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Method for extracting flavonoids compound from zostera marina

A technology of flavonoids and flavonoids of macrophylla, which is applied in the directions of plant/algae/fungus/moss components, pharmaceutical formulations, plant raw materials, etc., can solve the problems of long purification cycle, etc., and achieves shortened purification cycle, easy solvent, and increased content Effect

Inactive Publication Date: 2015-12-23
OCEAN UNIV OF CHINA
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  • Summary
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AI Technical Summary

Problems solved by technology

The content of flavonoids in the eosinopsis after purification by macroporous resin reaches about 51%, and the whole purification cycle is about 4 hours, which is longer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Step 1: Extraction of Fusarium flavonoids

[0019] Dry and grind Zinariae into powder and pass through a 40-mesh sieve, add 70% (V / V) ethanol at a solid-liquid ratio of 1:16, add cellulase at 0.025g / g raw material (Zincinia), Adjust the pH to 5, react at 45°C for 30min, then place it in an ultrasonic device with a power of 250W for 15min, and then place it at 55°C for 3h. The extract is concentrated and dried to obtain the crude extract of the flavonoids of the eosinopsis.

[0020] With rutin as standard, the content of flavonoids was determined by sodium nitrite-aluminum nitrate colorimetric method. The rutin standard curve equation obtained through regression is A=11.8735c+0.0055, and the linear correlation coefficient R 2 = 0.9994. Weigh and dissolve an appropriate amount of flavonoids from eosinopsis, after color development by sodium nitrite-aluminum nitrate, measure the absorbance at 510nm, bring it into the standard curve equation to obtain the concentration o...

Embodiment 2

[0026] Step 1: Extraction of Fusarium flavonoids

[0027] Dry and grind the Zelpium japonicus into powder and pass through a 40-mesh sieve, add 70% (V / V) ethanol at a solid-liquid ratio of 1:16, add cellulase at 0.025 g / g of raw materials, and adjust the pH to 5. React at 45°C for 30 minutes, then place in an ultrasonic device with a power of 250W for 15 minutes, and then place at 55°C for leaching for 3 hours. The extract is concentrated and dried to obtain the crude extract of the flavonoids of the eosinopsis.

[0028] With rutin as standard, the content of flavonoids was determined by sodium nitrite-aluminum nitrate colorimetric method. The rutin standard curve equation obtained through regression is A=11.8735c+0.0055, and the linear correlation coefficient R 2 = 0.9994. Weigh and dissolve an appropriate amount of flavonoids from eosinopsis, after color development by sodium nitrite-aluminum nitrate, measure the absorbance at 510nm, bring it into the standard curve equat...

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Abstract

The invention relates to a method for extracting a flavonoids compound from zostera marina. According to the method provided by the invention, the zostera marina is taken as a raw material, the extracted flavonoids compound and metal salt are enabled to be in reaction, stirring is performed, temperature and pH value are adjusted, after the reaction, precipitates are collected and dissolved into an ethanol solution, then an EDTA decomplexing mixture is added for oscillating reaction, rotating and drying by distillation are performed, then methyl alcohol is added for dissolving and filtering, and then filtrate concentration and drying are performed, namely the flavonoids compound of the zostera marina can be obtained after purification. The method provided by the invention can enable the content of the flavonoids compound of the zostera marina to reach more than 55 percent. According to the method provided by the invention, the technological process is simple, the operation period is short, the raw material is easy to access, solvent recovery is easy, energy consumption is low, and the method is a purification method for the flavonoids compound and has broad prospects for development.

Description

technical field [0001] The invention belongs to the technical field of purification of flavonoids, in particular to a purification method for extracting flavonoids from eel algae. Background technique [0002] Zostera marina (Zosteramarina L.) is a perennial seaweed belonging to the phylum Angiosperms, the class Monocots, and the order Marzophytes, and belongs to the genus Zosteramarina of the family Oculiaceae. The eelgrass, eelgrass latifolia, eelgrass clump, eelgrass and dwarf eelgrass in the genus eel are all distributed in our country. Among them, eelgrass is mainly distributed in the coastal areas of Liaoning (Dalian, Xingcheng and Suizhong), Shandong (Qingdao, Changdao, Rongcheng, Shidao and Rushan) and Hebei (Beidaihe) in China. As a higher plant that grows in a salty water environment, eelgrass' unique growth environment determines that there must be many biologically active substances in its body. Flavonoids are one of the active substances, which have various bi...

Claims

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Application Information

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IPC IPC(8): A61K36/88
Inventor 张朝辉李静应锐段筱杉李八方
Owner OCEAN UNIV OF CHINA
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