium, 1 portion of boas-oppler bacillus, 1 portion of lactobacillus casei, 1 portion of lactobacillus lactis, 1 portion of plant lactobacillus, 1 portion of candida utilis, 1 portion of saccharomyces cerevisiae, 80kg to 100kg of panela, 3kg to 5kg of salt, 5kg to 8kg of vinegar, 3kg to 5kg of lysine, 1kg to 2kg of methionine, and 1000L of pure water; the weight of each portion of strain is 2g to 5g. The preparation process is as follows: strain preparation, nutrient broth preparation, inoculated fermentation and check. The invention reduces the quantity of the used individual strain, and the strain quality is safe and reliable. The production process is simple and feasible, the effect is obvious; therefore, the invention has promotion value.

The present invention relates a novel Lactobacillus sp. Strain and usc thereof. More specifically, the present invention relates to a novel Lactobacillus sp. Strain, Lactobacillus paracasei, isolated and identified from Kimchi fermentation liquid and uses thereof as probiotics, feeding additive, deodorizing agent and food additive. Lactobacillus paracasei of the present invention has good acid-resistance, bile acid-resistance and stability in the human body, has pathogenic bacteria inhibiting activity, increases numbers of beneficial bacteria by changing intestinal microflora and decreases diarrhea incidence, deodorizes offensive odor, and has effectes of softening and deodorizing in meat and fish.

Processes to produce microorganisms that can be incorporated into a microbial-based product that results in high viable cell yields and shelf-stable products are disclosed. These microbial-based products are useful for inhibiting pathogenic growth and as a food additive. A preferred microorganism is the lactic acid producing bacteria, Lactobacillus amylovorus M35. In one embodiment, the process comprises inoculating a lactobacillus fermentation medium with M35 cells, harvesting the M35 cells at mid to late log phase, concentrating the M35 cells, and preserving the M35 cells at a concentration of at least 5×109 cfu / ml.

The invention provides a pig feed for improving growth performance and immunization function of weanling pig, which is characterized by comprising a feed carrier, nicotinic acid, calcium pantothenate, biotin, vitamin C, Lactobacillus lactis, lactobacillus plantarum, amylase, protease, fish oil, zein powder, grease, methionine, tryptophan, threonine, lysine, ethoxy quinoline and a traditional Chinese medicine additive. The traditional Chinese medicine composition is added to the feed to improve the growth performance and immunization function of the weanling pig, thereby promoting the healthy growth of the baby pig.

The invention relates to the use of at least two lactic acid bacterial strains selected from the group comprising Pediococcus pentosaceus 16:1 (LMG P-20608), Leuconostoc mesenteroides 23-77:1 (LMG P-20607), Lactobacillus paracasei subsp paracasei F-19 (LMG P-17806), and Lactobacillus plantarum 2362 (LMG P-20606) for the manufacturing of a formulation for the prevention or treatment of stress-induced inflammatory disorder.

The invention discloses a method for preparing complete feed through probiotics asynchronous high-low-moisture fermentation and belongs to the technical field of microbial feed. The method comprises the following concrete steps that: firstly, protein feed is subjected to impurity removal and crushing, nutrition substances are added, mixed and stirred with clean water, and the moisture content is regulated to 60 to 70 percent; then, yeast, bacillus licheniformis and bacillus subtilis strain liquid is inoculated, high-moisture-content aerobic fermentation is carried out for 12 to 96 hours, and the fermentation temperature is 35 to 45 DEG C; then, energy feed is added, mixing and stirring are carried out, and the moisture content is regulated to 25 to 35 percent; next, lactobacillus lactis, enterococcus faecium, lactobacillus acidophilus, enterococcus faecalis and lactobacillus plantarum strain liquid is added, complex enzyme is added, the low-moisture-content anaerobic fermentation is carried out for 24 to 120 hours, and the fermentation temperature is 25 to 35 DEG C; and finally, the complete feed finished product is prepared through rolling tube drying, mixing and stirring, granulation and package. The method has the beneficial effect that the production efficiency and the feed nutrition value are improved.

