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Seed tissue culture propagation method of dendrobium candidum

A technology of Dendrobium officinale and seed group, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of difficult collection of Dendrobium officinale seeds, low seed germination rate, uneconomical, etc. The effect of genetic shape stability and production cost reduction

Inactive Publication Date: 2013-09-25
重庆市秀山红星中药材开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because the seeds of Dendrobium candidum are difficult to collect, the germination rate of the seeds is low, and the seed propagation takes a long time to raise seedlings, which greatly increases the cultivation cost of Dendrobium candidum, which is not conducive to the artificial large-scale planting of Dendrobium candidum
Therefore, in the current artificial cultivation, propagation mainly relies on division propagation and cutting propagation, but this method has a low propagation coefficient, is not economical, and limits the yield potential of Dendrobium candidum, which is inconvenient for planting management and promotion, so the expansion of planting area is restrained
People have started artificial propagation, and many places have adopted tissue culture propagation methods to quickly obtain a large number of seedlings; at present, the propagation speed is very slow and cannot meet actual needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] First prepare the medium material:

[0020] Induction medium: N6 +NAA0.18mg / L+20% coconut milk+2% sucrose+activated carbon 200mg / L; pH=5.8.

[0021] Shape-promoting medium: N6 +NAA0.25mg / L+20% coconut milk+2% sucrose+activated carbon 200mg / L; pH=5.8.

[0022] Differentiation medium: N6+6~BA0.53mg / L+NAA0.20mg / L+10% banana juice+2% sucrose+activated carbon 200mg / L; pH=5.8.

[0023] Rooting medium: N6+IBA0.5mg / L+10% banana juice+2% sucrose+activated carbon 200mg / L; pH=5.6.

[0024] Select the uncracked ripe fruit of wild Dendrobium candidum, wash it under running water, put it in 1% detergent solution and stir it for 5 minutes, then rinse it with sterile water for 10 minutes; put the fruit in 70% alcohol on the ultra-clean workbench Disinfect for 20 seconds, then use 0.1% HgCl 2 Soak in aqueous solution for 7 minutes, then rinse with sterile water for 5 to 6 times; then cut the epidermis of the fruit in a sterile environment, cut the fruit into 0.1mm cubes, shake them i...

Embodiment 2

[0026] Select the uncracked ripe fruit of wild Dendrobium officinale, wash it under running water, put it into 1% detergent solution and stir it for 7 minutes, then rinse it with sterile water for 11 minutes; put the fruit in 70% alcohol on the ultra-clean workbench Disinfect for 20 seconds, then use 0.1% HgCl 2Soak in aqueous solution for 8 minutes, then rinse with sterile water for 5 to 6 times; then cut the epidermis of the fruit in a sterile environment, cut the fruit into 0.1mm squares, shake them in sterile water, shake evenly, and inoculate them with a medicine spoon. On the induction medium, inoculate 4 pieces per bottle, and conduct seed induction culture under the conditions of night temperature 22°C, day temperature 24°C, relative humidity 69%, and no light. After 15 to 20 days, the seeds begin to germinate and expand; continue to cultivate for 10 days. Days later, you can see the loose prototype of the protocorm; at this time, transfer the protocorm to the shape-pr...

Embodiment 3

[0028] Select the uncracked ripe fruit of wild Dendrobium officinale, wash it under running water, put it in 1% detergent solution and stir it for 8 minutes, then rinse it with sterile water for 12 minutes; put the fruit in 70% alcohol on the ultra-clean workbench Disinfect for 20 seconds, then use 0.1% HgCl 2 Soak in aqueous solution for 9 minutes, then rinse with sterile water for 5 to 6 times; then cut the epidermis of the fruit in a sterile environment, cut the fruit into 0.1mm squares, shake them in sterile water, shake evenly, and inoculate them with a spoon. On the induction medium, inoculate 5 pieces per bottle, and conduct seed induction culture under the conditions of night temperature 22°C, day temperature 24°C, relative humidity 70%, and no light. After 15 to 20 days, the seeds begin to germinate and expand; continue to cultivate for 10 days. Days later, you can see the loose prototype of the protocorm; at this time, transfer the protocorm to the shape-promoting me...

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Abstract

The invention relates to a seed tissue culture propagation method of dendrobium candidum and belongs to the technical field of planting of traditional Chinese medicinal materials. The seed tissue culture propagation method of dendrobium candidum comprises the following four steps of: introduction and sterilization, induction culture, differentiation culture as well as strong seedling and rooting culture. The seed tissue culture propagation method of dendrobium candidum has the beneficial effects that seedlings obtained by seed tissue culture are difficult to degenerate, genetic traits are stable, active substance content is close to that of a wild variety, and quality of seeds is guaranteed; quantity of the seeds of dendrobium candidum is high, and propagation quantity of the seeds can be greatly increased; the seed tissue culture propagation method of dendrobium candidum also greatly shortens a tissue culture propagation period of dendrobium candidum, the tissue culture period from seed shearing to rooting and seedling growing is only 4-5 months, production time is greatly shortened, and production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of planting Chinese medicinal materials, in particular to a seed tissue culture propagation method of dendrobium officinale. Background technique [0002] Dendrobium is a traditional precious Chinese herbal medicine in my country, which is recorded in all previous editions of Chinese Pharmacopoeia. The original plant of the traditional Chinese medicine Dendrobium is Dendrobium (Orchidaceae) ( Dendrobium Sw.) Herbaceous perennials. Dendrobium is one of the largest genera of Orchidaceae. There are more than 1,000 species in the world. There are 74 species and 2 varieties of Dendrobium plants in my country, of which more than 50 species are in commercial circulation. Among them, Dendrobium candidum is the most precious and the best of all Dendrobium. Dendrobium officinale is an endangered species, a national second-class key protected plant, and a rare and rare Chinese medicinal material. The ancient arist...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨再江刘湘凤顾君龚成珍祝利
Owner 重庆市秀山红星中药材开发有限公司
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