Preparation method of load type biological iron-manganese composite oxide

A composite oxide, biological iron technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of water environment quality impact and high water solubility, and achieve low treatment costs, simple operation, and unique The effect of secondary pollution

Inactive Publication Date: 2017-11-24
QINGDAO HAICHENG INTPROP SERVICES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phenylarsine compounds have high water solubility and are easily migrated to the nearby soil and water environment, thus causing a certain degree of impact on the quality of the water environment

Method used

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  • Preparation method of load type biological iron-manganese composite oxide
  • Preparation method of load type biological iron-manganese composite oxide
  • Preparation method of load type biological iron-manganese composite oxide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The weight ratio of the raw material components for the preparation of the loaded biological iron-manganese composite oxide involved in this example is: 1-25 parts of Pseudomonas putida MnB1 bacteria liquid, 0.1-10 parts of manganese carbonate, 1 part of beef extract powder -5 parts, 2-10 parts of peptone, 1-5 parts of sodium chloride, 0.015-1.5 parts of ferrous ammonium sulfate, 0.0075-0.75 parts of yeast extract powder, 0.015-1.5 parts of sodium citrate, 0.005-0.5 parts of sodium pyrophosphate parts, 1000-3000 parts of deionized water; wherein the manganese carbonate is commercial manganese carbonate.

[0024] The method for using the loaded biological iron-manganese composite oxide to repair soil involved in this embodiment specifically includes the following steps:

[0025] (1), preparation of nutrient broth medium

[0026] Select 1-5g of beef extract powder, 2-10g of peptone, 1-5g of sodium chloride and 0.5-1.5L of deionized water and mix to obtain a nutrient brot...

Embodiment 2

[0034] (1) Source and culture of bacteria

[0035] The selected bacterial strain is Pseudomonas putida MnB1, which was purchased from the American Type Bacteria Collection Center, with a preservation number of ATCC 23483; the bacterial liquid was inoculated into 200ml of liquid nutrient broth medium with an inoculum of 3% by volume , the composition is: 3.0 g of beef extract powder, 10.0 g of peptone, 5.0 g of sodium chloride, 1 L of deionized water, pH=7.2, enrichment culture in a horizontal shaker at a temperature of 25° C. and a rotation speed of 150 rpm for 3 days;

[0036] (2) Preparation of supported biological iron-manganese composite oxides

[0037] Inoculate the bacterial liquid obtained by enrichment in step (1) into 200ml medium containing 0.15g of ferrous ammonium sulfate, 0.075g of yeast extract powder, sodium citrate, 0.15g, pyrophosphate Sodium, 0.05g, 1L of deionized water, in a polyvinyl chloride bottle with pH=6.8 aseptic stopper, add 0.8g commercial mangane...

Embodiment 3

[0040] (1) Source and culture of bacteria

[0041] The selected strain was Pseudomonas putida MnB1, which was purchased from the American Type Poison Collection Center with the accession number ATCC 23483. The bacterium liquid is inoculated into the liquid nutrient broth medium containing 200ml with an inoculation amount of 2% by volume, and consists of: beef extract powder 3.0g, peptone 10.0g, sodium chloride 5.0g, deionized water 1L, pH = 7.2, enrichment culture in a horizontal shaker with a temperature of 25°C and a rotation speed of 150 rpm for 2 days;

[0042] (2) Preparation of supported biological iron-manganese composite oxides

[0043] Inoculate the bacterial liquid obtained by enrichment in step (1) into 200ml medium containing 0.15g ferrous ammonium sulfate, 0.075g yeast extract powder, sodium citrate, 0.15g, pyrophosphate Sodium, 0.05g, 1L deionized water, pH = 6.8 sterile plugged polyvinyl chloride bottle, add 1g of synthetic manganese carbonate at the same time, ...

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Abstract

The invention relates to the restoration field of phenylarsonic acid pollutants, and concretely relates to a preparation method of load type biological iron-manganese composite oxide. The method mainly comprises the following steps: preparation of a nutrient broth medium, culture of flora, preparation of a biological manganese-iron medium, preparation of the load type biological iron-manganese composite oxide, and the like; Pseudomonas putida employs carbonate ore as an electron donor, a layered biological iron-manganese composite oxide is formed on the surface of manganese carbonate, and the load type biological iron-manganese composite oxide is obtained. The product can be used for fixing phenylarsonic acid compounds in soil and secondary products, such as arsenite and arsenate on the surface of mineral at the same time, in order to realize in-situ or non-in-situ restoration of phenylarsonic acid pollutants; the method is mainly suitable for soil with surface layer pollution, and the method has the advantages of simple operation, low treatment cost, and good effects without secondary pollution.

Description

Technical field: [0001] The invention relates to the field of remediation of phenylarsine pollutants, in particular to a preparation method of a loaded biological iron-manganese composite oxide. technical background: [0002] With the continuous growth of the world population and the improvement of residents' living standards, the global demand for food, especially protein, is becoming increasingly strong. Pork, eggs and chicken are important sources of protein for residents to obtain, and their sufficient output and low price are important guarantees for meeting the nutritional needs of the general public. Under the traditional breeding mode, the breeding cycle of pigs, broilers and laying hens is generally long, the breeding efficiency is low, and the breeding cost is high, which greatly restricts the output of the above products. Under the pressure of the above-mentioned severe situation and the drive of huge market interests, the application of intensive pig, meat poult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00B09C1/00C12R1/40
CPCC12P3/00B09C1/00
Inventor 王莹马文静迟庆东蔡冬石云鹏
Owner QINGDAO HAICHENG INTPROP SERVICES CO LTD
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