Microbial reinforcement liquid for slope reinforcement and preparation method and construction method of microbial reinforcement liquid

A construction method and reinforcement liquid technology, applied in the field of microbial reinforcement liquid and microbial reinforcement liquid for slope reinforcement, can solve the problems of large degree of environmental damage, high cost, secondary slump, etc., to improve utilization rate and inhibit pathogenicity The effect of bacterial growth and uniform distribution of reinforcement solution

Pending Publication Date: 2021-01-19
哈尔滨工大岩土工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method of slope protection is expensive, the degree of environmental damage is large, and it is easy to cause secondary landslides

Method used

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  • Microbial reinforcement liquid for slope reinforcement and preparation method and construction method of microbial reinforcement liquid
  • Microbial reinforcement liquid for slope reinforcement and preparation method and construction method of microbial reinforcement liquid
  • Microbial reinforcement liquid for slope reinforcement and preparation method and construction method of microbial reinforcement liquid

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specific Embodiment approach 1

[0033] Specific embodiment 1: This specific embodiment discloses a microbial reinforcement solution for slope reinforcement, including an independently separated adhesive binder, mineralization reinforcement fluid A and mineralization reinforcement fluid B, and the adhesion binder is sodium Ionic solution, the mineralization reinforcement solution A includes independently separated mineralization bacteria solution A and mineralization bacteria nutrient solution A, the strains of the mineralization bacteria solution A are Bacillus subtilis and Bacillus licheniformis, and the mineralization bacteria solution A's OD 600 value between 1.0 and 1.5, the mineralizing bacteria nutrient solution A includes distilled water, 3 to 5 g / L nutrient broth, 35 to 45 g / L urea, 65 to 85 g / L calcium chloride and 10 to 20 g The ammonium chloride of / L, preferably distilled water, the nutrient broth of 4g / L, the urea of ​​40g / L, the calcium chloride of 75g / L and the ammonium chloride of 15g / L, desc...

specific Embodiment approach 2

[0034] Embodiment 2: This embodiment is a further description of Embodiment 1. The adhesive is a sodium chloride solution with a sodium ion concentration of 0.8 to 1.2 mol / L, preferably a sodium ion concentration of 1.0 mol / L of sodium chloride solution.

specific Embodiment approach 3

[0035] Embodiment 3: This embodiment discloses a method for preparing a microbial reinforcement solution for slope reinforcement according to Embodiment 1, including:

[0036] The preparation method of mineralizing bacteria solution A: inoculate Bacillus subtilis and Bacillus licheniformis into No. 1 medium after activation, shake and culture at room temperature for 1-2 days, remove the original medium after the expansion is completed, and use fresh No. 1 medium The culture medium dilutes the bacterial liquid, and the obtained bacterial liquid is the mineralized bacterial liquid A. Among them, the No. 1 medium consists of distilled water, 15-25g / L glucose, 15-25g / L agar, peptone, 4-6g / L sodium chloride and 0.1-0.2g / L sodium hydroxide, preferably distilled water, 20g / L glucose, 20g / L agar, 8g / L peptone, 5g / L Sodium chloride and 0.2g / L sodium hydroxide;

[0037]The preparation method of mineralizing bacteria solution B: activate Sarcina and Rhodopseudomonas sphaeroides and inoc...

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Abstract

The invention discloses a microbial reinforcement liquid for slope reinforcement, a preparation method and a construction method thereof, and relates to a microbial reinforcement liquid. The adhesivebinder is a sodium ion solution, strains of the mineralized bacteria solution A are bacillus subtilis and bacillus licheniformis, the OD600 value is 1.0-1.5, the mineralized bacteria solution A comprises distilled water, 3-5g/L of nutrient broth, 35-45g/L of urea, 65-85g/L of calcium chloride and 10-20g/L of ammonium chloride, and strains of the mineralized bacteria solution B are sporosarcina pasteurii and rhodopseudomonas; the OD600 value ranges from 0.5 to 1.0; the mineralized bacteria nutrient solution B comprises distilled water, 4-6g/L of nutrient broth, 25-35g/L of urea, 45-65g/L of calcium chloride and 10-20g/L of ammonium chloride. The mineralization ability of bacillus subtilis and sporosarcina pasteurii is utilized to carry out ecological reinforcement on the expansive soil slope.

Description

technical field [0001] The invention relates to a microbial reinforcement liquid, in particular to a microbial reinforcement liquid for slope reinforcement, a preparation method and a construction method thereof, belonging to the technical field of slope engineering. Background technique [0002] Expansive soil is rich in clay minerals and has the characteristics of swelling when it encounters water and shrinking when it loses water. It is prone to cracks under freeze-thaw and dry-wet cycles. Under rainfall and snowmelt infiltration, snow water enters the cracks, causing expansive soil Swelling occurs on the surface of the slope, which reduces the strength of the soil and induces shallow landslides. The traditional method of slope protection is expensive, causes great damage to the environment, and easily causes secondary landslides. [0003] Microbe-induced calcium carbonate deposition technology has always been a research hotspot of researchers at home and abroad, and thi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/50C09K17/14E02D17/20E02D3/12A01N63/22A01P21/00C05G3/00C05G3/80C12N1/20C12R1/01C12R1/125C12R1/10C09K103/00
CPCC09K17/50C09K17/14E02D17/20E02D3/12A01N63/22C05G3/00C05G3/80C12N1/20C09K2103/00
Inventor 丛晟亦朱磊胡意如邢文强程志和张森
Owner 哈尔滨工大岩土工程技术有限公司
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