Microbial fermentation additive and preparation method as well as application thereof

An additive and micro-storage technology, applied in the direction of bacteria used in food preparation, application, food processing, etc., can solve the problems of long fermentation time, unfavorable straw feed preparation, single bacteria secreting enzymes, and insufficient synthetic substances. It achieves the effects of making up for the deficiency of secreted enzymes and synthetic substances, having a rich and mellow smell, and a simple and practical preparation process

Inactive Publication Date: 2016-06-22
HEILONGJIANG HEIZUAN JIGUANG NEW ENERGY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] About 600 million tons of straw are produced in my country every year, of which about 70% are consumed in the form of living energy, less than 8% are fed to livestock after treatment, and the other part is returned to the field in the form of fertilizer, causing env

Method used

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  • Microbial fermentation additive and preparation method as well as application thereof
  • Microbial fermentation additive and preparation method as well as application thereof
  • Microbial fermentation additive and preparation method as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The preparation of the straw micro-storage additive comprises the following steps:

[0060] (1) Activation of bacteria

[0061] The preserved Bacillus licheniformis, Bacillus subtilis, Bifidobacterium bifidum, Enterococcus lactis, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus lactis, Lactobacillus plantarum, Pediococcus lactis, Pediococcus pentosaceae, pseudococcus utilis Trichosaccharomyces, Saccharomyces cerevisiae and Rhodopseudomonas palustris were placed in sterilized 1% sugar water and activated for 2 hours, and then the spores were transferred into the sterilized common broth medium, the lactic acid bacteria were transferred into MRS, and the yeasts were transferred into Put it into YPD slant medium, place it at 28-35°C, put it into sterilized common broth MRS and YPD medium after the colony grows, and culture it in the shaker at the same temperature as the corresponding slant medium for 24 -48h;

[0062] (2) Preparation of seed solution

[006...

Embodiment 2

[0086] The preparation of the straw micro-storage additive comprises the following steps:

[0087] (1) Activation of bacteria;

[0088] (2) Seed solution preparation;

[0089] (3) Medium preparation and inoculation;

[0090] (4) fermentation;

[0091] (5) Bacteria collection and bacterial agent preparation; wherein, steps (1)-(5) are the same as in Example 1;

[0092] (6) The preparation of micro-storage additive comprises the following steps:

[0093] (a) Determination of the number of live bacteria per unit mass of the freeze-dried agent: Take out several grams of the preserved freeze-dried agent, quickly add 4°C pre-cooled sterile distilled water and shake at 22°C for 10 minutes to resuspend the bacteria, and count the live bacteria after doubling dilution , determine the formula for calculating the number of live bacteria in the freeze-dried agent:

[0094] W=m / (n×V 0 / V 1 )

[0095] W = number of live bacteria per unit mass, cfu / g;

[0096] m = sample mass, g;

...

Embodiment 3

[0107] The application of the prepared micro-storage additive:

[0108] (1) Prepare bacterial solution

[0109] Take 3-5 grams of live dried bacteria and activate them in 500 mL of 1% brown sugar water for 1-2 hours, add 0.9% normal saline to the revived bacteria liquid, make up to 1000 L, mix evenly, and set aside. The bacterial solution is used up on the same day and cannot be used overnight. See Table 3 for the amount of bacterial solution and treated straw.

[0110] Table 3 Bacterial Solution Mixing Ratio

[0111] straw

Straw weight (kg)

Bacteria dosage (g)

Salt consumption (kg)

Tap water consumption (L)

yellow corn stalks

1000

4

8

800

[0112] (2) Collection and pretreatment of straw

[0113] Collect mature yellow corn stalks, discard mildew and severe insect damage, and remove silt, stones, metal and other particles after air drying; cut them into 5-8cm long pieces with a kneading machine for later use.

[0114]...

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Abstract

The invention relates to a feed additive, and particularly relates to a microbial fermentation additive and a preparation method as well as application thereof. The preparation method of the microbial fermentation additive comprises the following steps of mixing bacillus licheniformis, bacillus subtilis, bifidobacterium bifidum, enterococcus faecalis, lactobacillus acidophilus, lactobacillus casei, lactobacillus lactis, lactobacillus plantarum, pediococcus acidilactici, pediococcus pentosaceus, candida utilis, saccharomyces cerevisiae and rhodopseudomonas palustris microbial inoculum according to a proportion by a viable count per unit mass, which is (500 to 600): (500 to 600): (10,000 to 75,000): (10,000 to 73,000): (10,000 to 75,000): (10,000 to 73,000): (10,000 to 73,000): (10,000 to 65,000): (10,000 to 60,000): (10,000 to 75,000): (10,000 to 75,000): (50 to 70): 1, and obtaining the microbial fermentation additive through preparation. According to the microbial fermentation additive and the preparation method as well as the application thereof, straw is subjected to fermentation treatment through a synergistic effect among strains; the fermentation level of the straw can be quickly improved; the deficiency that a single strain secretes enzymes and synthetics is made up; the fermentation quality is improved; meanwhile, by generating many kinds of organic acid and mycoprotein, the palatability of the straw can be improved; the feed intakes of livestock and poultry are improved; the production properties of the livestock and the poultry are obviously improved.

Description

technical field [0001] The invention relates to the field of feed, and mainly relates to a feed additive, in particular to a micro-storage additive, its preparation method and application. Background technique [0002] Straw micro-storage feed is a new straw processing technology that has developed rapidly in recent years. It uses microorganisms to ferment in straw to make straw into roughage with sour, fragrant and wine flavors. It is an energy-saving, environmentally friendly and efficient agricultural product. Farm products. Micro-storage feed has been widely used because of its low cost, high benefit, good palatability, high feed intake, wide source of feed, and not subject to seasonal restrictions. [0003] About 600 million tons of straw are produced in my country every year, of which about 70% are consumed in the form of living energy, less than 8% are fed to livestock after treatment, and the other part is returned to the field in the form of fertilizer, causing env...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/30
CPCA23V2400/125A23V2400/113A23V2400/517A23V2400/169Y02P60/87
Inventor 曾蜀斌
Owner HEILONGJIANG HEIZUAN JIGUANG NEW ENERGY TECH CO LTD
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