The invention relates to lactococcus lactis subsp.lactis, antibacterial peptide produced by lactococcus lactis subsp.lactis and the application of antibacterial peptide. In the invention, the collection number of the lactococcus lactis subsp.lactis LLC518 in the General Microorganism Center of China Microorganism Culture Collection Management Committee is CGMCC No. 4584. In the invention, the antibacterial peptide produced by the lactococcus lactis subsp.lactis can restrain micrococcus tetragenus, Bacillus thuringiensis, lactococcus lactis and listeria innocua, particularly, has strong restraint function to L. monocytogenes which can cause serious food poisoning. Thus, the lactococcus lactis subsp.lactis and the antibacterial peptide thereof have active function in food fresh keeping.

The present invention relates to one kind of micro ecological feed additive capable of promoting pig's growth and its preparation process. Technologically, the present invention features that Bacillus subtilis, Bacillus licheniformis, saccharomycete, lactobacillus and Bacillus lacticus as well as carrier in certain proportion as materials are mixed homogeneously to obtain the micro ecological feed additive capable of promoting pig's growth. The present invention has the functions of inhibiting the growth of pathogenic bacteria, stimulating the immune reaction of pig, strengthening the stress resisting capacity of pig, providing pig with nutrients, etc. and obvious effect of promoting the growth of pig. The spawn producing process has advanced liquid fermentation and spray drying technology adopted and results in high purity and activity of the prepared live microbial preparation.

The invention provides a granulation-resisting lactic acid bacterium preparation and a preparation method thereof. The granulation-resisting lactic acid bacterium preparation comprises lactic acid bacterium; the lactic acid bacterium is one or a combination of more of enterococcus faecalis, enterococcus faecium, lactobacillus plantarum, lactobacillus acidophilus, lactobacillus lactis, lactobacillus buchneri, lactobacillus casei and bifidobacterium animalis; a viable count of the lactic acid bacterium reaches 1.0*10<10> to 1.0*10<11>CFU / g. According to the preparation method of the lactic acidbacterium preparation, provided by the invention, the tolerance of thalli is improved and the loss of bacterium activity in a processing process is avoided.

The invention involves a microbial feed additive and its prepare methods which improveboar fecundity, it is characterized by:lactic acid lactobacillus and bacillus subtilis which are optimized make the products rich in beneficial microbios and variety of active metabolite through the means of combining pedigree breed fermentation and composite fermentation. The advantages of the invention are maintaining boar body health,repairing tissue injury, improving the fecundity and the body immune function, thus making the boar maintain strong sexuality and produce high grade semen, extending boar life, and nontoxic, nonpersistent and the use is convenience.

The invention relates to an intestine-regulating constipation-removing enzyme and a preparation method thereof. The enzyme comprises the following raw materials in percentage by weight: 5% of figs, 10% of sophora flowers, 15% of celery, 20% of pears, 20% of apples, 2% of radix paeoniae alba, 4% of plantain, 3% of liquorice, 20% of mulberries, 2% of dandelion, 20% of flaccid knotweed herb and 80% of coarse rice. The preparation method comprises the following steps: 1. steaming the coarse rice in the raw materials, alcoholizing the steamed coarse rice and the flaccid knotweed by hansenula anomala and saccharomyces cerevisiae, and after alcoholizing, adding water, acetifying by acetobacter pasteurianus and clostridium butyricum; 2. grinding the other materials, alcoholizing by honey yeasts, hansenula anomala, rhodotorula glutinis, saccharomyces cerevisiae and streptomyces microflavus, after finishing the steps 1 and 2, mixing according to a ratio of 1 to 1, lactifying by lactobacillus lactis and plant lactobacilli, and fermenting to obtain suspension liquid as the intestine-regulating constipation-removing enzyme. The intestine-regulating constipation-removing enzyme has the effects of promoting production of body fluid, moistening the lung and facilitating intestinal tract movement, and is suitable for crowds with constipation.

