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A kind of Enterobacteriaceae strain and its application in promoting the growth of Phyllostachys pubescens

A technology of Enterobacteriaceae and Phyllostachys pubescens, applied in the application, plant growth regulator, plant growth regulator and other directions, to achieve the effect of strong potassium solubilization ability, strong auxin secretion ability, good potassium solubilization and auxin IAA secretion ability

Active Publication Date: 2020-05-05
JIANGXI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the research on the growth-promoting bacteria in the rhizosphere of Phyllostachys pubescens

Method used

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  • A kind of Enterobacteriaceae strain and its application in promoting the growth of Phyllostachys pubescens
  • A kind of Enterobacteriaceae strain and its application in promoting the growth of Phyllostachys pubescens
  • A kind of Enterobacteriaceae strain and its application in promoting the growth of Phyllostachys pubescens

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Experimental program
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Embodiment 1

[0024] From the rhizosphere soil of 5-year-old Moso bamboo in Dagang Forest Farm, Yifeng County, Yichun City, Jiangxi Province, a strain of Enterobacter soli RW37, which can promote the growth of Moso bamboo, was screened and obtained, and the strain number was RW37. The main biological characteristics of this Enterobactersoli (Enterobactersoli) RW37: non-spore-forming Gram-negative (G-) short bacilli, colonies are round, with neat edges, and the surface is moist and sticky ( figure 1 ). The strain is milky white, positive for contact enzyme, negative for oxidase, positive for 3% potassium hydroxide test, positive for starch hydrolysis test, positive for gelatin hydrolysis test, positive for nitric acid reduction test, positive for methyl red, negative for VP, and negative for indole. The 16S rDNA sequence of Enterobacter soli RW37 strain is shown in SEQ ID NO:1. The measured 16S rDNA sequence was compared with the sequence in the GeneBank database ( figure 2 ). The result...

Embodiment 2

[0030] Example 2 Determination of the Phosphorus Solubilizing Ability of Enterobacter RW37 at Different Initial pH

[0031] 1. Detection of Phosphorus Solubilizing Ability of Enterobacter RW37 to Insoluble Phosphate

[0032] Phosphate Solubilizing Medium A: Glucose 10g, Ca 3 (PO 4 ) 2 5g, MgCl 2 5g, KCl 0.2g, MgSO 4 .7H 2 O 0.25g, (NH 4 ) 2 SO 4 0.1g, distilled water 1000mL, mix well and sterilize.

[0033] Phospholytic medium B: aluminum phosphate (AlPO 4 ) instead of Ca in phosphate-dissolving medium A 3 (PO4) 3 (the amount is also the same), and the other components and contents are the same, mixed evenly and sterilized.

[0034] Phospholytic medium C: ferric phosphate (FePO 4 ) instead of Ca in phosphate-dissolving medium A 3(PO 4 ) 3 (the amount is also the same), and the other components and contents are the same, mixed evenly and sterilized.

[0035] Phosphate-dissolving medium D: calcium hydrogen phosphate (CaHPO 4 ) instead of Ca in phosphate-diss...

Embodiment 3

[0042] Example 3 Determination of Enterobacter RW37 Potassium Solubilizing Ability

[0043] Enterobacter RW37 was activated according to the method of Example 2. Enter the activated enterobacteria RW37 into 50mL potassium-dissolving medium (the formula of the potassium-dissolving medium is: potassium feldspar powder 0.1g, Na 2 HPO 4 2.0g, FeCl 3 0.005g, MgSO 4 ·7H 2 O0.5g, sucrose 5.0g, Ca 2 CO 3 0.1g, distilled water 1000mL, pH 7.0, mixed uniformly and sterilized) in a 100mL Erlenmeyer flask, vibrated at 28°C and 180r / min for 7 days to obtain a fermentation broth, and the fermentation broth was first 500r / min, 10min Remove the insoluble substances in the fermentation broth, then collect the supernatant by centrifugation under the condition of 10000r / min for 10min, and measure the content of available potassium in the supernatant by flame spectrophotometer. And the potassium solution medium inoculated with the same volume of blank seed solution was used as the contro...

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Abstract

The invention discloses an enterobacteria strain and its application in promoting the growth of moso bamboo. The taxonomic name of the strain is Enterobacter soli, the strain number is RW37, and it is preserved in the China Center for Type Culture Collection with the preservation number: CCTCC NO: M 2018355. The Enterobacter soli RW37 screened from the rhizosphere soil of Moso bamboo in the present invention has the ability to efficiently decompose phosphorus, potassium and secrete IAA; at different initial pHs, it has good dissolving capacity for 5 different mineral phosphates; in addition, The liquid bacterial agent prepared by the enterobacterium RW37 of the present invention has significant growth-promoting effect on the ground diameter and seedling height of moso bamboo. Therefore, the present invention provides excellent bacterial strain resources for developing environment-friendly moso bamboo biological special fertilizers.

Description

technical field [0001] The invention relates to the field of microorganisms and biological fertilizers, in particular to an enterobacteriaceae strain and its application in promoting the growth of moso bamboo. Background technique [0002] Moso bamboo (Phyllostachys edulis) is a typical uniaxial scattered clonal plant with strong vegetative and reproductive ability. It is the most widely distributed among bamboo plants in my country, has the largest stock volume, and is the most widely used. It is economically and ecologically beneficial. It is the best bamboo species and the most important timber production species after masson pine and Chinese fir. In recent years, with the increase of the demand for moso bamboo, the phenomenon of nutrient depletion in moso bamboo plantations has also increased day by day, which has threatened the sustainable management and utilization of bamboo forests. At present, weak soil fertility and insufficient nutrient supply are one of the main p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20C09K17/14A01P21/00C05F11/08C12R1/01C09K101/00
CPCA01N63/00C05F11/08C09K17/14C09K2101/00C12N1/205C12R2001/01
Inventor 张扬陈伏生方向民张林平万松泽郭春兰卜文圣胡小飞李冬
Owner JIANGXI AGRICULTURAL UNIVERSITY
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