Method for detecting drug sensitivity rapidly

A drug-sensitivity and rapid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, measuring devices, etc., can solve the problems of long detection time, limited types of antibacterial drugs, unfavorable long-term detection of drug resistance, etc., and achieve simple operation Effect

Inactive Publication Date: 2019-10-08
AUTOBIO DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the detection method is simple to operate and the detection time is relatively shortened, the detection time is still very long for patients who need antibacterial infection treatment, so that the patients cannot be treated in time; at the same time, the existing automatic microbial drug sensitivity detection equipment can detect The types of antibacterial drugs in China are very limited, and the drug concentration range in the commercial drug sensitivity card matched with automatic detection equipment is small, which is not conducive to the long-term detection of drug resistance

Method used

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  • Method for detecting drug sensitivity rapidly
  • Method for detecting drug sensitivity rapidly
  • Method for detecting drug sensitivity rapidly

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 The method of the present invention was used to detect the resistance of Klebsiella pneumoniae (Klebsiella pneumoniae originated from a hospitalized patient A with pneumonia in a hospital, and the patient’s Klebsiella pneumoniae was cultured on a blood agar plate before the test) to meropenem The specific method is:

[0026] In the first step, according to CLSI's recommended cut-off point of Klebsiella pneumoniae to meropenem, MH broth was used to prepare a multiple gradient of meropenem dilution, the specific concentrations were 4μg / mL, 2μg / mL, 1μg / mL, use MH broth without meropenem as the blank control sample; the preparation time of the blank control sample and meropenem diluent can be controlled within 20 minutes;

[0027] The second step is to prepare Klebsiella pneumoniae standard bacteria suspension with ultrapure water, add ultrapure water to the turbidimetric tube during preparation, and pick the Klebsiella pneumoniae strain in the blood agar plate medium...

Embodiment 2

[0031] Example 2 The method of the present invention was used to detect the resistance of Klebsiella pneumoniae (Klebsiella pneumoniae was derived from a hospitalized patient B with pneumonia in a certain hospital, and the patient’s Klebsiella pneumoniae was cultured on a blood agar plate before the test) to meropenem The specific method is:

[0032] In the first step, according to the cut-off point value standard of Klebsiella pneumoniae to meropenem recommended by CLSI, MH broth is used to prepare a multiplier meropenem dilution, the specific concentrations are 4μg / mL, 2μg / mL, 1μg / mL , Use MH broth without meropenem as the blank control sample; the preparation time of the blank control sample and the meropenem diluent can be controlled within 20 minutes;

[0033] The second step is to prepare Klebsiella pneumoniae standard bacteria suspension with ultrapure water, add ultrapure water to the turbidimetric tube during preparation, and pick the Klebsiella pneumoniae strain in the bl...

Embodiment 3

[0038] Example 3 The method of the present invention was used to detect the resistance of Staphylococcus aureus (Staphylococcus aureus originated from a hospitalized patient C with pneumonia in a hospital, and the patient’s Staphylococcus aureus was cultured on a blood agar plate before the test) to oxacillin. The specific method is:

[0039] The first step is to mix MH broth and oxacillin according to the cut-off point value (ie resistance value, intermediate value, and sensitivity value) of oxacillin recommended by CLSI for Staphylococcus aureus to form a gradient of concentration Changed oxacillin diluent: 4μg / mL, 2μg / mL, use MH broth without oxacillin as a blank control; the first step can be controlled within 20min;

[0040] The second step is to prepare a standard bacterial suspension of Staphylococcus aureus with ultrapure water, add ultrapure water to the turbidimetric tube during preparation, and pick the Staphylococcus aureus strain in the blood agar plate medium into the...

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Abstract

The invention discloses a method for detecting drug sensitivity rapidly. The method includes the steps of 1, mixing nutrient broth with antibacterials to prepare an antibacterial diluent with gradientchange in concentration; 2, preparing a standard bacterial suspension of strain to be detected with super pure water, and diluting the broth for standby use; 3, respectively adding the standard bacteria suspension diluted by the broth of the same volume in the antibacterial diluent prepared in the step 1, dropwise adding the obtained mixture to a sample plate of a mass spectrometer for sample application according to the concentration gradient of the antibacterial diluent, and incubating for 2.0-5.0 hours after sample application is completed; 4, after incubation, discarding the broth, drying, dropwise adding a mass spectrometry identification pretreatment reagent, performing microbiological identification with the mass spectrometer after crystallization and reading the result.The pretreatment operation of the method is simple, accurate drug sensitivity result can be provided more quickly and intuitively, and a certain reference can be provided for clinical guidance and rational use of drugs in a timely manner.

Description

Technical field [0001] The invention relates to the field of drug susceptibility detection, in particular to a method for rapidly detecting drug susceptibility. Background technique [0002] Antibiotics (ie antibacterial drugs) have a wide range of clinical applications and have made great contributions to the prevention and treatment of human diseases. In recent years, with the increase in the types of antibiotics, clinical dependence on antibacterial drugs has become more and more serious. Due to the unreasonable use or abuse of antibiotics, bacteria have developed drug resistance, and the phenomenon of increasing drug resistance has seriously threatened human health. . The detection rate of various multi-drug-resistant bacteria and pan-drug-resistant bacteria continues to increase, and even super drug-resistant bacteria appear. The development speed of new drugs is far behind the production speed of drug-resistant bacteria, which has brought clinical anti-infection treatment....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/22C12R1/445
CPCC12Q1/18G01N2333/26G01N2333/31
Inventor 蔡克亚赵高岭刘美丽张婷婷张瑞峰
Owner AUTOBIO DIAGNOSTICS CO LTD
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