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Insoluble phosphate bioleaching method

A kind of insoluble phosphate, bioleaching technology, applied in the direction of phosphate, phosphorus oxyacid, bacteria, etc., can solve the problems of one month or even several months, slow decomposition of phosphate rock, weak organic acid acidity, etc. Achieve the effect of reducing the cost of grinding samples, improving the utilization rate and improving the conversion rate of phosphorus

Active Publication Date: 2017-01-04
SHENZHEN BATIAN ECOTYPIC ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned method has the following defects: (1) the leaching cycle is as long as one month or even several months, the cost is high, the inoculum size is generally 10% to 20%, and additional phosphorus compounds need to be added as the culture medium, the ore particle size is fine, and the pulp concentration is very high. Low, low resource utilization; (2) The organic acid produced by the metabolism of heterotrophic bacteria is not strong, and the decomposition rate of phosphate rock is slow, and organic nutrients need to be added as the carbon source required for growth, so the industrial application is limited; (3) ) Some fungi with strong phosphorus dissolving ability have the problems of high raw material production cost and easy inactivation of strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] S1, select medium and low-grade phosphate rock (total phosphorus content is about 18%), pulverize, pass 2mm sieve for subsequent use;

[0037] S2. Strain screening:

[0038] S21. Preparation of phosphorus-dissolving circle medium: Weigh 10 g of glucose, 0.5 g of ammonium sulfate, 0.3 g of potassium chloride, 10 g of insoluble phosphate, and 17 g of agar, add 1 L of water, mix, and culture at a temperature of about 30°C for 7 sky;

[0039] S22. Preparation of liquid shake flask culture medium: Weigh 10 g of glucose, 0.5 g of ammonium sulfate, 30 g / L of insoluble phosphate and add 1 L of water. The culture temperature of the shake flask is 30° C., the speed of the shaker is 100 r / min, and the culture time is 7 days.

[0040] S23. Adding the jelly-like Bacillus strain into the above-mentioned medium phosphorus-dissolving ring and liquid shake flask medium for qualitative and quantitative determination, and screening out high-efficiency strains with strong phosphorus-dissolv...

Embodiment 2

[0053] S1, select medium and low-grade phosphate rock (total phosphorus content is about 22%), pulverize, cross 2mm sieve for subsequent use;

[0054] S2. Strain screening:

[0055] S21. Preparation of phosphorus-dissolving circle medium: Weigh 15g of glucose, 0.8g of ammonium sulfate, 0.5g of potassium chloride, 15g of insoluble phosphate, 18g of agar, add 1L of water, mix, and culture at a temperature of about 35°C for 8 sky;

[0056] S22. Preparation of liquid shake flask culture medium: Weigh 15 g of glucose, 0.8 g of ammonium sulfate, 35 g / L of insoluble phosphate and add 1 L of water. The culture temperature of the shake flask is 30° C., the rotation speed of the shaker is 100 r / min, and the culture time is 8 days.

[0057] S23, adding the Bacillus megaterium strain into the above-mentioned medium phosphorus-dissolving circle and the liquid shake flask medium for qualitative and quantitative determination, and screening out high-efficiency strains with strong phosphorus...

Embodiment 3

[0064] S1, select middle and low grade phosphate rock (total phosphorus content is about 24%), pulverize, cross 2mm sieve for subsequent use;

[0065] S2. Strain screening:

[0066] S21. Preparation of phosphorus-dissolving circle medium: Weigh 5 g of glucose, 0.3 g of ammonium sulfate, 0.3 g of potassium chloride, 8 g of insoluble phosphate, and 16 g of agar, add 1 L of water, mix, and culture at a temperature of about 32°C for 6 sky;

[0067] S22. Preparation of liquid shake flask culture medium: Weigh 5 g of glucose, 0.3 g of ammonium sulfate, 25 g / L of insoluble phosphate and add 1 L of water. The culture temperature of the shake flask is 30° C., the rotation speed of the shaker is 100 r / min, and the culture time is 6 days.

[0068] S23, adding the Bacillus megaterium strain into the above-mentioned medium phosphorus-dissolving circle and the liquid shake flask medium for qualitative and quantitative determination, and screening out high-efficiency strains with strong pho...

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Abstract

An insoluble phosphate bioleaching method is characterized in that insoluble phosphate raw materials are crushed and sieved by a 2mm sieve, bacillus is qualitatively and quantitatively measured by a phosphate dissolving ring and a liquid shake flask culture medium, efficient strains with high phosphate dissolving capacity is screened, 2-8ml screened efficient strains are inoculated into 100ml of nutrient broth media and cultured for 3-5 days under the condition of 25-35 DEG C and 100-150r / min to serve as bacteria, 10% of sucrose is added into clear water and serves as a nutrient source, 1-7% of insoluble phosphate raw materials are added, 2-8% of the cultured bacteria are inoculated and cultured for 7-10 days under the condition of 25-35 DEG C and 120-160r / min, and solid residues and culture solution are separated until the content of water-soluble phosphorus in the culture solution is higher than or equal to 100mg / L. The selected bacteria have an efficient phosphate dissolving function and are high in phosphate bioleaching rate, the content of beneficial microorganisms in soil can be increased, and phosphorus use rate is increased.

Description

technical field [0001] The invention belongs to the technical field of using microorganisms to treat mineral resources, in particular to a biological leaching method for converting phosphorus in phosphate rock into soluble phosphorus. Background technique [0002] Phosphorus is an important chemical raw material and a necessary element for the growth of crops. A large amount of phosphorus for industrial use must be extracted from phosphate rock. Although my country has abundant phosphate rock resources, the proven resource reserves are 13.24 billion tons, but the average grade is only 16.95%. (in P 2 o 5 count), and the current industrial production of phosphoric acid requires phosphorus ore to contain P 2 o 5 ≥28%. Therefore, how to make full use of low-grade phosphate rock resources has become a task with strategic significance for sustainable development. [0003] With the rapid development of biotechnology, the use of bacteria to leach low-grade metal sulfide mineral...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B25/26C12N1/20
CPCC01B25/26C12N1/20
Inventor 黄春艳穆光远韦春近杜怡然
Owner SHENZHEN BATIAN ECOTYPIC ENG
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