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Method for quantitating benzo (a) pyrene by adopting non-carcinogen anthracene as standard

A technology for carcinogens and benzos, which is applied in the field of quantitative detection of benzopyrene using non-carcinogenic anthracene as a standard solution, can solve problems such as increased exposure to benzos and human health threats, and achieve reduction in probability, health risks, Inexpensive effect

Active Publication Date: 2013-10-09
XIAMEN UNIV
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  • Claims
  • Application Information

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Problems solved by technology

For researchers who have been engaged in the analysis and research of polycyclic aromatic hydrocarbons such as benzo(a)pyrene for a long time, this undoubtedly increases the probability of exposure to benzo(a)pyrene, which poses a potential threat to the health of relevant personnel

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  • Method for quantitating benzo (a) pyrene by adopting non-carcinogen anthracene as standard
  • Method for quantitating benzo (a) pyrene by adopting non-carcinogen anthracene as standard
  • Method for quantitating benzo (a) pyrene by adopting non-carcinogen anthracene as standard

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: the detection of benzo(a)pyrene in artificially synthesized samples

[0035] Pipette an appropriate amount of solution containing 1.0ng / mL benzo(a)pyrene and measure the constant energy simultaneous fluorescence spectrum on a fluorometer, with an energy difference of 700cm -1 , and then continuously add 10, 20, 30ng / mL anthracene standard solutions to the measurement solution, and draw the synchronous fluorescence spectrum (such as figure 1 Show). Read the fluorescence intensity of anthracene at 374nm, draw the working curve with the intensity to the standard concentration, and get the slope (such as figure 2 Show). Read the fluorescence intensity of benzo(a)pyrene at 392nm as 38.9, and substitute the slope 1.89, fluorescence intensity 38.9 and relative correction factor 0.050 into the quantitative formula proposed by the present invention to calculate the concentration of benzo(a)pyrene as 1.03ng / mL, Consistent with the reference value (1.00ng / mL).

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Embodiment 2

[0037] Embodiment 2: the detection of benzo (a) pyrene in edible oil

[0038] Weigh 0.5g blended oil sample into a 25mL serum bottle, add 4mL dimethyl sulfoxide solvent, extract under 59KHz, 250W ultrasonic conditions for 6min, take out and let stand to separate layers, and then extract the separated upper oil layer under the same conditions Once, the dimethyl sulfoxide extract was collected twice as the test solution. Pipette 2mL of the solution to the conventional quartz fluorescence sample cell of the fluorescence photometer, and draw the first-order derivative nonlinear variable-angle simultaneous fluorescence spectrum (such as Figure 4 shown). Then continuously add the standard solution of 10, 20, 30ng / mL anthracene in the sample measuring solution, and draw the synchronous fluorescence spectrum (such as Figure 5 shown). Read the fluorescence intensity of anthracene, draw the working curve with intensity versus concentration, and the slope is 37.98. Reading the benz...

Embodiment 3

[0039] Embodiment 3: the detection of benzo (a) pyrene in 16 kinds of polycyclic aromatic hydrocarbon solutions

[0040] Pipette 2mL of the mixed solution containing 16 kinds of polycyclic aromatic hydrocarbons to draw the first-order derivative nonlinear variable-angle simultaneous fluorescence spectrum, and then continuously add 5, 15, 30, 45, 75, 110ng / mL anthracene standard solutions in the sample measurement solution , to draw the first-order layer-number nonlinear variable-angle synchronous fluorescence spectrum (such as Figure 6 Show). Read the fluorescence intensity of anthracene, draw the working curve with intensity versus concentration, and the slope is 14.01. Reading benzo(a)pyrene fluorescence intensity is 566.2, the quantitative formula that the slope, fluorescence intensity and relative correction factor 0.110 are substituted into the present invention is calculated and the concentration of benzo(a)pyrene is 4.46ng / mL, and reference value ( 4.5ng / mL) consiste...

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Abstract

A method for quantitating benzo (a) pyrene by adopting non-carcinogen anthracene as the standard relates to a detection method for benzo (a) pyrene. The method comprises the following steps: the step for acquiring a signal separation spectrum of benzo (a) pyrene and anthracene in a sample; the step for acquiring an anthracene standard addition method spectrum; the step for drawing a working curve; the step for reading the signal intensity of benzo (a) pyrene; and the step for calculating the concentration of benzo (a) pyrene in the sample. By adopting the method, benzo (a) pyrene can be quantitatively analyzed without needing benzo (a) pyrene standard solution, so that the probability that detection personnel contact benzo (a) pyrene (a kind of strong carcinogen) is effectively reduced; the health risk is accordingly reduced; the application value is significant; the spectrum can be simply and conveniently acquired only within one minute; and the price is cheap since valuable instruments such as an HPLC (High Performance Liquid Chromatograph), a gas chromatograph, a mass spectrograph and other expensive equipment are not needed.

Description

technical field [0001] The invention relates to a method for detecting benzo(a)pyrene, in particular to a method for quantitatively detecting benzo(a)pyrene by using non-carcinogen anthracene as a standard solution. Background technique [0002] Polycyclic aromatic hydrocarbons are mainly the products of incomplete combustion of coal, wood, fat and other substances. They are one of the earliest carcinogenic compounds discovered and studied. So far, the number of carcinogenic polycyclic aromatic hydrocarbons and their derivatives has reached 400 Various. Among them, benzo(a)pyrene is the most polluted, the most abundant, and the most carcinogenic. It is the most important environmental and food pollutant among the more than 200 kinds of PAHs that have been discovered, and has a certain correlation with other PAHs. , is used as an indicator and representative of environmental and food contamination by PAHs. Epidemiological studies have shown that benzo(a)pyrene can be absorb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 李耀群李秀英林丽容吴平平付东赛
Owner XIAMEN UNIV
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