The invention discloses a direct vat set starter of preserved szechuan pickle and preparation method thereof. The direct vat set starter of preserved szechuan pickle is inoculated for MRS cultivation by using leuconostoc mesenteroides, lactobacillus plantarum and lactobacillus lactis from brine of preserved szechuan pickle; amplified cultivation is conducted; leuconostoc mesenteroides powder, lactobacillus plantarum powder and lactobacillus lactis powder are respectively prepared after centrifuging, freezing and drying; and the three types of bacterium powders are proportioned into compound lactobacillus powder at the weight ratio of 60-70: 15-20: 15-20, and then the direct vat set starter of preserved szechuan pickle is obtained. The direct vat set starter of preserved szechuan pickle in the invention is easy to store and preserve, convenient to carry and use, and simple and quick to prepare. When being used for fermenting preserved szechuan pickle, the product of the invention not only can reserve original taste and quality of the preserved szechuan pickle that is fermented by the traditional method, but also can effectively reduce the content of nitrite in the product and obviously shorten the fermentation cycle of the preserved szechuan pickle, thereby improving the use safety of the product and reducing the production cost of the preserved szechuan pickle.

A composition comprising lactic acid bacteria that are selected from at least one of Lactococcus lactis, Lactobacillus lactis, and Lactobacillus brevis. The composition is suitable for administration to animals. Upon administration to an animal, the composition provides at least one of the following (a) improves performance in the animal and (b) reduces scours in animals. Also provided is a method of treating an animal in which the composition is administered to the animal. A method of forming a direct-fed microbial is also provided. A culture including the lactic acid bacteria is grown in a liquid nutrient broth. The lactic acid bacteria are separated from the liquid nutrient broth. The lactic acid bacteria can be freeze dried.

The present invention relates a novel Lactobacillus sp. strain and use thereof. More specifically, the present invention relates to a novel Lactobacillus sp. strain, Lactobacillus paracasei, isolated and identified from Kimchi fermentation liquid and uses thereof as probiotics, feeding additive, deodorizing agent and food additive. Lactobacillus paracasei of the present invention has good acid-resistance, bile acid-resistance and stability in the human body, has pathogenic bacteria inhibiting activity, increases numbers of beneficial bacteria by changing intestinal microflora and decreases diarrhea incidence, deodorizes offensive odor, and has effectes of softening and deodorizing in meat and fish.

The invention relates to a fodder addictive for the promotion of animal fur growth, which comprises probiotic composition and carrier, the carrier being the mixture of zeolite powder and oligosaccharide, the probiotic composition comprises (by weight ratio) bacillus subtilis 1-8 parts, bacillus licheniformis 1-6 parts, yeast 1-6 parts, lactic acid bacteria 1-8 parts, Lactobacillus acidophilus 1-6 parts. The weight ratio of the probiotic composition and carrier is 0.5-2:1.

The invention relates to a feed additive, and particularly relates to a microbial fermentation additive and a preparation method as well as application thereof. The preparation method of the microbial fermentation additive comprises the following steps of mixing bacillus licheniformis, bacillus subtilis, bifidobacterium bifidum, enterococcus faecalis, lactobacillus acidophilus, lactobacillus casei, lactobacillus lactis, lactobacillus plantarum, pediococcus acidilactici, pediococcus pentosaceus, candida utilis, saccharomyces cerevisiae and rhodopseudomonas palustris microbial inoculum according to a proportion by a viable count per unit mass, which is (500 to 600): (500 to 600): (10,000 to 75,000): (10,000 to 73,000): (10,000 to 75,000): (10,000 to 73,000): (10,000 to 73,000): (10,000 to 65,000): (10,000 to 60,000): (10,000 to 75,000): (10,000 to 75,000): (50 to 70): 1, and obtaining the microbial fermentation additive through preparation. According to the microbial fermentation additive and the preparation method as well as the application thereof, straw is subjected to fermentation treatment through a synergistic effect among strains; the fermentation level of the straw can be quickly improved; the deficiency that a single strain secretes enzymes and synthetics is made up; the fermentation quality is improved; meanwhile, by generating many kinds of organic acid and mycoprotein, the palatability of the straw can be improved; the feed intakes of livestock and poultry are improved; the production properties of the livestock and the poultry are obviously improved.

