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A kind of basket-shaped golden bacterium and its application

A technology of Talaromyces aureus and golden yellow, applied to Talaromyces aureus and its application fields, can solve problems such as screening and application research that have not been reported, and achieve the effect of the ability to understand potassium and secrete IAA.

Active Publication Date: 2020-11-24
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the screening and application of strains that have the functions of dissolving phosphorus, potassium and secreting IAA in the rhizosphere of Phyllostachys pubescens

Method used

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  • A kind of basket-shaped golden bacterium and its application
  • A kind of basket-shaped golden bacterium and its application
  • A kind of basket-shaped golden bacterium and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: JXBR04 bacterial strain growth test under acid stress

[0027] Inoculate the activated bacterial strain JXBR04 into PDA liquid medium (the formula of PDA liquid medium is: potato 200g, glucose 20g, distilled water 1000mL), 25 ℃, 120r / min shaking culture 5d as seed liquid, the pH of PDA liquid medium Set to six gradients of 1.5, 2.5, 3.5, 4.5, 5.5, and 6.5, and distribute them in 250ml Erlenmeyer flasks with a liquid volume of 100mL. Subsequently, the seed solution was inoculated into the PDA liquid medium according to the inoculum amount of 1% (v / v), and each treatment was repeated three times, and the culture was shaken at 25°C and 120r / min for 8 days. After the mycelia were filtered, they were dried at 65°C for 48 hours and weighed. The results are shown in Table 1. The strain JXBR04 grew better under different acidic conditions of pH 1.5, 2.5, 3.5, 4.5, 5.5 and 6.5, and there was no difference in mycelium biomass, and the pH range of the fermentation b...

Embodiment 2

[0031]Example 2: Phosphorus solubilization test of strain JXBR04 under acid stress.

[0032] Phosphate Solubilizing Medium A: Glucose 10g, Ca 3 (PO 4 ) 2 5g, MgCl 2 5g, KCl 0.2g, MgSO 4 .7H 2 O 0.25g, (NH 4 ) 2 8O 4 0.1g, distilled water 1000mL.

[0033] Phospholytic medium B: aluminum phosphate (AlPO 4 ) instead of Ca in phosphate-dissolving medium A 3 (PO4) 3 , the other components and contents are the same.

[0034] Phospholytic medium C: ferric phosphate (FePO 4 ) instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 3 , the other components and contents are the same.

[0035] Phosphate-dissolving medium D: calcium hydrogen phosphate (CaHPO 4 ) instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 3 , the other components and contents are the same.

[0036] Phosphate-dissolving medium E: calcium phytate (C 6 h 6 Ca 6 o 24 P 6 ) instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 3 , the other components and contents are the same. ...

Embodiment 3

[0039] Example 3: Determination of the potassium-dissolving ability of the JXBR04 strain.

[0040] Insert the JXBR04 bacterial strain into 50mL potassium-dissolving medium (the formula of the potassium-dissolving medium is: potassium feldspar powder 0.1g, Na 2 HPO 4 2.0g, FeCl 3 0.005g, MgSO 4 ·7H 2 O 0.5g, sucrose 5.0g, Ca 2 CO 3 0.1g, 18.0g agar, 1 000mL distilled water, pH 7.0. ) in a 100mL Erlenmeyer flask, and vibrated at 28°C and 180r / min for 7 days to obtain a fermentation broth. The fermentation broth was first removed at 500r / min for 10min to remove insoluble substances in the fermentation broth, and then at 10000r / min for 10min. The supernatant was collected by centrifugation, and the content of available potassium in the supernatant was determined by a flame spectrophotometer. The result is as Figure 5 As shown, JXBR04 has a potassium-dissolving capacity of 15.28mg / L, which has a good potassium-dissolving ability.

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Abstract

The invention discloses a golden basket-shaped fungus, which is classified as a golden basket-shaped fungus ( Talaromyces aurantiacus ), the strain number is JXBR04, and it is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2017327, and the preservation date is June 13, 2017. The golden basket fungus screened from the rhizosphere soil of moso bamboo in the present invention not only has a high-efficiency phosphorus-dissolving function, but also has a better ability of dissolving potassium and secreting IAA. Under the conditions of liquid shaking culture, it has a strong dissolving ability for insoluble phosphate, and promotes the transformation of insoluble phosphorus into soluble nutrients; The liquid bacterial agent prepared by the basket-shaped bacteria has significant growth-promoting effect on the ground diameter and seedling height of moso bamboo. Therefore, the invention provides excellent bacterial strain resources for the development of environment-friendly bio-special fertilizer for moso bamboo.

Description

technical field [0001] The invention relates to the field of microorganisms and biological fertilizers, and relates to a golden basket bacterium and an application thereof. Background technique [0002] Moso bamboo (Phyllostachys edulis) is an economic tree of the Gramineae bamboo subfamily. According to incomplete statistics, my country's existing moso bamboo forest exceeds 3.8 million ha, accounting for 70% of the national bamboo forest area and 80% of the global moso bamboo area. In many mountainous areas in southern my country, moso bamboo has become the main economic forest species for agricultural and forestry industry structure adjustment, forestry efficiency enhancement, and forest farmers' income increase. However, most of the moso bamboo forest management is extensive at present. Insufficient nutrient supply, especially the lack of phosphorus, has become one of the main problems in the cultivation of moso bamboo resources; the application of chemical fertilizers ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P21/00C05G3/00C05G5/20C05F11/08C12R1/645
CPCA01N63/30C05G3/00C12N1/14C12N1/145C12R2001/645
Inventor 陈伏生张扬张林平栾丰刚万松泽方向民胡小飞李冬
Owner JIANGXI AGRICULTURAL UNIVERSITY
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