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Zostera marina somatic embryo induction method

A technology of somatic embryos and macrophylla, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as patents and literature that have not been reported, and achieve improved surface disinfection effect, short acquisition time, and avoidance of Effects of risk of genetic mutation in cells

Inactive Publication Date: 2016-03-16
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention induces its somatic embryos through the tissue culture technique of Z. echinacea. At present, no relevant patents and documents using this technical solution have been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: a method for inducing eosinophila somatic embryos, its implementation steps include:

[0017] (1) Selection of explants: take the flower branch of Eelium eosinophila as the explant donor, take the healthy young spathe inflorescence, scrape off the attached organisms on the surface, wash it in sea water and transfer it to the ultra-clean workbench Inside, soak in 10% hydrogen peroxide solution for 15-20min, rinse with sterilized sea water for 3 times, peel off the bract leaves outside the spathe inflorescence to obtain a sterile inflorescence;

[0018] (2) Induction culture of somatic embryos: Cut the sterile inflorescences into 3-5mm long explant fragments, inoculate them in the induction medium, and cultivate them at 16°C, under dark conditions and under 1000lux light conditions, every half a month Transfer once. After 17 days, small protrusions could be seen on the inflorescence, and after about 30 days, these small protrusions could be seen to develop ...

Embodiment 2

[0019] Embodiment 2: a kind of induction method of eosinopsis somatic embryo, its implementation steps are:

[0020] (1) Selection of explants: take the flower branch of Eelium eosinophila as the explant donor, take the healthy young spathe inflorescence, scrape off the attached organisms on the surface, wash it in sea water and transfer it to the ultra-clean workbench Inside, soak in 10% hydrogen peroxide solution for 15-20min, rinse with sterilized sea water for 3 times, peel off the bract leaves outside the spathe inflorescence to obtain a sterile inflorescence;

[0021] (2) Induction culture of somatic embryos: Cut the sterile inflorescences into 3-5mm long explant fragments, inoculate them in the induction medium, culture them at 16-20°C in the dark, and transfer them every half a month . After 20 days, small protrusions can be seen on the inflorescence, and after about 30 days, these small protrusions can be seen to develop into somatic embryos, which do not go through ...

Embodiment 3

[0022] Embodiment 3: a kind of induction method of eosinopsis somatic embryo, its implementation steps are:

[0023] (1) Selection of explants: take the flower branch of Eelium eosinophila as the explant donor, take the healthy young spathe inflorescence, scrape off the attached organisms on the surface, wash it in sea water and transfer it to the ultra-clean workbench Inside, soak in 10% hydrogen peroxide solution for 15-20min, rinse with sterilized sea water for 3 times, peel off the bract leaves outside the spathe inflorescence to obtain a sterile inflorescence;

[0024] (2) Induction culture of somatic embryos: Cut the sterile inflorescences into 3-5mm long explant fragments, inoculate them in the culture medium, culture them at 16-20°C in the dark, and transfer them every half month. After 23 days, small protrusions could be seen on the inflorescence, and after about 34 days, these small protrusions could be seen to develop into somatic embryos, which did not go through t...

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Abstract

The present invention relates to a zostera marina somatic embryo induction method, which comprises that the spadix of zostera marina is adopted as an explant, the inflorescence being subjected to a surface disinfection treatment is cut into fragments with a length of 3-5 mm, the fragment is inoculated into a somatic embryo induction culture medium, induction culture is performed at a temperature 15-25 DEG C under a dark or weak illumination condition, transfer inoculation is performed every two weeks, tissue sprouting can be generated on the inflorescence after culture for 15-25 d, small enation is generated to gradually develop into the somatic embryo, and during the process, the callus stage does not exist, wherein the somatic embryo induction culture medium is a solid culture medium adopting a MS culture medium and a N6 culture medium as a base culture medium and added with an organic additive, a carbon source, plant hormones (auxin or auxin and cytokinin), and sea salt. According to the present invention, the callus induction culture stage does not exist, and the zostera marina somatic embryo is directly induced from the inflorescence tissue within the short time, such that the important technical support is provided for the zostera marina sterile tissue culture seedling regeneration and the tissue culture rapid propagation system establishing, and the foundation is established for the successful seaweed tissue culture.

Description

technical field [0001] The invention belongs to the field of plant tissue culture research, and the invention relates to a method for inducing somatic embryos of eosinopsis. Background technique [0002] Eelweed ( Zostera marina L.) is the most widely distributed seagrass in the temperate seas of the northern hemisphere, and it is also the dominant species in seagrass farms in northern my country (Shandong, Hebei and Liaoning). It has extremely important ecological service functions and economic value. However, in the past hundred years, the global eelgrass seagrass ecosystem has been seriously damaged, and the area has shrunk sharply, even worse in my country. Therefore, in recent years, the restoration of eelgrass seagrass has become an indispensable item in the restoration of marine ecosystems in northern my country. Contents, a large number of seedlings are needed in the grassland restoration, and using plant tissue culture technology to cultivate sterile seedlings indoo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 郑凤英刘雪芹金艳梅张伟韩晓弟李乐乐
Owner SHANDONG UNIV
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