The invention relates to lactobacillus for inhibiting pathogenic bacteria of vaginitis, wherein the lactobacillus is deposited in the China General Microbiological Culture Collection Center and is selected from a group consisting of Lactobacillus acidophilus with the preserving number CGMCC No.6244, Lactobacillus delbrueckii subsp.Lactis with the preserving number CGMCC No.6245 and Lactobacillus plantarum with the preserving number CGMCC No.6246. The lactobacillus provided by the invention can be attached to vaginal cells of a host, stays in the host body for a long time to generate a good bacteriostatic ability, and can secrete massive hydrogen peroxide to generate oxides which are toxic to vaginal pathogenic bacteria, so that the lactobacillus has excellent bacteriostatic effect to pathogenic bacteria of mycotic vaginitis and pathogenic bacteria of bacterial vaginitis, and can be widely applied to preparing commercial products for treating or preventing infection of pathogenic bacteria of vaginitis, such as health-care products and medical preparations. The lactobacillus provided by the invention can be independently used or mixed with other required components to process and prepare products in various forms so as to effectively control infection of pathogenic bacteria of vaginitis and related diseases caused by pathogenic bacteria of vaginitis.

The invention relates to a composite fungicide for treating sludge and a sludge treatment method. The composite fungicide is prepared from the following components in parts by weight: 3 to 8 parts of purple sulfur bacteria, 3 to 8 parts of green sulfur bacteria, 3 to 8 parts of rhodopseudomonas, 3 to 8 parts of rhodopseudomonas capsulata, 3 to 8 parts of rhodococcus rhodochrous, 3 to 8 parts of white-rot fungi, 3 to 8 parts of phosphorus-accumulating bacteria, 3 to 8 parts of anaerobic ammonium oxidation bacteria, 3 to 8 parts of lactobacillus plantarum, 3 to 8 parts of streptomycete, 3 to 8 parts of candida utilis, as well as saccharomyces cerevisiae, lactobacillus lactis, lactobacillus acidophilus, aspergillus niger, aspergillus oryzae, bacillus subtilis and bacillus licheniformis.

The present invention relates to a probiotic anti-oxidation health vinegar production method. According to the method, a variety of probiotics are used for brewed edible vinegar fermentation; at an alcoholic fermentation stage, probiotics of black mold, Aspergillus oryzae and anaerobic Bacteroidaceae are added, wherein the probiotics of the black mold, the Aspergillus oryzae and the anaerobic Bacteroidaceae can be adapted to amylase growth and yeast growth after being cultured; at an acetic acid fermentation stage, probiotics of Lactobacillus fermentum, Lactobacillus lactis, lactobacillus plantarum, Pediococcus acidilactici and Bifidobacterium adolescentis are added to perform synergetic fermentation, wherein the probiotics of the Lactobacillus fermentum, the Lactobacillus lactis, the lactobacillus plantarum, the Pediococcus acidilactici and the Bifidobacterium adolescentis can be adapted to acetic acid bacterial growth within a temperature of 38-46 DEG C after being cultured; and the probiotic liquid vinegar completing the brewed edible vinegar fermentation is sterilized, and a proper amount of white granulated sugar is added to prepare the brewed edible vinegar having the anti-oxidation substance, wherein the vinegar lees can further be baked into the filler of the pig feed and the animal company feed. With the production method of the present invention, the application ranges of the selected culture medium materials of black mold, Aspergillus oryzae, anaerobic Bacteroidaceae, Lactobacillus fermentum, Lactobacillus lactis, lactobacillus plantarum, Pediococcus acidilactici, Bifidobacterium adolescentis and other probiotics are substantially broadened, and the novel brewed edible vinegar inactivated and fermented through the probiotics is provided for consumers.

