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Method for treating mercury in water with escherichia coli

A technology for Escherichia coli and water treatment, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc. simple effect

Inactive Publication Date: 2016-04-20
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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Problems solved by technology

[0009] It is precisely because of the pollution caused by the large-scale use of mercury that it has caused serious mercury pollution

Method used

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  • Method for treating mercury in water with escherichia coli

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Embodiment

[0020] a, strain source and culture: the selected facultative anaerobic bacterium Escherichia coli, preservation number: GIM1.251, purchased from Guangdong Microbial Strain Collection Center (GIM), inoculated Escherichia coli into peptone 10‰, beef extract 1.5 ‰, yeast extract powder 1.5g / L and sodium chloride 5‰ nutrient broth medium, cultured under anaerobic conditions at a temperature of 20-30°C for 8-15 hours to the stable growth period, and added 0.1-1mmol / L at the same time Sodium selenite or sodium citrate as electron donor, with N 2 / H 2 As a gas to maintain anaerobic environment, the anaerobic environment is provided by DWSDG250 anaerobic workstation;

[0021] b. Purification process: Add the inoculum of Escherichia coli in the stable growth phase of step a to the water containing divalent mercury by volume ratio of 10%-30%, the initial concentration of divalent mercury in the water is 40-200 μg / L, and add The nutrient broth of 10-20g / L is peptone 10‰, beef extract ...

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Abstract

The invention relates to a method for treating mercury in water with escherichia coli. According to the method, escherichia coli is used and cultured in an enrichment mode in a nutrient broth medium to a stable growth period on the anaerobic condition, then the water containing divalent mercury is inoculated with escherichia coli, nutrient broth serves as nutrition to maintain bacterium growth, and anaerobic culture is carried out for several days on the room temperature condition to stop the experiment. The divalent mercury ions (Hg2+) in the sewage can be converted into mercuric chloride (HgCl) precipitate by escherichia coli, and mercuric chloride (HgCl) precipitate is difficult to dissolve in water, can stably exist in a water body and finally deposits to the bottom of the water body, so that divalent mercury pollution in the water body is removed, harm to the human body is reduced, and secondary pollution is avoided. The method has the advantages of being low in cost, wide in application range and the like.

Description

technical field [0001] The invention relates to a method for treating mercury in water by using Escherichia coli, and belongs to the technical field of sewage purification and treatment in the field of environmental protection. Background technique [0002] Mercury, commonly known as mercury, is the only liquid heavy metal element that exists under normal temperature and pressure conditions. Some people list mercury as one of the three most dangerous metal elements, and the U.S. Environmental Protection Agency lists mercury as one of 129 hazardous chemicals, mainly because of its high toxicity, persistence, bioaccumulation, and long-distance transmission specialty. [0003] Mercury is biologically toxic. Mercury is a highly toxic non-essential element that has no beneficial biological functions for living organisms. A small amount of mercury entering the human body will not cause harm to the human body, and will be excreted through feces, urine, sweat and other channels. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/20
CPCC02F3/34C02F2101/20
Inventor 潘响亮王潇男宋文娟
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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