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Granulation-resisting lactic acid bacterium preparation and preparation method thereof

A technology of lactic acid bacteria and lactobacillus, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of low granulation survival rate, poor heat resistance and stability of lactic acid bacteria, etc., to avoid the loss of bacterial activity, Effect of improving temperature resistance and improving tolerance

Active Publication Date: 2018-03-20
BEIJING HESWOF BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In view of this, the object of the present invention is to provide a kind of high-efficiency lactic acid bacteria preparation and preparation method thereof, to solve the problems such as poor thermal stability of lactic acid bacteria, low granulation survival rate, etc.

Method used

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  • Granulation-resisting lactic acid bacterium preparation and preparation method thereof
  • Granulation-resisting lactic acid bacterium preparation and preparation method thereof
  • Granulation-resisting lactic acid bacterium preparation and preparation method thereof

Examples

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Effect test

Embodiment 1

[0036] The preparation of embodiment 1 lactic acid bacteria preparation

[0037] (1) Activation of bacteria

[0038] After thawing the various lactic acid bacteria (Enterococcus faecalis, Enterococcus faecium, Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus lactis, Lactobacillus brucei, Lactobacillus casei, Bifidobacterium animalis) preserved in glycerin tubes, in sterile conditions Next, separate by streaking on the MRS plate, culture at 35°C for 48h, pick a single colony from the MRS plate, streak on the slant, culture at 35°C for 16h, and store for future use.

[0039] (2) Level 1 training

[0040] Take the vigorously growing slant respectively, and then use a sterilized inoculation loop to scrape 1 ring of bacteria into 100 mL of primary culture medium, culture at 35°C for 10-20 hours, and obtain the primary culture solution of each lactic acid bacteria.

[0041] (3) Secondary training

[0042] Inoculate the primary culture solution of each lactic acid...

Embodiment 2

[0056] Embodiment 2 Lactic acid bacteria preparations are resistant to artificial gastric juice and artificial intestinal juice determination

[0057] The time for food to stay in the animal stomach is generally 1-2 hours. In this test, the lactic acid bacteria preparation was placed in the simulated artificial gastric juice (pH1.5, pH2.5, pH3.5, pH4.5) for 3 hours, every hour Detect once activity, the influence of artificial gastric juice on the activity of lactic acid bacteria preparation lactic acid bacteria is shown in Table 1. As can be seen from Table 1, when the pH of the artificial gastric juice is 4.5, the survival rate of lactic acid bacteria reaches 95.05% after 3 hours of treatment; when the pH of the artificial gastric juice is 3.5, the survival rate of lactic acid bacteria reaches 93.75% after 3 hours of treatment; When pH is 2.5, the survival rate of lactic acid bacteria reaches 91.11% after 3 hours of treatment; when the pH of artificial gastric juice is 1.5, t...

Embodiment 3

[0063] Embodiment 3 lactic acid bacteria preparation bile salt resistance assay

[0064] The lactic acid bacteria preparation was placed in different concentrations of bile salt solutions (0.03%, 0.15%, 0.3%, 0.5%) to process for 3h, and the activity was detected every hour. As can be seen from Table 3, when the bile salt concentration is 0.03%, the survival rate of lactic acid bacteria reaches 100% for 3 hours; when the bile salt concentration is 0.15%, the survival rate of lactic acid bacteria reaches 99.5% for 3 hours; %, after treatment for 3 hours, the survival rate of lactic acid bacteria reached 95.26%; when the bile salt concentration was 0.5%, after treatment for 3 hours, the survival rate of lactic acid bacteria reached 93.14%.

[0065] The impact of table 3 bile salt on the activity of lactic acid bacteria preparation lactic acid bacteria

[0066]

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Abstract

The invention provides a granulation-resisting lactic acid bacterium preparation and a preparation method thereof. The granulation-resisting lactic acid bacterium preparation comprises lactic acid bacterium; the lactic acid bacterium is one or a combination of more of enterococcus faecalis, enterococcus faecium, lactobacillus plantarum, lactobacillus acidophilus, lactobacillus lactis, lactobacillus buchneri, lactobacillus casei and bifidobacterium animalis; a viable count of the lactic acid bacterium reaches 1.0*10<10> to 1.0*10<11>CFU / g. According to the preparation method of the lactic acidbacterium preparation, provided by the invention, the tolerance of thalli is improved and the loss of bacterium activity in a processing process is avoided.

Description

technical field [0001] The invention relates to a probiotic agent, in particular to a granulation-resistant lactic acid bacteria preparation and a preparation method thereof. Background technique [0002] Lactic acid bacteria preparation is a very effective live bacteria preparation for livestock and poultry breeding, which can improve the digestion and health of livestock and poultry. But lactic acid bacteria are generally sensitive to heat and oxygen. The culture temperature of lactic acid bacteria is generally 35°C to 40°C. In a high temperature environment above 40°C, many lactic acid bacteria will die. Therefore, be in the temperature of the solid medium of the high temperature of state such as steam sterilization, can not add lactic acid bacteria when cooling down to less than 40 ℃, and because the cooling of solid medium needs to spend certain time (according to the different cooling of the amount of solid medium The time required is also different), so it takes a l...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18C12R1/46C12R1/25C12R1/23C12R1/225C12R1/245C12R1/01
CPCA23K10/18C12N1/20
Inventor 李雪平刘建芳尹望杜志琳易总李爱平袁武
Owner BEIJING HESWOF BIOTECH CO LTD
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