It used as carrier of bioactive substances of natural origin like dry herb extracts or combinations thereof, other vegetable products as essential oils, fruit concentrates, animal products as bee products, added to vitamins combinations, said product ensuring bacteria proliferation and growth when stored and after intake. The bioactive products can be stored for period 2 to 3 years without the probiotic losing its activity. In the gastroinitestinal tract they can colonize supplying the body with useful substances resulting from their metabolism ensuring fast transportation of bioactive substances to body's cells. It contains low-lactose sour-milk product, low-lactose hydrolyzate with more than 95% degraded milk casein, saccharose, pectin, full-cream or skimmed low-lactose cow's milk fermented using selected strains of Lactobacillus delbrueckii subsp. bulgaricus, and additionally lyophilized monoculture of Lactobacillus delbrueckii subsp. bulgaricus or lyophilized mixture of monocultures of Lactobacillus bulgaricus, Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus lactis and dextrose.

The invention provides a composite biological deodorant. The composite biological deodorant comprises aerobic composite bacteria and anaerobic composite bacteria, wherein the aerobic composite bacteria is formed by performing cofermentation on bacillus subtilis, saccharomyces cerevisiae, rhodopseudomonas palustris, nitrifying bacteria and nitrogen-fixing bacteria in an aerobic condition; the anaerobic composite bacteria is formed by performing cofermentation on lactobacillus lactis and lactobacillus plantarum. The composite biological deodorant can remove various malodorous gases produced by alarge amount of livestock manure produced during breeding, ensures that the removal efficiency of ammonia and sulfuretted hydrogen in henhouses reaches 95 percent or higher and the removal efficiencyof ammonia and sulfuretted hydrogen in manure storage tanks of hog houses reaches 85 percent or higher, is high in effect perdurability and beneficial to improvement of breeding environment, reducesambient environment pollution, and is safe and nonhazardous.

The invention relates to a method for pickling sour and sweet leaf mustards. The method comprises the following steps of: scraping to remove peel after cleaning heads of leaf mustards, cutting into long strips, putting in boiling water to immerse, putting in specially blended green plum liquid to immerse, inoculating lactobacillus lactis bacterium solution, fermenting at 35-38 DEG C for 3-4 days, sterilizing at 80-85 DEG C, continuously putting the long strip-shaped leaf mustards in the green plum liquid to immerse for 10-12 days, taking out the long strip-shaped leaf mustards, and sterilizing at high temperature. The apricot kernel leaf mustards prepared by the invention are sour, sweet, delicious, full-bodied in fragrance and abundant in nutrition and have the effects of tonifying liver, nourishing stomach and promoting the production of body fluid to relieve thirst; no any preservative and colour enhancing agent are added in the pickling process; the pickling antibacterial effect is higher; the pickling antibacterial effect can be kept for a long time without addition of a preservative; acid-base balance of human bodies can be adjusted; secretion and activation of human hormone are promoted; the effects of reducing fat and grease are obvious; and therefore, if being eaten frequently, the sour and sweet leaf mustards have excellent conditioning effects to human bodies.

The invention discloses a silkworm excrement particle-containing composite feed and a preparation method thereof, the composite feed mainly comprises the following ingredients by weight: 40-70 parts of spinach beet, 20-30 parts of nettle, 20-30 parts of corn flour, 15-25 parts of bean cake powder, 20-30 parts of cavings and 15-25 parts of silkworm excrement nutrition additive. The silkworm excrement nutrition additive mainly comprises the following ingredients by weight: 60-85 parts of silkworm excrement, 5-10 parts of silkworm chrysalis, 5-10 parts of ginger powder, 5-10 parts of fish meal, 1-5 parts of methionine, 1-5 parts of lysine and 1-5 parts of probiotics dry powder. The probiotics dry powder is composed of the probiotics with the following weight ratio: lactobacillus lactis to bifidobacteria to bacillus licheniformis is 4: 3: 2 The silkworm excrement particle-containing composite feed has the efficacy of comprehensive nutrition, low cost and daily health care.

The present invention discloses a production method of new functional vinegar drink by probiotic fermentation. Cultured growing probiotics of aspergillus niger, aspergillus oryzae and anaerobic bacteroides adapting to amylase and yeast are added in an alcoholic fermentation stage; and cultured growing lactobacillus fermentum, lactobacillus lactis, lactobacillus plantarum, pediococcus acidilactici, and bifidobacterium adolescentis adapting to acetic acid bacteria in an acetic acid fermentation stage, then the completed probiotic liquid vinegar is sterilized, a right amount of white granulated sugar is added, and the mixture is prepared into fermented edible vinegar with antioxidants. The production method greatly broadens the application scopes of material selections of probiotic cultures of the aspergillus niger, aspergillus oryzae, anaerobic bacteroides, lactobacillus fermentum, lactobacillus lactis, lactobacillus plantarum, pediococcus acidilactici, bifidobacterium adolescentis and other probiotics, and provides consumers with the probiotics enzyme deactivated fermented new functional vinegar drink. The new functional vinegar drink is high in nutrients, rich in sources of raw materials, low in cost, stable in production technology, less in production device investment, and simple in operation and easy to promote.

The invention relates to a method for fermenting and producing fermented vinegar by using various probiotics. The method is characterized in that in an alcohol fermentation stage, cultured probiotics, which are suitable for the growth of amylase and yeast, of aspergillus niger, aspergillus oryzae and anaerobic bacteroides are added; in an acetic fermentation stage, cultured probiotics, which are suitable for the growth of acetic acid bacteria under 38-46 DEG C, of fermentation lactobacillus, lactobacillus lactic, lactobacillus plantarum, pediococcus acidilactici and bifidobacterium adolescentis are added for synergic fermentation, probiotics liquid vinegar obtained after the fermentation of the fermented vinegar is sterilized, and an appropriate amount of white granulated sugar is added to obtain the fermented vinegar with antioxidant substances. Vinegar residues can be dried to produce the filling materials of pig feed and animal feed. The method has the advantages that the material selection application range of probiotics culture media such as the aspergillus niger, aspergillus oryzae, anaerobic bacteroides, fermentation lactobacillus, lactobacillus lactic, lactobacillus plantarum, pediococcus acidilactici and bifidobacterium adolescentis is greatly widened, the novel fermented vinegar inactivated and fermented by the probiotics is provided for consumers, and the method is rich in raw material source, low in cost, stable in production process, low in production equipment investment, simple to operate, easy to popularize and promising in market application and development prospect.

The invention discloses a feed for laying hens and a preparation method thereof. The feed for laying hens is composed of the following raw materials in parts by weight: 50-65 parts of corn, 15-20 parts of soybean meal, 3-5 parts of corn gluten meal, 4-6 parts of corn germ cakes, 2-5 parts of rice bran, 8-10 parts of stone powder, 1-2 parts of soybean oil, 0.1-0.3 part of compound trace element, 0.2-0.5 part of compound multi-vitamin, 0.6-0.9 part of sodium bicarbonate, 0.1-0.3 part of isomalto-oligosaccharide, 0.2-0.4 part of saccharomyces cerevisiae culture, 0.1-0.2 part of coated organic acid, and 0.05-0.1 part of lactobacillus lactis. Through reasonable feed nutrient regulation and compounding of functional additives, the feed can improve the health and disease resistance of laying hens, reduce the number of salmonella in the intestines of the laying hens, improve intestinal health and improve production performance.

The invention provides a lactobacillus lactis MD-622 and an application thereof. The lactobacillus lactis MD-622 can promote the production of protease, amylase and cellulase, and has the potential capability of promoting the digestion of human intestinal tracts. In addition, the lactobacillus lactis MD-622 disclosed by the invention can also promote the growth and development of embryonic stem cells to a certain extent.